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Experiment Overview

Repository ID: FR-FCM-Z5Y3 Experiment name: Mod(mdg4)_Reporter_HETRP MIFlowCyt score: 15.00%
Primary researcher: Chikara Takeuchi PI/manager: Chikara Takeuchi Uploaded by: Chikara Takeuchi
Experiment dates: 2022-02-22 - Dataset uploaded: Feb 2022 Last updated: Feb 2022
Keywords: None Manuscripts:
Organizations: None
Purpose: Telomeres in Drosophila are composed of sequential non-LTR retrotransposons: HeTA, TART, and TAHRE. Although they are repressed by the piRNA pathway in the germline, how these retrotransposons are regulated in somatic cells is poorly understood. Here, we show that specific splice variants of Mod(mdg4) repress HeT-A by blocking subtelomeric enhancers in ovarian somatic cells. We found that the Mod(mdg4)-N variant represses HeT-A expression most efficiently among the variants. Mod(mdg4)-N mutant flies show elevated HeT-A expression and female sterility. Mod(mdg4)-N-binding subtelomeric sequences exhibit enhancer-blocking activity, and recruitment of RNA polymerase II (Pol II) on subtelomeres by Mod(mdg4)-N is essential for this enhancer-blocking. Moreover, Mod(mdg4)-N functions to form chromatin boundaries of higher-order chromatin conformation but this mechanism is independent of its Pol II recruitment activity at telomeres/subtelomeres. This study provides a link between enhancer-blocking and telomere regulation, and two different molecular mechanisms exhibited by an insulator protein to orchestrate precise gene expression.
Conclusion: None
Comments: None
Funding: Not disclosed
Quality control: None
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