Experiment Overview
| Repository ID: | FR-FCM-Z7D4 | Experiment name: | Ronapreve_Bcell data | MIFlowCyt score: | 69.47% |
| Primary researcher: | Axel Schulz | PI/manager: | Axel Schulz | Uploaded by: | Axel Schulz |
| Experiment dates: | 2022-01-01 - 2022-06-30 | Dataset uploaded: | Apr 2024 | Last updated: | Apr 2024 |
| Keywords: | [COVID-19] [SARS-CoV-2] [mRNA Vaccination] [Ronapreve] [Casirivimab] [Imdevimab] [active vaccination] [passive immunization] [neutralization] [rituximab] [immunocompromised patients] [non-responders] [antibody feedback] | Manuscripts: | |||
| Organizations: |
German Rheumatism Research Center Berlin, a Leibniz-Institute (DRFZ), Immunemonitoring, Berlin, (Germany)
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| Purpose: | Active and passive immunization is used in high-risk patients to prevent severe courses of COVID-19, but the impact of prophylactic neutralizing antibodies on the immune reaction to the mRNA vaccines has remained enigmatic. | ||||
| Conclusion: | Here we show that CD4 T and B cell responses to Spikevax booster immunization are suppressed by the therapeutic antibodies Casirivimab and Imdevimab. B cell and T cell responses were significantly induced in controls but not in antibody-treated patients. The data indicates that humoral immunity, i. e. high levels of antibodies, negatively impacts reactive immunity, resulting in blunted cellular responses upon boosting. This argues for temporal separation of vaccination efforts; with active vaccination preferably applied before prophylactic therapeutic antibody treatment. | ||||
| Comments: | Gated data of Bcells and plasmablasts (see Gating strategy), exported from OMIQ.ai | ||||
| Funding: | The study was supported by grants TRR130 TP24 (DFG), funding by the Berlin Senate, and the European Union Innovative Medicines Initiative 2 Joint Undertaking under grant agreements no. 777357 (RTCure) and no. 831434 (3TR). | ||||
| Quality control: | compensation with single stained controls | ||||
Experiment variables
| Individuals | |
|---|---|
| · R005 | Bcells_A1_R005_d0_2022-03-03.0001.mqd.fcs · Bcells_B1_R005_d7_2022-03-03.0001.mqd.fcs · Bcells_C1_R005_d28_2022-03-03.0001.mqd.fcs |
| · R007 | Bcells_A1_R007_d0_2022-03-04.0001.mqd.fcs · Bcells_B1_R007_d7_2022-03-04.0001.mqd.fcs |
| · R010 | Bcells_A2_R010_d0_2022-03-04.0001.mqd.fcs · Bcells_B2_R010_d7_2022-03-04.0001.mqd.fcs · Bcells_C2_R010_d28_2022-03-04.0001.mqd.fcs |
| · R011 | Bcells_A2_R011_d28_2022-03-03.0001.mqd.fcs · Bcells_G1_R011_d0_2022-03-03.0001.mqd.fcs · Bcells_H1_R011_d7_2022-03-03.0001.mqd.fcs |
| · R015 | Bcells_A3_R015_d7_2022-03-03.0001.mqd.fcs · Bcells_B3_R015_d28_2022-03-03.0001.mqd.fcs · Bcells_H2_R015_d0_2022-03-03.0001.mqd.fcs |
| · R016 | Bcells_A3_R016_d28_2022-03-04.0001.mqd.fcs · Bcells_G2_R016_d0_2022-03-04.0001.mqd.fcs · Bcells_H2_R016_d7_2022-03-04.0001.mqd.fcs |
| · R021 | Bcells_A4_R021_d28_2022-03-04.0001.mqd.fcs · Bcells_G3_R021_d0_2022-03-04.0001.mqd.fcs · Bcells_H3_R021_d7_2022-03-04.0001.mqd.fcs |
| · R012 | Bcells_B2_R012_d0_2022-03-03.0001.mqd.fcs · Bcells_C2_R012_d7_2022-03-03.0001.mqd.fcs · Bcells_D2_R012_d28_2022-03-03.0001.mqd.fcs |
| · R017 | Bcells_B3_R017_d0_2022-03-04.0001.mqd.fcs · Bcells_C3_R017_d7_2022-03-04.0001.mqd.fcs · Bcells_D3_R017_d28_2022-03-04.0001.mqd.fcs |
