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Experiment Overview

Repository ID: FR-FCM-Z7DA Experiment name: Ronapreve_Tcell data MIFlowCyt score: 72.00%
Primary researcher: Axel Schulz PI/manager: Axel Schulz Uploaded by: Axel Schulz
Experiment dates: 2022-01-01 - 2022-06-30 Dataset uploaded: Apr 2024 Last updated: Apr 2024
Keywords: [COVID-19] [SARS-CoV-2] [mRNA Vaccination] [Ronapreve] [Casirivimab] [Imdevimab] [active vaccination] [passive immunization] [neutralization] [rituximab] [immunocompromised patients] [non-responders] [antibody feedback] Manuscripts:
Organizations: German Rheumatism Research Center Berlin, a Leibniz-Institute (DRFZ), Immunemonitoring, Berlin, (Germany)
Purpose: Active and passive immunization is used in high-risk patients to prevent severe courses of COVID-19, but the impact of prophylactic neutralizing antibodies on the immune reaction to the mRNA vaccines has remained enigmatic.
Conclusion: Here we show that CD4 T and B cell responses to Spikevax booster immunization are suppressed by the therapeutic antibodies Casirivimab and Imdevimab. B cell and T cell responses were significantly induced in controls but not in antibody-treated patients. The data indicates that humoral immunity, i. e. high levels of antibodies, negatively impacts reactive immunity, resulting in blunted cellular responses upon boosting. This argues for temporal separation of vaccination efforts; with active vaccination preferably applied before prophylactic therapeutic antibody treatment.
Comments: None
Funding: The study was supported by grants TRR130 TP24 (DFG), funding by the Berlin Senate, and the European Union Innovative Medicines Initiative 2 Joint Undertaking under grant agreements no. 777357 (RTCure) and no. 831434 (3TR).
Quality control: Clean-up FlowAI Gated data of Bcells and plasmablasts (see Gating strategy), exported from OMIQ.ai


