Experiment Overview
| Repository ID: | FR-FCM-Z5ZW | Experiment name: | Analysis of TC-mAb mice (Exp2: Transitonal B, Mature B)) | MIFlowCyt score: | 30.66% |
| Primary researcher: | Takashi Moriwaki | PI/manager: | Yasuhiro Kazuki | Uploaded by: | Takashi Moriwaki |
| Experiment dates: | 2021-04-21 - 2021-04-28 | Dataset uploaded: | Feb 2022 | Last updated: | Mar 2022 |
| Keywords: | None | Manuscripts: | |||
| Organizations: |
Tottori University, Yonago, (Japan)
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| Purpose: | The experiment had been performed to analyze B cell development of human antibody-procducing mice (TC-mAb mice). The flow cytometry experiment was designed to detect the subsets of Follicular B (CD19+CD93-CD21loCD23hi), marginal zone B (CD19+CD93-CD21hiCD23lo) cells, transitional (T1 (CD19+CD93+IgMhiCD23-), T2 (CD19+CD93+IgMhiCD23+), and T3 (CD19+CD93+IgMloCD23+) in Spleen and Lymph node. In addditon, the expression pattern of IgM and IgD in Igk+ follicular B cells was also analyzed. | ||||
| Conclusion: | None | ||||
| Comments: | This experiment corresponds to Figure 7b, c and Supplementary Figure 17-20 in the paper "Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci". Publication link: https://doi.org/10.21203/rs.3.rs-103241/v1 | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
