Experiment Overview
| Repository ID: | FR-FCM-Z6KP | Experiment name: | Expression of cDC1-associated surface markers on XCR1- and XCR1+ cDC1 | MIFlowCyt score: | 80.11% |
| Primary researcher: | Lukas Heger | PI/manager: | Diana Dudziak | Uploaded by: | Lukas Heger |
| Experiment dates: | 2023-03-16 - 2023-03-16 | Dataset uploaded: | Jul 2023 | Last updated: | Sep 2023 |
| Keywords: | [human PBMCs] [XCR1] [cDC1] | Manuscripts: | [37566635] [PMC10438835] | ||
| Organizations: |
Uniklinikum Erlangen, Department of Dermatology, Laboratory of DC Biology, Erlangen, (Germany)
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| Purpose: | In order to test whether XCR1- and XCR1+ cDC1 differ in the expression of cDC1-associated surface markers, expression of BTLA, CD5, CD13, CD26, CD205, CD226, CLEC7A and CLEC9A were analyzed by flow cytometry on PBMCs of healthy human blood donors. Further, XCR1 expression was analyzed on immune cells in different tissues. | ||||
| Conclusion: | XCR1- and XCR1+ cDC1 showed comparable expression of cDC1-associated surface markers. | ||||
| Comments: | Published in: Proceedings of the National Academy of Sciences (PNAS), 2023 Title: XCR1 expression distinguishes human conventional dendritic cell type 1 with full effector function from their immediate precursors. Authors: Lukas Heger, Lukas Hatscher, Chunguang Liang, Christian H. K. Lehmann, Lukas Amon, Jennifer J. Lühr, Tomasz Kaszubowski, Rayk Nzirorera, Niels Schaft, Jan Dörrie, Pascal Irrgang, Matthias Tenbusch, Meik Kunz, Eileen Socher, Stella E. Autenrieth, Ariawan Purbojo, Horia Sirbu, Arndt Hartmann, Christoph Alexiou, Robert Cesnjevar, and Diana Dudziak doi: 10.1073/pnas.2300343120 | ||||
| Funding: | This work was partly supported by grants from the German Research Foundation [Deutsche Forschungsgemeinschaft (DFG)] to Diana Dudziak (CRC1181-TPA7, DU548/5-1) and a proposal funded by the Agence Nationale de la Recherche (ANR) and the DFG (DU548/6-1). Diana Dudziak received support from Interdisziplinäres Zentrum für Klinische Forschung (IZKF) (IZKF-A80). Lukas Heger was supported by Erlanger Leistungsbezogene Anschubfinanzierung und Nachwuchsförderung (ELAN) (DE-17-09-15-1-Heger). Diana Dudziak was funded by the Bavarian State of Ministry of Science and Art, Bayresq.Net. | ||||
| Quality control: | Isotype controls for CD5, CD13, CD26, CD205, CD226, and CLEC7A | ||||
