Experiment Overview
| Repository ID: | FR-FCM-Z7CD | Experiment name: | The effect of glucaric acid concentrations on the percentage of cell death | MIFlowCyt score: | 97.75% |
| Primary researcher: | nn ding | PI/manager: | nn ding | Uploaded by: | nn ding |
| Experiment dates: | 2023-12-14 - 2023-12-21 | Dataset uploaded: | Apr 2024 | Last updated: | Apr 2024 |
| Keywords: | [metabolic engineering] [glucaric acid] [transcription factor-based biosensor] [GA bioselector] [detection limit] | Manuscripts: | |||
| Organizations: |
zhejiang A&F university, State Key Laboratory of Subtropical Silviculture, hangzhou, zhejiang province (china)
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| Purpose: | To verify whether excessive GA concentration would lead to cell death. | ||||
| Conclusion: | The rate of cell death was decreased with the increase of GA concentration. | ||||
| Comments: | Performing the percentage analysis of cell death using the PI Red Dead nucleic acid staining kit by flow cytometry. | ||||
| Funding: | This work was supported by the National Natural Science Foundation of China (32301218, 31600070), the Zhejiang Provincial Natural Science Foundation of China (LZ22C200001), the Scientific Research Development Foundation of Zhejiang A&F University (2023LFR020), and the Open Project Program of State Key Laboratory of Food Science and Resources, Jiangnan University (SKLF-KF-202309). | ||||
| Quality control: | FCS collection for software testing | Remove background and adhesion | |||
Experiment variables
| Conditions | |
|---|---|
| · neg | 231221_neg_001.fcs |
| · pos | 231221_pos_002.fcs |
| · G10 | 231221_G10 100-1_007.fcs · 231221_G10 100-2_008.fcs · 231221_G10 20-1_003.fcs · 231221_G10 20-2_004.fcs · 231221_G10 50-1_005.fcs · 231221_G10 50-2_006.fcs |
| · R3 | 231221_R3 100-1_013.fcs · 231221_R3 100-2_014.fcs · 231221_R3 20-1_009.fcs · 231221_R3 20-2_010.fcs · 231221_R3 50-1_011.fcs · 231221_R3 50-2_012.fcs |
| Timepoints | |
|---|---|
| · 231221 | 231221_G10 100-1_007.fcs · 231221_G10 100-2_008.fcs · 231221_G10 20-1_003.fcs · 231221_G10 20-2_004.fcs · 231221_G10 50-1_005.fcs · 231221_G10 50-2_006.fcs · 231221_R3 100-1_013.fcs · 231221_R3 100-2_014.fcs · 231221_R3 20-1_009.fcs · 231221_R3 20-2_010.fcs · 231221_R3 50-1_011.fcs · 231221_R3 50-2_012.fcs · 231221_neg_001.fcs · 231221_pos_002.fcs |
