Experiment Overview
| Repository ID: | FR-FCM-Z6KV | Experiment name: | Cytokine secretion by sorted XCR1- and XCR1+ cDC1 | MIFlowCyt score: | 79.50% |
| Primary researcher: | Lukas Heger | PI/manager: | Diana Dudziak | Uploaded by: | Lukas Heger |
| Experiment dates: | 2017-05-15 - 2017-06-30 | Dataset uploaded: | Jul 2023 | Last updated: | Sep 2023 |
| Keywords: | [IFNg] [TNF] [GM-CSF] [IL-10] [IL-6] [IL-8] [cDC1] [IFNa] [IL-1b] [IL-12p70] [IL-12/IL-23p40] [IP-10] [MIP-1a] [MIP-1b] [RANTES] [IFNl1] [IFNl2/3] [IFNb] | Manuscripts: | [37566635] [PMC10438835] | ||
| Organizations: |
Uniklinikum Erlangen, Department of Dermatology, Laboratory of DC Biology, Erlangen, (Germany)
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| Purpose: | In order to analyze whether XCR1- and XCR1+ cDC1 differ in the secretion of cytokines, XCR1- and XCR1+ cDC1 were isolated from blood of healthy donors by cell-sorting and subsequently stimulated with TLR ligands for 12h in vitro. Concentration of secreted cytokines were measured in the supernatant of the cells by CBA assay using FlexSets (BD Biosciences) and the LEGENDplex Human Anti-Virus Response Panel (BioLegend). | ||||
| Conclusion: | XCR1+ cDC1 excel in the secretion of secreted cytokines. Stimulation with pIC induces different repertoire of cytokines than stimulation with R848. | ||||
| Comments: | Published in: Proceedings of the National Academy of Sciences (PNAS), 2023 Title: XCR1 expression distinguishes human conventional dendritic cell type 1 with full effector function from their immediate precursors. Authors: Lukas Heger, Lukas Hatscher, Chunguang Liang, Christian H. K. Lehmann, Lukas Amon, Jennifer J. Lühr, Tomasz Kaszubowski, Rayk Nzirorera, Niels Schaft, Jan Dörrie, Pascal Irrgang, Matthias Tenbusch, Meik Kunz, Eileen Socher, Stella E. Autenrieth, Ariawan Purbojo, Horia Sirbu, Arndt Hartmann, Christoph Alexiou, Robert Cesnjevar, and Diana Dudziak doi: 10.1073/pnas.2300343120 | ||||
| Funding: | This work was partly supported by grants from the German Research Foundation [Deutsche Forschungsgemeinschaft (DFG)] to Diana Dudziak (CRC1181-TPA7, DU548/5-1) and a proposal funded by the Agence Nationale de la Recherche (ANR) and the DFG (DU548/6-1). Diana Dudziak received support from Interdisziplinäres Zentrum für Klinische Forschung (IZKF) (IZKF-A80). Lukas Heger was supported by Erlanger Leistungsbezogene Anschubfinanzierung und Nachwuchsförderung (ELAN) (DE-17-09-15-1-Heger). Diana Dudziak was funded by the Bavarian State of Ministry of Science and Art, Bayresq.Net. | ||||
| Quality control: | Standard curves using lyophilized cytokines. | ||||