| · R008 | Bcells_C1_R008_d0_2022-03-04.0001.mqd.fcs · Bcells_D1_R008_d7_2022-03-04.0001.mqd.fcs · Bcells_E1_R008_d28_2022-03-04.0001.mqd.fcs |
| · R013 | Bcells_D2_R013_d0_2022-03-04.0001.mqd.fcs · Bcells_E2_R013_d7_2022-03-04.0001.mqd.fcs · Bcells_F2_R013_d28_2022-03-04.0001.mqd.fcs |
| · R019 | Bcells_C3_R019_d0_2022-03-03.0001.mqd.fcs · Bcells_D3_R019_d7_2022-03-03.0001.mqd.fcs · Bcells_E3_R019_d28_2022-03-03.0001.mqd.fcs |
| · R006 | Bcells_D1_R006_d0_2022-03-03.0001.mqd.fcs · Bcells_E1_R006_d7_2022-03-03.0001.mqd.fcs · Bcells_F1_R006_d28_2022-03-03.0001.mqd.fcs |
| · R020 | Bcells_F3_R020_d0_2022-03-03.0001.mqd.fcs · Bcells_G3_R020_d7_2022-03-03.0001.mqd.fcs · Bcells_H3_R020_d28_2022-03-03.0001.mqd.fcs |
| · R014 | Bcells_E2_R014_d0_2022-03-03.0001.mqd.fcs · Bcells_F2_R014_d7_2022-03-03.0001.mqd.fcs · Bcells_G2_R014_d28_2022-03-03.0001.mqd.fcs |
| · R009 | Bcells_F1_R009_d0_2022-03-04.0001.mqd.fcs · Bcells_G1_R009_d7_2022-03-04.0001.mqd.fcs · Bcells_H1_R009_d28_2022-03-04.0001.mqd.fcs |
| · R018 | Bcells_E3_R018_d0_2022-03-04.0001.mqd.fcs · Bcells_F3_R018_d28_2022-03-04.0001.mqd.fcs |
| Timepoints | |
|---|---|
| · d0 | Bcells_A1_R005_d0_2022-03-03.0001.mqd.fcs · Bcells_A1_R007_d0_2022-03-04.0001.mqd.fcs · Bcells_A2_R010_d0_2022-03-04.0001.mqd.fcs · Bcells_B2_R012_d0_2022-03-03.0001.mqd.fcs · Bcells_B3_R017_d0_2022-03-04.0001.mqd.fcs · Bcells_C1_R008_d0_2022-03-04.0001.mqd.fcs · Bcells_C3_R019_d0_2022-03-03.0001.mqd.fcs · Bcells_D1_R006_d0_2022-03-03.0001.mqd.fcs · Bcells_D2_R013_d0_2022-03-04.0001.mqd.fcs · Bcells_E2_R014_d0_2022-03-03.0001.mqd.fcs · Bcells_E3_R018_d0_2022-03-04.0001.mqd.fcs · Bcells_F1_R009_d0_2022-03-04.0001.mqd.fcs · Bcells_F3_R020_d0_2022-03-03.0001.mqd.fcs · Bcells_G1_R011_d0_2022-03-03.0001.mqd.fcs · Bcells_G2_R016_d0_2022-03-04.0001.mqd.fcs · Bcells_G3_R021_d0_2022-03-04.0001.mqd.fcs · Bcells_H2_R015_d0_2022-03-03.0001.mqd.fcs |
| · d7 | Bcells_A3_R015_d7_2022-03-03.0001.mqd.fcs · Bcells_B1_R005_d7_2022-03-03.0001.mqd.fcs · Bcells_B1_R007_d7_2022-03-04.0001.mqd.fcs · Bcells_B2_R010_d7_2022-03-04.0001.mqd.fcs · Bcells_C2_R012_d7_2022-03-03.0001.mqd.fcs · Bcells_C3_R017_d7_2022-03-04.0001.mqd.fcs · Bcells_D1_R008_d7_2022-03-04.0001.mqd.fcs · Bcells_D3_R019_d7_2022-03-03.0001.mqd.fcs · Bcells_E1_R006_d7_2022-03-03.0001.mqd.fcs · Bcells_E2_R013_d7_2022-03-04.0001.mqd.fcs · Bcells_F2_R014_d7_2022-03-03.0001.mqd.fcs · Bcells_G1_R009_d7_2022-03-04.0001.mqd.fcs · Bcells_G3_R020_d7_2022-03-03.0001.mqd.fcs · Bcells_H1_R011_d7_2022-03-03.0001.mqd.fcs · Bcells_H2_R016_d7_2022-03-04.0001.mqd.fcs · Bcells_H3_R021_d7_2022-03-04.0001.mqd.fcs |
| · d28 | Bcells_A2_R011_d28_2022-03-03.0001.mqd.fcs · Bcells_A3_R016_d28_2022-03-04.0001.mqd.fcs · Bcells_A4_R021_d28_2022-03-04.0001.mqd.fcs · Bcells_B3_R015_d28_2022-03-03.0001.mqd.fcs · Bcells_C1_R005_d28_2022-03-03.0001.mqd.fcs · Bcells_C2_R010_d28_2022-03-04.0001.mqd.fcs · Bcells_D2_R012_d28_2022-03-03.0001.mqd.fcs · Bcells_D3_R017_d28_2022-03-04.0001.mqd.fcs · Bcells_E1_R008_d28_2022-03-04.0001.mqd.fcs · Bcells_E3_R019_d28_2022-03-03.0001.mqd.fcs · Bcells_F1_R006_d28_2022-03-03.0001.mqd.fcs · Bcells_F2_R013_d28_2022-03-04.0001.mqd.fcs · Bcells_F3_R018_d28_2022-03-04.0001.mqd.fcs · Bcells_G2_R014_d28_2022-03-03.0001.mqd.fcs · Bcells_H1_R009_d28_2022-03-04.0001.mqd.fcs · Bcells_H3_R020_d28_2022-03-03.0001.mqd.fcs |
