Experiment Overview
| Repository ID: | FR-FCM-Z6CJ | Experiment name: | Human Lung Digestion Epithelial | MIFlowCyt score: | 39.50% |
| Primary researcher: | Mark Snyder | PI/manager: | Mark Snyder | Uploaded by: | Mark Snyder |
| Experiment dates: | 2021-05-12 - | Dataset uploaded: | May 2023 | Last updated: | Jun 2023 |
| Keywords: | None | Manuscripts: | |||
| Organizations: |
University of Pittsburgh School of Medicine, Medicine, Pittsburgh, Pennsylvania (United States)
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| Purpose: | To investigate the impact of different enzymatic digestion protocols on non-immune cell yield and phenotype from human lung tissue. | ||||
| Conclusion: | None | ||||
| Comments: | Protocol 1: Mechanical digestion alone (gentleMACS) Protocol 2: Liberase + Dnase 1 (Dispase lo) Protocol 3: Dispase + Col D + Dnase 1 (Dispase hi) Protocol 4: Col P + Dnase 1 (Col_P) Protocol 5: Col D + Dnase 1 + Trypsin inhibitor (Col_D) Protocol 6: Protocol 5 + density centrifugation (Col_D+) | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
