Experiment Overview
| Repository ID: | FR-FCM-Z6U4 | Experiment name: | Neutrophil Conserved Inflammatory Response (cross organ) | MIFlowCyt score: | 31.50% |
| Primary researcher: | Tarik Exner | PI/manager: | Tarik Exner | Uploaded by: | Tarik Exner |
| Experiment dates: | 2022-04-10 - 2022-04-24 | Dataset uploaded: | Oct 2023 | Last updated: | Nov 2023 |
| Keywords: | [Neutrophil] [Human Mouse] [inflamm] | Manuscripts: | |||
| Organizations: |
University of Heidelberg, Institute for Immunology, Heidelberg, (Germany)
|
||||
| Purpose: | These studies were conducted to confirm expression changes of inflamed neutrophils that were quantified by bulk-RNA-seq data on a surface protein level. Neutrophils from three different organs (bone marrow, blood and spleen) were compared. | ||||
| Conclusion: | Surface expression of PD-L1, CD40, CD14, IL4R and CD69 could be shown to be increased upon in vitro stimulation with GM-CSF + interferon-gamma and GM-CSF + LPS. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Single stains for the relevant markers, unstained samples for population identification. | ||||
Experiment variables
| Conditions | |
|---|---|
| · GI | experiment_II_0_M4_BM_GI.fcs · experiment_II_0_M4_PB_GI.fcs · experiment_II_0_M4_SPL_GI.fcs · experiment_II_0_M5_BM_GI.fcs · experiment_II_0_M5_PB_GI.fcs · experiment_II_0_M5_SPL_GI.fcs · experiment_II_0_M6_BM_GI.fcs · experiment_II_0_M6_PB_GI.fcs · experiment_II_0_M6_SPL_GI.fcs · experiment_I_0_M1_KM_GI.fcs · experiment_I_0_M1_PB_GI.fcs · experiment_I_0_M1_SPL_GI.fcs · experiment_I_0_M2_BM_GI.fcs · experiment_I_0_M2_PB_GI.fcs · experiment_I_0_M2_SPL_GI.fcs · experiment_I_0_M3_BM_GI.fcs · experiment_I_0_M3_PB_GI.fcs · experiment_I_0_M3_SPL_GI.fcs |
| · GL | experiment_II_0_M4_BM_GL.fcs · experiment_II_0_M4_PB_GL.fcs · experiment_II_0_M4_SPL_GL.fcs · experiment_II_0_M5_BM_GL.fcs · experiment_II_0_M5_PB_GL.fcs · experiment_II_0_M5_SPL_GL.fcs · experiment_II_0_M6_BM_GL.fcs · experiment_II_0_M6_PB_GL.fcs · experiment_II_0_M6_SPL_GL.fcs · experiment_I_0_M1_KM_GL.fcs · experiment_I_0_M1_PB_GL.fcs · experiment_I_0_M1_SPL_GL.fcs · experiment_I_0_M2_BM_GL.fcs · experiment_I_0_M2_PB_GL.fcs · experiment_I_0_M2_SPL_GL.fcs · experiment_I_0_M3_BM_GL.fcs · experiment_I_0_M3_PB_GL.fcs · experiment_I_0_M3_SPL_GL.fcs |
| · unstim | experiment_II_0_M4_BM_unstim.fcs · experiment_II_0_M4_BM_unstim_unstained.fcs · experiment_II_0_M4_PB_unstim.fcs · experiment_II_0_M4_PB_unstim_unstained.fcs · experiment_II_0_M4_SPL_unstim.fcs · experiment_II_0_M4_SPL_unstim_unstained.fcs · experiment_II_0_M5_BM_unstim.fcs · experiment_II_0_M5_BM_unstim_unstained.fcs · experiment_II_0_M5_PB_unstim.fcs · experiment_II_0_M5_PB_unstim_unstained.fcs · experiment_II_0_M5_SPL_unstim.fcs · experiment_II_0_M5_SPL_unstim_unstained.fcs · experiment_II_0_M6_BM_unstim.fcs · experiment_II_0_M6_BM_unstim_unstained.fcs · experiment_II_0_M6_PB_unstim.fcs · experiment_II_0_M6_PB_unstim_unstained.fcs · experiment_II_0_M6_SPL_unstim.fcs · experiment_II_0_M6_SPL_unstim_unstained.fcs · experiment_I_0_M1_KM_unstim.fcs · experiment_I_0_M1_KM_unstim_unstained.fcs · experiment_I_0_M1_PB_unstim.fcs · experiment_I_0_M1_PB_unstim_unstained.fcs · experiment_I_0_M1_SPL_unstim.fcs · experiment_I_0_M1_SPL_unstim_unstained.fcs · experiment_I_0_M2_BM_unstim.fcs · experiment_I_0_M2_BM_unstim_unstained.fcs · experiment_I_0_M2_PB_unstim.fcs · experiment_I_0_M2_PB_unstim_unstained.fcs · experiment_I_0_M2_SPL_unstim.fcs · experiment_I_0_M2_SPL_unstim_unstained.fcs · experiment_I_0_M3_BM_unstim.fcs · experiment_I_0_M3_BM_unstim_unstained.fcs · experiment_I_0_M3_PB_unstim.fcs · experiment_I_0_M3_PB_unstim_unstained.fcs · experiment_I_0_M3_SPL_unstim.fcs · experiment_I_0_M3_SPL_unstim_unstained.fcs |
