Experiment Overview
| Repository ID: | FR-FCM-Z776 | Experiment name: | Determine the knock-in efficency of different tags inside Beta-actin | MIFlowCyt score: | 51.50% |
| Primary researcher: | Maxime van Zwam | PI/manager: | Maxime van Zwam | Uploaded by: | Maxime van Zwam |
| Experiment dates: | 2019-01-05 - 2020-11-02 | Dataset uploaded: | Feb 2024 | Last updated: | Feb 2024 |
| Keywords: | [beta-actin] [Knock-in] [oubain] | Manuscripts: | |||
| Organizations: |
Radboud University Medical centre , Department of Medical Biosciences , Nijmegen , (The Netherlands)
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| Purpose: | We want to identify the percentage of knock-in of a certain tag inside the beta-actin protein. We do this with and without co-transfection with an oubain resistence. | ||||
| Conclusion: | Doing a co-transfection with an oubain resistence gene enhance the knock-in efficiency. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | We added non transfectect parental cells as a control. | ||||
