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Experiment Overview

Repository ID: FR-FCM-Z5ZB Experiment name: Deciphering biological mechanisms involved in regulation by azithromycin of anti-tumor response after allogeneic hematopoietic stem cell transplantation MIFlowCyt score: 83.86%
Primary researcher: David Michonneau PI/manager: David Michonneau Uploaded by: David Michonneau
Experiment dates: 2020-06-02 - 2020-09-17 Dataset uploaded: Feb 2022 Last updated: Aug 2022
Keywords: [Cancer] [leukemia] [allogeneic hematopoietic stem cell transplantation] [relapse] [graft-versus-leukemia] [antibiotic] Manuscripts: [35984904]
Organizations: INSERM U976, Human immunology, pathophysiology and immunotherapy, Paris, (France)
Purpose: Measure azithromycin impact on immune subsets of patients included in the ALLOZITHRO study (NCT01959100).
Conclusion: These samples from 20 healthy donors and 204 patients included in the ALLOZITHRO study allowed the identification of specific changes associated with azithromycin intake. Paper in Blood: 10.1182/blood.2022016926
Comments: Source code of analyses is available on a Git repository: https://gitlab.com/nivall/azimut-blood
Funding: This work was supported by Leucémie Espoir, SOS oxygène, Pour Laurenne, and EGMOS association.
Quality control: FCS files obtained after acquisition were normalized following the bead-based procedure with ‘premessa’ R package using the graphical user interface function (github.com/ParkerICI/premessa). Removal of non-gaussian pulses and beads then identification of singlets and living cells were performed manually on FlowJo (version 10.8.1) on macOS (version 10.13.6). Retrieved events were then stored in new FCS files for further analyses and uploaded in this repository.


