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Experiment Overview
Repository ID: | FR-FCM-Z64Q | Experiment name: | Investigation of [NADH:NAD+] of E. coli cells prior/post ciprofloxacin exposure with Peredox biosensor | MIFlowCyt score: | 42.50% |
Primary researcher: | Joanna Urbaniec | PI/manager: | Joanna Urbaniec | Uploaded by: | Joanna Urbaniec |
Experiment dates: | 2022-04-01 - 2023-07-01 | Dataset uploaded: | Mar 2023 | Last updated: | Jun 2023 |
Keywords: | None | Manuscripts: | |||
Organizations: | None | ||||
Purpose: | To investigate redox homeostasis of ciprofloxacin-sensitive and ciprofloxacin-tolerant E. coli cells on as single-cell level, with the use of Peredox NADH:NAD+ biosensor. | ||||
Conclusion: | Ciprofloxacin-tolerant cells have a significatly lower NADH:NAD+ ratio than the bulk population prior to antibiotic exposure; this difference is also signficant between ciprofloxacin-tolerant cells of a highly-persistent strain E. coli HipQ, and ciprofloxacin-olerant cells of its parental strain | ||||
Comments: | None | ||||
Funding: | Not disclosed | ||||
Quality control: | Data from 3 independent experiments; controlled for autofluorescence for each timepoint to strains not transformed with the Peredox biosensor; compensation not applied as fluorophores did not overlap |