Experiment Overview
| Repository ID: | FR-FCM-Z785 | Experiment name: | Langerhans cells | MIFlowCyt score: | 21.00% |
| Primary researcher: | Juliana Idoyaga | PI/manager: | Juliana Idoyaga | Uploaded by: | Juliana Idoyaga |
| Experiment dates: | 2024-03-04 - | Dataset uploaded: | Mar 2024 | Last updated: | Nov 2024 |
| Keywords: | None | Manuscripts: | [39541348] | ||
| Organizations: | None | ||||
| Purpose: | Monocytes give rise to Langerhans cells that preferentially migrate to lymph nodes at steady state | ||||
| Conclusion: | moLCs migrate from the skin to sLN and have a superior migratory capacity than eLCs | ||||
| Comments: | None | ||||
| Funding: | This study was supported by NIH grants to Juliana Idoyaga (AR062595, CA219994, and AR069953). CyTOF was performed on the Stanford FACs facility instrument (S10OD016318-01) | ||||
| Quality control: | For analysis, FCS files were normalized using ParkerICI/Premessa R. Cells were gated on live and singlets. Beads were removed. Lineage cells (CD3, CD19, CD335, and Ly6G) were excluded, and UMAP, PHATE, and X-shift was performed on the remaining myeloid cells using FlowJo. | ||||
