Experiment Overview
| Repository ID: | FR-FCM-Z6YN | Experiment name: | Exp_20220122_1_BT_RI_batch_WC_medium_2 | MIFlowCyt score: | 30.75% |
| Primary researcher: | Bin Liu | PI/manager: | Karoline Faust | Uploaded by: | Bin Liu |
| Experiment dates: | 2022-01-22 - 2022-01-28 | Dataset uploaded: | Jan 2023 | Last updated: | Jan 2024 |
| Keywords: | [live/dead staining] [human gut bacteria] [anaerobe] [model microorganism] | Manuscripts: | [37670028] | ||
| Organizations: |
KU Leuven, Leuven, Vlaams Brabant (Belgium)
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| Purpose: | Growth kinetics of monocultures Bacteroides thetaiotaomicron VPI-5482 (DSM 2079T) and Roseburia intestinalis L1-82 (DSM 14610T) and the co-culture in Wilkins-Chalgren Anaerobe Broth | ||||
| Conclusion: | None | ||||
| Comments: | live/dead cell staining followed by flow cytometry, using a benchtop CytoFLEX S flow cytometer (Beckman Coulter, Brea, USA); anaerobic cultivation of 120 h for the experiment 2 | ||||
| Funding: | H2020 European Research Council (ERC) [801747] | ||||
| Quality control: | In addition to the instrumental calibration with CytoFLEX Daily QC Fluorospheres, 0.5 μm and 1 μm green-fluorescent beads (Thermo Fisher Scientific, USA) were applied to each batch of experiments as internal standards, to monitor the instrument stability and to make cytometric data from different batches more comparable. | ||||
Experiment variables
| Conditions | |
|---|---|
| · control | 01-t04_d10_wc_blank-H3.fcs · 01-t04_d10_wc_blank-H4.fcs · 01-t08_d100_wc_blank-H5.fcs · 01-t08_d100_wc_blank-H6.fcs · 01-t0_d10_wc_blank-H1.fcs · 01-t0_d10_wc_blank-H2.fcs · 01-t12_d100_wc_blank-H7.fcs · 01-t12_d100_wc_blank-H8.fcs · 01-t16_d100_wc_blank-H10.fcs · 01-t16_d100_wc_blank-H9.fcs · 01-t20_d100_wc_blank-G1.fcs · 01-t20_d100_wc_blank-G2.fcs · 02-t24_d100_wc_blank-H1.fcs · 02-t24_d100_wc_blank-H2.fcs · 02-t28_d100_wc_blank-H3.fcs · 02-t28_d100_wc_blank-H4.fcs · 02-t32_d100_wc_blank-H5.fcs · 02-t32_d100_wc_blank-H6.fcs · 02-t36_d100_wc_blank-H7.fcs · 02-t36_d100_wc_blank-H8.fcs · 02-t40_d100_wc_blank-H10.fcs · 02-t40_d100_wc_blank-H9.fcs · 02-t44_d100_wc_blank-H11.fcs · 02-t44_d100_wc_blank-H12.fcs · 03-t120_d100_wc_blank-H10.fcs · 03-t120_d100_wc_blank-H9.fcs · 03-t48_d100_wc_blank-H1.fcs · 03-t48_d100_wc_blank-H2.fcs · 03-t60_d100_wc_blank-H3.fcs · 03-t60_d100_wc_blank-H4.fcs · 03-t72_d100_wc_blank-H5.fcs · 03-t72_d100_wc_blank-H6.fcs · 03-t96_d100_wc_blank-H7.fcs · 03-t96_d100_wc_blank-H8.fcs |