Experiment variables

Conditions
· AZM AZM_R1037.fcs · AZM_R1552.fcs · AZM_R1631.fcs · AZM_R1714.fcs · AZM_R1799.fcs · AZM_R1807.fcs · AZM_R1862.fcs · AZM_R1917.fcs · AZM_R1941.fcs · AZM_R1965.fcs · AZM_R2076.fcs · AZM_R2084.fcs · AZM_R2091.fcs · AZM_R2132.fcs · AZM_R2136.fcs · AZM_R2140.fcs · AZM_R2155.fcs · AZM_R2192.fcs · AZM_R2226.fcs · AZM_R2274.fcs · AZM_R2310.fcs · AZM_R2428.fcs · AZM_R2448.fcs · AZM_R2466.fcs · AZM_R2469.fcs · AZM_R2476.fcs · AZM_R2488.fcs · AZM_R2525.fcs · AZM_R2556.fcs · AZM_R2571.fcs · AZM_R2617.fcs · AZM_R2623.fcs · AZM_R2657.fcs · AZM_R2664.fcs · AZM_R2696.fcs · AZM_R2720.fcs · AZM_R2731.fcs · AZM_R2751.fcs · AZM_R2786.fcs · AZM_R2789.fcs · AZM_R2819.fcs · AZM_R2848.fcs · AZM_R2857.fcs · AZM_R2891.fcs · AZM_R2931.fcs · AZM_R2951.fcs · AZM_R2991.fcs · AZM_R2993.fcs · AZM_R2999.fcs · AZM_R3066.fcs · AZM_R3071.fcs · AZM_R3074.fcs · AZM_R3081.fcs · AZM_R3238.fcs · AZM_R3239.fcs · AZM_R3254.fcs · AZM_R3270.fcs · AZM_R3282.fcs · AZM_R3283.fcs · AZM_R3294.fcs · AZM_R3304.fcs · AZM_R3323.fcs · AZM_R3337.fcs · AZM_R3347.fcs · AZM_R3374.fcs · AZM_R3473.fcs · AZM_R3496.fcs · AZM_R3497.fcs · AZM_R3515.fcs · AZM_R3562.fcs · AZM_R3574.fcs · AZM_R3587.fcs · AZM_R3623.fcs · AZM_R3642.fcs · AZM_R3654.fcs · AZM_R3732.fcs · AZM_R3739.fcs · AZM_R3749.fcs · AZM_R3755.fcs · AZM_R3786.fcs · AZM_R3801.fcs · AZM_R3893.fcs · AZM_R3923.fcs · AZM_R3925.fcs · AZM_R3950.fcs · AZM_R3959.fcs · AZM_R4076.fcs · AZM_R4078.fcs · AZM_R4090.fcs · AZM_R4112.fcs · AZM_R4134.fcs · AZM_R4157.fcs · AZM_R4204.fcs · AZM_R4218.fcs · AZM_R4219.fcs · AZM_R4321.fcs · AZM_R4337.fcs · AZM_R4370.fcs
· PLA PLA_R1693.fcs · PLA_R1827.fcs · PLA_R1878.fcs · PLA_R1896.fcs · PLA_R1927.fcs · PLA_R2029.fcs · PLA_R2043.fcs · PLA_R2087.fcs · PLA_R2144.fcs · PLA_R2172.fcs · PLA_R2219.fcs · PLA_R2257.fcs · PLA_R2268.fcs · PLA_R2271.fcs · PLA_R2285.fcs · PLA_R2300.fcs · PLA_R2315.fcs · PLA_R2338.fcs · PLA_R2382.fcs · PLA_R2387.fcs · PLA_R2395.fcs · PLA_R2412.fcs · PLA_R2475.fcs · PLA_R2498.fcs · PLA_R2508.fcs · PLA_R2521.fcs · PLA_R2557.fcs · PLA_R2590.fcs · PLA_R2591.fcs · PLA_R2596.fcs · PLA_R2605.fcs · PLA_R2632.fcs · PLA_R2643.fcs · PLA_R2649.fcs · PLA_R2684.fcs · PLA_R2700.fcs · PLA_R2702.fcs · PLA_R2705.fcs · PLA_R2718.fcs · PLA_R2759.fcs · PLA_R2794.fcs · PLA_R2807.fcs · PLA_R2813.fcs · PLA_R2904.fcs · PLA_R2974.fcs · PLA_R2988.fcs · PLA_R2989.fcs · PLA_R3008.fcs · PLA_R3017.fcs · PLA_R3025.fcs · PLA_R3052.fcs · PLA_R3075.fcs · PLA_R3086.fcs · PLA_R3169.fcs · PLA_R3182.fcs · PLA_R3195.fcs · PLA_R3207.fcs · PLA_R3222.fcs · PLA_R3236.fcs · PLA_R3242.fcs · PLA_R3272.fcs · PLA_R3287.fcs · PLA_R3291.fcs · PLA_R3325.fcs · PLA_R3328.fcs · PLA_R3338.fcs · PLA_R3353.fcs · PLA_R3358.fcs · PLA_R3388.fcs · PLA_R3429.fcs · PLA_R3431.fcs · PLA_R3435.fcs · PLA_R3458.fcs · PLA_R3478.fcs · PLA_R3482.fcs · PLA_R3486.fcs · PLA_R3500.fcs · PLA_R3504.fcs · PLA_R3518.fcs · PLA_R3532.fcs · PLA_R3560.fcs · PLA_R3570.fcs · PLA_R3608.fcs · PLA_R3640.fcs · PLA_R3644.fcs · PLA_R3680.fcs · PLA_R3692.fcs · PLA_R3733.fcs · PLA_R3753.fcs · PLA_R3756.fcs · PLA_R3882.fcs · PLA_R3986.fcs · PLA_R3990.fcs · PLA_R4023.fcs · PLA_R4064.fcs · PLA_R4077.fcs · PLA_R4093.fcs · PLA_R4124.fcs · PLA_R4151.fcs · PLA_R4213.fcs · PLA_R4232.fcs · PLA_R4233.fcs · PLA_R4241.fcs · PLA_R4250.fcs · PLA_R4291.fcs · PLA_R4693.fcs
· HD HD_HD1.fcs · HD_HD10.fcs · HD_HD11.fcs · HD_HD12.fcs · HD_HD13.fcs · HD_HD14.fcs · HD_HD15.fcs · HD_HD16.fcs · HD_HD17.fcs · HD_HD18.fcs · HD_HD19.fcs · HD_HD2.fcs · HD_HD20.fcs · HD_HD3.fcs · HD_HD4.fcs · HD_HD5.fcs · HD_HD6.fcs · HD_HD7.fcs · HD_HD8.fcs · HD_HD9.fcs

