Experiment Overview
| Repository ID: | FR-FCM-Z64A | Experiment name: | Multi-color flow cytometry bovine AT-MSCs | MIFlowCyt score: | 50.00% |
| Primary researcher: | Heyman Emma | PI/manager: | Heyman Emma | Uploaded by: | Heyman Emma |
| Experiment dates: | 2018-12-30 - | Dataset uploaded: | Mar 2023 | Last updated: | Mar 2023 |
| Keywords: | [Multi-color flow cytometry] [heterogeneity ] [Cattle] [MSC characterization] [cross-reactivity] | Manuscripts: | |||
| Organizations: |
Faculty of Veterinary Medicine (Ghent University), Translational Physiology, Infectiology and Public Health, Merelbeke, (Belgium)
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| Purpose: | In this study, we aimed to immunophenotype bovine adipose tissue (AT)-derived MSCs using multi-color flow cytometry. 13 commercial Abs were screened for recognizing bovine epitopes using the appropriate positive controls. | ||||
| Conclusion: | Using flow cytometry and immunofluorescence microscopy, cross-reactivity was confirmed for CD34, CD73, CD79α and CD90. Unfortunately, none of the evaluated CD105 and CD106 Abs cross-reacted with bovine cells. Subsequently, AT-derived bovine MSCs were characterized using multi-color flow cytometry based on their expression of nine markers. Bovine MSCs clearly expressed CD29 and CD44, and lacked expression of CD14, CD45, CD73, CD79α and MHCII, while a variable expression was observed for CD34 and CD90. In addition, the mRNA transcription level of different markers was analyzed using RT-qPCR. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | For daily validation of the flow cytometer, CytoFlex Daily QC Fluorospheres (Beckman Coulter, B53230) are used. For weekly validation of the flow cytometer, an 8-peak validation is performed using rainbow calibration beads (Spherotech, RCP-30-5A). | ||||
