Experiment Overview
| Repository ID: | FR-FCM-Z6N6 | Experiment name: | Fluorochrome-to-protein (F/P) ratio affects Tissue Factor (TF) detection on extracellular vesicles (EVs) | MIFlowCyt score: | 51.50% |
| Primary researcher: | Marwa Mostageer | PI/manager: | Marwa Mostageer | Uploaded by: | Marwa Mostageer |
| Experiment dates: | 2022-10-13 - 2023-05-24 | Dataset uploaded: | Aug 2023 | Last updated: | Aug 2023 |
| Keywords: | [flow cytometry] [Extracellular vesicles] [fluorochrome-to-protein ratio] [tissue factor] | Manuscripts: | [38494537] |
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| Organizations: |
University for Continuing Education Krems, Department for Biomedical Research, Krems, (Austria)
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| Purpose: | We investigated the effect of different fluorochrome-to-protein (F/P) ratios of anti-TF-fluorochrome conjugates on the flow cytometric detection of TF-positive EVs from activated monocytes, mesenchymal stem cells (MSCs), and in COVID-19 plasma. | ||||
| Conclusion: | We show for the first time that the F/P ratio of fluorochrome-antibody conjugates is critical for the detection of TF on EVs, while it does not affect the detection of TF on cells. | ||||
| Comments: | None | ||||
| Funding: | This research was funded by the Technology Fund of Lower Austria and the Lower Austrian Society for Research Funding (GFF) under the grant number K3-F-744/005-2019. | ||||
| Quality control: | Quality control beads (CytoFLEX Daily QC Fluorospheres) were run prior to each measurement. | ||||
