Experiment Overview
| Repository ID: | FR-FCM-Z7CE | Experiment name: | Flow cytometry suggests the cell-to-cell variation in GA production | MIFlowCyt score: | 97.75% |
| Primary researcher: | nn ding | PI/manager: | nn ding | Uploaded by: | nn ding |
| Experiment dates: | 2023-12-25 - 2024-01-15 | Dataset uploaded: | Apr 2024 | Last updated: | Apr 2024 |
| Keywords: | [metabolic engineering] [glucaric acid] [transcription factor-based biosensor] [GA bioselector] [detection limit] | Manuscripts: | |||
| Organizations: |
zhejiang A&F university, State Key Laboratory of Subtropical Silviculture, hangzhou, zhejiang province (china)
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| Purpose: | To evaluate whether there were cell-to-cell variations in GA-producing strains. | ||||
| Conclusion: | The strain QCGA+ had a wider sfGFP fluorescence distribution than the control strain based on the flow cytometry at 36, 48, and 60 hours. In addition, QCGA+ with Tc had higher sfGFP fluorescence intensity than QCGA+ without Tc at 36, 48, and 60 hours. It also indicated that the variation of the GA production by QCGA+ with and without Tc was 1.77-, 1.94-, and 2.15-fold at 36, 48, and 60 hours, respectively. Correspondingly, the difference in sfGFP fluorescence was also greater at 36, 48, and 60 hours. These results suggested variations between cells in the production of GA. | ||||
| Comments: | eliminating cell-to-cell variation | ||||
| Funding: | This work was supported by the National Natural Science Foundation of China (32301218, 31600070), the Zhejiang Provincial Natural Science Foundation of China (LZ22C200001), the Scientific Research Development Foundation of Zhejiang A&F University (2023LFR020), and the Open Project Program of State Key Laboratory of Food Science and Resources, Jiangnan University (SKLF-KF-202309). | ||||
| Quality control: | FCS collection for software testing | Remove background and adhesion | |||
Experiment variables
| Conditions | |
|---|---|
| · -Tc | 240108_G10-MIU-Tc-1-60_023.fcs · 240108_G10-MIU-Tc-2-60_024.fcs · 240108_R3-MIU-Tc-1-36_027.fcs · 240108_R3-MIU-Tc-1-48_031.fcs · 240108_R3-MIU-Tc-1-60_035.fcs · 240108_R3-MIU-Tc-2-36_028.fcs · 240108_R3-MIU-Tc-2-48_032.fcs · 240108_R3-MIU-Tc-2-60_036.fcs |
| · +Tc | 240108_G10-MIU+Tc-1-60_025.fcs · 240108_G10-MIU+Tc-2-60_026.fcs · 240108_R3-MIU+Tc-1-36_029.fcs · 240108_R3-MIU+Tc-1-48_033.fcs · 240108_R3-MIU+Tc-1-60_037.fcs · 240108_R3-MIU+Tc-2-36_030.fcs · 240108_R3-MIU+Tc-2-48_034.fcs · 240108_R3-MIU+Tc-2-60_038.fcs |
| Sample Type | |
|---|---|
| · G10 | 240108_G10-MIU+Tc-1-60_025.fcs · 240108_G10-MIU+Tc-2-60_026.fcs · 240108_G10-MIU-Tc-1-60_023.fcs · 240108_G10-MIU-Tc-2-60_024.fcs |
| · R3 | 240108_R3-MIU+Tc-1-36_029.fcs · 240108_R3-MIU+Tc-1-48_033.fcs · 240108_R3-MIU+Tc-1-60_037.fcs · 240108_R3-MIU+Tc-2-36_030.fcs · 240108_R3-MIU+Tc-2-48_034.fcs · 240108_R3-MIU+Tc-2-60_038.fcs · 240108_R3-MIU-Tc-1-36_027.fcs · 240108_R3-MIU-Tc-1-48_031.fcs · 240108_R3-MIU-Tc-1-60_035.fcs · 240108_R3-MIU-Tc-2-36_028.fcs · 240108_R3-MIU-Tc-2-48_032.fcs · 240108_R3-MIU-Tc-2-60_036.fcs |
| · MSD6 | 240108_MSD6-1-36_003.fcs · 240108_MSD6-1-48_010.fcs · 240108_MSD6-1-60_004.fcs · 240108_MSD6-2-36_012.fcs · 240108_MSD6-2-48_011.fcs · 240108_MSD6-2-60_005.fcs |
| Timepoints | |
|---|---|
| · 36 | 240108_MSD6-1-36_003.fcs · 240108_MSD6-2-36_012.fcs · 240108_R3-MIU+Tc-1-36_029.fcs · 240108_R3-MIU+Tc-2-36_030.fcs · 240108_R3-MIU-Tc-1-36_027.fcs · 240108_R3-MIU-Tc-2-36_028.fcs · 240108_R3-MIU-Tc-2-60_036.fcs |
| · 48 | 240108_MSD6-1-48_010.fcs · 240108_MSD6-2-48_011.fcs · 240108_R3-MIU+Tc-1-48_033.fcs · 240108_R3-MIU+Tc-2-48_034.fcs · 240108_R3-MIU-Tc-1-48_031.fcs · 240108_R3-MIU-Tc-2-48_032.fcs |
| · 60 | 240108_G10-MIU+Tc-1-60_025.fcs · 240108_G10-MIU+Tc-2-60_026.fcs · 240108_G10-MIU-Tc-1-60_023.fcs · 240108_G10-MIU-Tc-2-60_024.fcs · 240108_MSD6-1-60_004.fcs · 240108_MSD6-2-60_005.fcs · 240108_R3-MIU+Tc-1-60_037.fcs · 240108_R3-MIU+Tc-2-60_038.fcs · 240108_R3-MIU-Tc-1-60_035.fcs |
