Experiment Overview
| Repository ID: | FR-FCM-Z6UG | Experiment name: | Determining the role of CD4+ and CD8+ T-cells in the LST response | MIFlowCyt score: | 100.00% |
| Primary researcher: | Jalal Alshaweesh | PI/manager: | Shinjiro Hamano | Uploaded by: | Jalal Alshaweesh |
| Experiment dates: | 2019-04-01 - 2023-10-01 | Dataset uploaded: | Oct 2023 | Last updated: | Oct 2023 |
| Keywords: | [CD3+CD4+] [CD3+CD8+] [lymphodepletion] [Leishmanin] [LST] | Manuscripts: | |||
| Organizations: |
Nagasaki University, Department of Parasitology, Nagasaki , Nagasaki (Japan)
|
||||
| Purpose: | Validate the Leishmania donovani LST leishmanin antigen in immune animals. | ||||
| Conclusion: | The cell depletion was confirmed in sampled mice using flow cytometry and Rag2-/- mice were used as a control for the depletion of CD4+ and CD8+ T-cells. | ||||
| Comments: | None | ||||
| Funding: | The Global Health Innovative Technology Fund (GHIT) | ||||
| Quality control: | Experiment included: Negative control, Positive control and FMO controls | ||||
Experiment variables
| Conditions | |
|---|---|
| · FMO control | Spleenocytes_Tcells_FMO- no CD4 staining_002.fcs |
| · Unstained | Spleenocytes_Tcells_Unstained_control_001.fcs |
| · Undepleted | Spleenocytes_Tcells_Vaccinated_Saline_004.fcs |
| · Rag2 KO control | Spleenocytes_Tcells_Rag2KO_005.fcs |
| · Samples | Spleenocytes_Tcells_Vaccinated_GK15_006.fcs · Spleenocytes_Tcells_Vaccinated_GK15__2_007.fcs · Spleenocytes_Tcells_Vaccinated_GK15__3_009.fcs · Spleenocytes_Tcells_Vaccinated_aCD8_003.fcs |
| Sample Type | |
|---|---|
| · Spleenocytes | Spleenocytes_Tcells_FMO- no CD4 staining_002.fcs · Spleenocytes_Tcells_Rag2KO_005.fcs · Spleenocytes_Tcells_Unstained_control_001.fcs · Spleenocytes_Tcells_Vaccinated_GK15_006.fcs · Spleenocytes_Tcells_Vaccinated_GK15__2_007.fcs · Spleenocytes_Tcells_Vaccinated_GK15__3_009.fcs · Spleenocytes_Tcells_Vaccinated_Saline_004.fcs · Spleenocytes_Tcells_Vaccinated_aCD8_003.fcs |