Sample Type
· PBMC AZM_R1037.fcs · AZM_R1552.fcs · AZM_R1631.fcs · AZM_R1714.fcs · AZM_R1799.fcs · AZM_R1807.fcs · AZM_R1862.fcs · AZM_R1917.fcs · AZM_R1941.fcs · AZM_R1965.fcs · AZM_R2076.fcs · AZM_R2084.fcs · AZM_R2091.fcs · AZM_R2132.fcs · AZM_R2136.fcs · AZM_R2140.fcs · AZM_R2155.fcs · AZM_R2192.fcs · AZM_R2226.fcs · AZM_R2274.fcs · AZM_R2310.fcs · AZM_R2428.fcs · AZM_R2448.fcs · AZM_R2466.fcs · AZM_R2469.fcs · AZM_R2476.fcs · AZM_R2488.fcs · AZM_R2525.fcs · AZM_R2556.fcs · AZM_R2571.fcs · AZM_R2617.fcs · AZM_R2623.fcs · AZM_R2657.fcs · AZM_R2664.fcs · AZM_R2696.fcs · AZM_R2720.fcs · AZM_R2731.fcs · AZM_R2751.fcs · AZM_R2786.fcs · AZM_R2789.fcs · AZM_R2819.fcs · AZM_R2848.fcs · AZM_R2857.fcs · AZM_R2891.fcs · AZM_R2931.fcs · AZM_R2951.fcs · AZM_R2991.fcs · AZM_R2993.fcs · AZM_R2999.fcs · AZM_R3066.fcs · AZM_R3071.fcs · AZM_R3074.fcs · AZM_R3081.fcs · AZM_R3238.fcs · AZM_R3239.fcs · AZM_R3254.fcs · AZM_R3270.fcs · AZM_R3282.fcs · AZM_R3283.fcs · AZM_R3294.fcs · AZM_R3304.fcs · AZM_R3323.fcs · AZM_R3337.fcs · AZM_R3347.fcs · AZM_R3374.fcs · AZM_R3473.fcs · AZM_R3496.fcs · AZM_R3497.fcs · AZM_R3515.fcs · AZM_R3562.fcs · AZM_R3574.fcs · AZM_R3587.fcs · AZM_R3623.fcs · AZM_R3642.fcs · AZM_R3654.fcs · AZM_R3732.fcs · AZM_R3739.fcs · AZM_R3749.fcs · AZM_R3755.fcs · AZM_R3786.fcs · AZM_R3801.fcs · AZM_R3893.fcs · AZM_R3923.fcs · AZM_R3925.fcs · AZM_R3950.fcs · AZM_R3959.fcs · AZM_R4076.fcs · AZM_R4078.fcs · AZM_R4090.fcs · AZM_R4112.fcs · AZM_R4134.fcs · AZM_R4157.fcs · AZM_R4204.fcs · AZM_R4218.fcs · AZM_R4219.fcs · AZM_R4321.fcs · AZM_R4337.fcs · AZM_R4370.fcs · HD_HD1.fcs · HD_HD10.fcs · HD_HD11.fcs · HD_HD12.fcs · HD_HD13.fcs · HD_HD14.fcs · HD_HD15.fcs · HD_HD16.fcs · HD_HD17.fcs · HD_HD18.fcs · HD_HD19.fcs · HD_HD2.fcs · HD_HD20.fcs · HD_HD3.fcs · HD_HD4.fcs · HD_HD5.fcs · HD_HD6.fcs · HD_HD7.fcs · HD_HD8.fcs · HD_HD9.fcs · PLA_R1693.fcs · PLA_R1827.fcs · PLA_R1878.fcs · PLA_R1896.fcs · PLA_R1927.fcs · PLA_R2029.fcs · PLA_R2043.fcs · PLA_R2087.fcs · PLA_R2144.fcs · PLA_R2172.fcs · PLA_R2219.fcs · PLA_R2257.fcs · PLA_R2268.fcs · PLA_R2271.fcs · PLA_R2285.fcs · PLA_R2300.fcs · PLA_R2315.fcs · PLA_R2338.fcs · PLA_R2382.fcs · PLA_R2387.fcs · PLA_R2395.fcs · PLA_R2412.fcs · PLA_R2475.fcs · PLA_R2498.fcs · PLA_R2508.fcs · PLA_R2521.fcs · PLA_R2557.fcs · PLA_R2590.fcs · PLA_R2591.fcs · PLA_R2596.fcs · PLA_R2605.fcs · PLA_R2632.fcs · PLA_R2643.fcs · PLA_R2649.fcs · PLA_R2684.fcs · PLA_R2700.fcs · PLA_R2702.fcs · PLA_R2705.fcs · PLA_R2718.fcs · PLA_R2759.fcs · PLA_R2794.fcs · PLA_R2807.fcs · PLA_R2813.fcs · PLA_R2904.fcs · PLA_R2974.fcs · PLA_R2988.fcs · PLA_R2989.fcs · PLA_R3008.fcs · PLA_R3017.fcs · PLA_R3025.fcs · PLA_R3052.fcs · PLA_R3075.fcs · PLA_R3086.fcs · PLA_R3169.fcs · PLA_R3182.fcs · PLA_R3195.fcs · PLA_R3207.fcs · PLA_R3222.fcs · PLA_R3236.fcs · PLA_R3242.fcs · PLA_R3272.fcs · PLA_R3287.fcs · PLA_R3291.fcs · PLA_R3325.fcs · PLA_R3328.fcs · PLA_R3338.fcs · PLA_R3353.fcs · PLA_R3358.fcs · PLA_R3388.fcs · PLA_R3429.fcs · PLA_R3431.fcs · PLA_R3435.fcs · PLA_R3458.fcs · PLA_R3478.fcs · PLA_R3482.fcs · PLA_R3486.fcs · PLA_R3500.fcs · PLA_R3504.fcs · PLA_R3518.fcs · PLA_R3532.fcs · PLA_R3560.fcs · PLA_R3570.fcs · PLA_R3608.fcs · PLA_R3640.fcs · PLA_R3644.fcs · PLA_R3680.fcs · PLA_R3692.fcs · PLA_R3733.fcs · PLA_R3753.fcs · PLA_R3756.fcs · PLA_R3882.fcs · PLA_R3986.fcs · PLA_R3990.fcs · PLA_R4023.fcs · PLA_R4064.fcs · PLA_R4077.fcs · PLA_R4093.fcs · PLA_R4124.fcs · PLA_R4151.fcs · PLA_R4213.fcs · PLA_R4232.fcs · PLA_R4233.fcs · PLA_R4241.fcs · PLA_R4250.fcs · PLA_R4291.fcs · PLA_R4693.fcs

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