Experiment Overview
| Repository ID: | FR-FCM-Z6KA | Experiment name: | Yeast DNA stain WT vs naa10-delta | MIFlowCyt score: | 50.75% |
| Primary researcher: | Henriette Aksnes | PI/manager: | Henriette Aksnes | Uploaded by: | Henriette Aksnes |
| Experiment dates: | 2023-03-15 - 2023-04-18 | Dataset uploaded: | Jul 2023 | Last updated: | Jul 2023 |
| Keywords: | [budding yeast] [naa10] [Flow Cytometry; Nuclear DNA content ] [N-terminal acetylation] | Manuscripts: | [37500638] [PMC10374663] | ||
| Organizations: |
University of Bergen, Department of Biomedicine, Bergen, (Norway)
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| Purpose: | 1. Investigate the cell cycle distribution in actively growing naa10? vs WT yeast based on SYTOX Green DNA stain. 2. Investigate the cell size of actively growing naa10? vs WT yeast based on forward scatter measurements. | ||||
| Conclusion: | 1. Actively growing WT and naa10Δ yeast cells showed no significant difference between their cell cycle distribution profiles. 2. naa10Δ cells were 20% larger than WT cells. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Sample Type | |
|---|---|
| · WT experiment 1 | A03 36sc_20.3.fcs |
| · WT experiment 2 | A01 36 A.fcs · A02 36 B.fcs · A03 36 C.fcs |
| · WT experiment 3 | A01 36A.fcs · A02 36B.fcs · A03 36C.fcs |
| · naa10-del experiment 1 | A04 42sc_20.3.fcs |
| · naa10-del experiment 2 | A04 42 A.fcs · A05 42 B.fcs · A06 42 C.fcs |
| · naa10-del experiment 3 | A04 42A.fcs · A05 42B.fcs · A06 42C.fcs |
