Experiment Overview
| Repository ID: | FR-FCM-Z66K | Experiment name: | Molecular analysis and comparison of CD34+ and CD133+ very small embryonic-like stem cells (VSELs) purified from umbilical cord blood (UCB) ? Brief Report. | MIFlowCyt score: | 81.31% |
| Primary researcher: | Kamila Bujko | PI/manager: | Kamila Bujko | Uploaded by: | Kamila Bujko |
| Experiment dates: | 2022-08-01 - 2022-10-31 | Dataset uploaded: | Apr 2023 | Last updated: | Apr 2023 |
| Keywords: | [HSC] [Very Small Embryonic-Like Stem Cells (VSELs)] [FACS Verse] | Manuscripts: | |||
| Organizations: |
Warsaw Medical University, Laboratory of Regenerative Medicine, Warsaw, (Poland)
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| Purpose: | The purpose of flow cytometry analysis was to enumerate CD133+ and CD34+ Very Small Embryonic-Like Stem Cells (VSELs) in human umbilical cord blood (hUCB) and to analyze the coexpression of both markers. | ||||
| Conclusion: | 13 samples were analyzed and average number of in-CD45-CD133+ and Lin-CD45-CD34+ VSELs in hUCB was calculated as well as coexpression of CD133 and CD34 markers. | ||||
| Comments: | The reposited FCS files contain a representative dot plots used to enumerate Lin-CD45-CD133+ and Lin-CD45-CD34+ VSELs in hUCB. Data were aquired on The BD FACSVerse? instrument. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | The flow cytometer automatic compensation was performed. | ||||
