Experiment Overview
| Repository ID: | FR-FCM-Z64Q | Experiment name: | Investigation of [NADH:NAD+] of E. coli cells prior/post ciprofloxacin exposure with Peredox biosensor | MIFlowCyt score: | 42.50% |
| Primary researcher: | Joanna Urbaniec | PI/manager: | Joanna Urbaniec | Uploaded by: | Joanna Urbaniec |
| Experiment dates: | 2022-04-01 - 2023-07-01 | Dataset uploaded: | Mar 2023 | Last updated: | Jun 2023 |
| Keywords: | None | Manuscripts: | |||
| Organizations: | None | ||||
| Purpose: | To investigate redox homeostasis of ciprofloxacin-sensitive and ciprofloxacin-tolerant E. coli cells on as single-cell level, with the use of Peredox NADH:NAD+ biosensor. | ||||
| Conclusion: | Ciprofloxacin-tolerant cells have a significatly lower NADH:NAD+ ratio than the bulk population prior to antibiotic exposure; this difference is also signficant between ciprofloxacin-tolerant cells of a highly-persistent strain E. coli HipQ, and ciprofloxacin-olerant cells of its parental strain | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Data from 3 independent experiments; controlled for autofluorescence for each timepoint to strains not transformed with the Peredox biosensor; compensation not applied as fluorophores did not overlap | ||||
