Purpose:
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We designed this panel to deeply immunophenotype major leukocyte populations in primary and secondary murine lymphoid tissues as a function of time and treatment. This panel uses a robust set of extracellular markers capable of differentiating leukocyte subsets without the use of intracellular staining, thus allowing for the use of cells in downstream functional experiments or multiomic analyses. Our panel classifies T cells, B cells, natural killer cells, innate lymphoid cells, monocytes, macrophages, dendritic cells, basophils, neutrophils, eosinophils, progenitors, and their functional subsets by using a series of co-stimulatory, checkpoint, activation, migration, and maturation markers. Importantly, this panel can be used in pre-clinical settings to understand how diseases and corresponding treatments modulate leukocyte abundance and/or function in a systems wide setting. |