Experiment Overview
| Repository ID: | FR-FCM-Z632 | Experiment name: | Insights into the enumeration of mixtures of probiotic bacteria by flow cytometry | MIFlowCyt score: | 24.00% |
| Primary researcher: | Niall Coates | PI/manager: | Niall Coates | Uploaded by: | Niall Coates |
| Experiment dates: | 2023-02-07 - 2023-02-07 | Dataset uploaded: | Feb 2023 | Last updated: | Feb 2023 |
| Keywords: | [cell viability] [Bacteria] [Lactobacillus] [probiotic] [multispecies] [bifidobacteria] | Manuscripts: | |||
| Organizations: | None | ||||
| Purpose: | To investigate the suitability of a general gating strategy for enumeration of multi-species probiotic blends with live/dead staining. Gating strategy was designed based on cytograms of individual freeze-dried probiotic preparations (CUL21/60, CUL20/34, CUL08, CUL66N and CUL61) before being applied to different combinations of these organisms. FC results were compared to plate counts. | ||||
| Conclusion: | Live/dead flow cytometry can be used to enumerate single and mixed populations of probiotic bacteria more accurately/reliably than the gold standard plate count technique. However the accuracy of multi-species enumeration is impacted by the composition of the blend with the potential of FC to over-enumerate in some cases. However, the over-enumeration of FC is not as severe as the under-enumeration by plate counts. We conclude that any effort to gate multi-species products must first assess the gating strategy on the individual bacterial components to determine potential inaccuracies in the method. | ||||
| Comments: | Only 1 replicate of each batch of individual freeze-dried preparation and multi-species blend is uploaded. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
