Experiment Overview
| Repository ID: | FR-FCM-Z726 | Experiment name: | Precise analysis of single small extracellular vesicles using flow cytometry | MIFlowCyt score: | 40.36% |
| Primary researcher: | Takeshi yoshida | PI/manager: | Takeshi yoshida | Uploaded by: | Takeshi yoshida |
| Experiment dates: | 2021-04-01 - 2023-11-21 | Dataset uploaded: | Nov 2023 | Last updated: | Nov 2023 |
| Keywords: | [flow cytometry] [extracellular vesicle] [Phosphatidylserine] [sEV isolation] [sEV heterogeneity] | Manuscripts: | |||
| Organizations: |
Kanazawa University, Kanazawa, (JAPAN)
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| Purpose: | Methods that enable specific and sensitive quantification of small extracellular vesicles (sEVs) using flow cytometry are still under development. Here, we compare four preparation methods for flow cytometry, including ultracentrifugation, density gradient centrifugation, size exclusion chromatography (SEC), and the TIM4-affinity method by analyzing the specificity for antibody staining of sEVs. | ||||
| Conclusion: | SEC or the TIM4-affinity method could separate sEVs from antibodies. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
