Experiment Overview
| Repository ID: | FR-FCM-Z6MY | Experiment name: | Inhibition of viral replication by XCR1- and XCR1+ cDC1 | MIFlowCyt score: | 79.50% |
| Primary researcher: | Lukas Heger | PI/manager: | Diana Dudziak | Uploaded by: | Lukas Heger |
| Experiment dates: | 2018-05-28 - 2018-08-09 | Dataset uploaded: | Jul 2023 | Last updated: | Sep 2023 |
| Keywords: | [XCR1] [cDC1] [Interferon] | Manuscripts: | [37566635] [PMC10438835] | ||
| Organizations: |
Uniklinikum Erlangen, Department of Dermatology, Laboratory of DC Biology, Erlangen, (Germany)
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| Purpose: | In order to analyze whether type I and III IFN secreted by XCR1- and XCR1+ cDC1 inhibit viral replication, sorted XCR1- and XCR1+ cDC1 were stimulated with pIC for 12h. Supernatants were harvested and used to induce an antiviral state in U2OS cells. Concentration of IFN was determined in the supernatants using the LEGENDplex Human Type 1/2/3 Interferon Panel (5-plex) with V-bottom Plate. | ||||
| Conclusion: | XCR1+ cDC1 excel in the secretion of type I and III IFN, which correlates with the superior induction of an antiviral state in epithelial cells. | ||||
| Comments: | Published in: Proceedings of the National Academy of Sciences (PNAS), 2023 Title: XCR1 expression distinguishes human conventional dendritic cell type 1 with full effector function from their immediate precursors. Authors: Lukas Heger, Lukas Hatscher, Chunguang Liang, Christian H. K. Lehmann, Lukas Amon, Jennifer J. Lühr, Tomasz Kaszubowski, Rayk Nzirorera, Niels Schaft, Jan Dörrie, Pascal Irrgang, Matthias Tenbusch, Meik Kunz, Eileen Socher, Stella E. Autenrieth, Ariawan Purbojo, Horia Sirbu, Arndt Hartmann, Christoph Alexiou, Robert Cesnjevar, and Diana Dudziak doi: 10.1073/pnas.2300343120 | ||||
| Funding: | This work was partly supported by grants from the German Research Foundation [Deutsche Forschungsgemeinschaft (DFG)] to Diana Dudziak (CRC1181-TPA7, DU548/5-1) and a proposal funded by the Agence Nationale de la Recherche (ANR) and the DFG (DU548/6-1). Diana Dudziak received support from Interdisziplinäres Zentrum für Klinische Forschung (IZKF) (IZKF-A80). Lukas Heger was supported by Erlanger Leistungsbezogene Anschubfinanzierung und Nachwuchsförderung (ELAN) (DE-17-09-15-1-Heger). Diana Dudziak was funded by the Bavarian State of Ministry of Science and Art, Bayresq.Net. | ||||
| Quality control: | Standard curve using lyophylized proteins. | ||||