Experiment variables

Timepoints
· d0 CD4_A1_d0_unstim_R005_2022-01-05.0001.mqd_R005_Run1_unstim.fcs · CD4_A1_d0_unstim_R016_2022-01-17.0001.mqd_R016_Run2_unstim.fcs · CD4_A2_d0_Spike_R018_2022-01-17.0001.mqd_R018_Run2_Spike.fcs · CD4_A2_d0_unstim_R013_2022-01-05.0001.mqd_R013_Run1_unstim.fcs · CD4_A3_d0_SEB_R020_2022-01-17.0001.mqd_R020_Run2_SEB.fcs · CD4_A3_d0_Spike_R010_2022-01-05.0001.mqd_R010_Run1_Spike.fcs · CD4_A4_d0_SEB_R007_2022-01-05.0001.mqd_R007_Run1_SEB.fcs · CD4_A5_d0_SEB_R015_2022-01-05.0001.mqd_R015_Run1_SEB.fcs · CD4_B1_d0_unstim_R006_2022-01-05.0001.mqd_R006_Run1_unstim.fcs · CD4_B1_d0_unstim_R017_2022-01-17.0001.mqd_R017_Run2_unstim.fcs · CD4_B2_d0_Spike_R019_2022-01-17.0001.mqd_R019_Run2_Spike.fcs · CD4_B2_d0_unstim_R014_2022-01-05.0001.mqd_R014_Run1_unstim.fcs · CD4_B3_d0_SEB_R021_2022-01-17.0001.mqd_R021_Run2_SEB.fcs · CD4_B3_d0_Spike_R011_2022-01-05.0001.mqd_R011_Run1_Spike.fcs · CD4_B4_d0_SEB_R008_2022-01-05.0001.mqd_R008_Run1_SEB.fcs · CD4_C1_d0_unstim_R007_2022-01-05.0001.mqd_R007_Run1_unstim.fcs · CD4_C1_d0_unstim_R018_2022-01-17.0001.mqd_R018_Run2_unstim.fcs · CD4_C2_d0_Spike_R020_2022-01-17.0001.mqd_R020_Run2_Spike.fcs · CD4_C2_d0_unstim_R015_2022-01-05.0001.mqd_R015_Run1_unstim.fcs · CD4_C3_d0_Spike_R012_2022-01-05.0001.mqd_R012_Run1_Spike.fcs · CD4_C4_d0_SEB_R009_2022-01-05.0001.mqd_R009_Run1_SEB.fcs · CD4_D1_d0_unstim_R008_2022-01-05.0001.mqd_R008_Run1_unstim.fcs · CD4_D1_d0_unstim_R019_2022-01-17.0001.mqd_R019_Run2_unstim.fcs · CD4_D2_d0_Spike_R005_2022-01-05.0001.mqd_R005_Run1_Spike.fcs · CD4_D2_d0_Spike_R021_2022-01-17.0001.mqd_R021_Run2_Spike.fcs · CD4_D3_d0_Spike_R013_2022-01-05.0001.mqd_R013_Run1_Spike.fcs · CD4_D4_d0_SEB_R010_2022-01-05.0001.mqd_R010_Run1_SEB.fcs · CD4_E1_d0_unstim_R009_2022-01-05.0001.mqd_R009_Run1_unstim.fcs · CD4_E1_d0_unstim_R020_2022-01-17.0001.mqd_R020_Run2_unstim.fcs · CD4_E2_d0_SEB_R016_2022-01-17.0001.mqd_R016_Run2_SEB.fcs · CD4_E2_d0_Spike_R006_2022-01-05.0001.mqd_R006_Run1_Spike.fcs · CD4_E3_d0_Spike_R014_2022-01-05.0001.mqd_R014_Run1_Spike.fcs · CD4_E4_d0_SEB_R011_2022-01-05.0001.mqd_R011_Run1_SEB.fcs · CD4_F1_d0_unstim_R010_2022-01-05.0001.mqd_R010_Run1_unstim.fcs · CD4_F1_d0_unstim_R021_2022-01-17.0001.mqd_R021_Run2_unstim.fcs · CD4_F2_d0_SEB_R017_2022-01-17.0001.mqd_R017_Run2_SEB.fcs · CD4_F2_d0_Spike_R007_2022-01-05.0001.mqd_R007_Run1_Spike.fcs · CD4_F3_d0_Spike_R015_2022-01-05.0001.mqd_R015_Run1_Spike.fcs · CD4_F4_d0_SEB_R012_2022-01-05.0001.mqd_R012_Run1_SEB.fcs · CD4_G1_d0_Spike_R016_2022-01-17.0001.mqd_R016_Run2_Spike.fcs · CD4_G1_d0_unstim_R011_2022-01-05.0001.mqd_R011_Run1_unstim.fcs · CD4_G2_d0_SEB_R018_2022-01-17.0001.mqd_R018_Run2_SEB.fcs · CD4_G2_d0_Spike_R008_2022-01-05.0001.mqd_R008_Run1_Spike.fcs · CD4_G3_d0_SEB_R005_2022-01-05.0001.mqd_R005_Run1_SEB.fcs · CD4_G4_d0_SEB_R013_2022-01-05.0001.mqd_R013_Run1_SEB.fcs · CD4_H1_d0_Spike_R017_2022-01-17.0001.mqd_R017_Run2_Spike.fcs · CD4_H1_d0_unstim_R012_2022-01-05.0001.mqd_R012_Run1_unstim.fcs · CD4_H2_d0_SEB_R019_2022-01-17.0001.mqd_R019_Run2_SEB.fcs · CD4_H2_d0_Spike_R009_2022-01-05.0001.mqd_R009_Run1_Spike.fcs · CD4_H3_d0_SEB_R006_2022-01-05.0001.mqd_R006_Run1_SEB.fcs · CD4_H4_d0_SEB_R014_2022-01-05.0001.mqd_R014_Run1_SEB.fcs
· d28 CD4_A1_d28_unstim_R005_2022-02-03.0001.mqd_R005_Run3_unstim.fcs · CD4_A1_d28_unstim_R016_2022-02-14.0001.mqd_R016_run4_unstim.fcs · CD4_A2_d28_Spike_R005_2022-02-03.0001.mqd_R005_Run3_Spike.fcs · CD4_A2_d28_Spike_R018_2022-02-14.0001.mqd_R018_run4_Spike.fcs · CD4_A3_d28_SEB_R005_2022-02-03.0001.mqd_R005_Run3_SEB.fcs · CD4_A3_d28_SEB_R020_2022-02-14.0001.mqd_R020_run4_SEB.fcs · CD4_A4_d28_unstim_R011_2022-02-03.0001.mqd_R011_Run3_unstim.fcs · CD4_B1_d27_unstim_R017_2022-02-14.0001.mqd_R017_run4_unstim.fcs · CD4_B1_d28_unstim_R006_2022-02-03.0001.mqd_R006_Run3_unstim.fcs · CD4_B2_d28_Spike_R006_2022-02-03.0001.mqd_R006_Run3_Spike.fcs · CD4_B2_d28_Spike_R019_2022-02-14.0001.mqd_R019_run4_Spike.fcs · CD4_B3_d28_SEB_R006_2022-02-03.0001.mqd_R006_Run3_SEB.fcs · CD4_B3_d28_SEB_R021_2022-02-14.0001.mqd_R021_run4_SEB.fcs · CD4_B4_d28_Spike_R011_2022-02-03.0001.mqd_R011_Run3_Spike.fcs · CD4_C1_d28_unstim_R008_2022-02-03.0001.mqd_R008_Run3_unstim.fcs · CD4_C1_d28_unstim_R018_2022-02-14.0001.mqd_R018_run4_unstim.fcs · CD4_C2_d28_Spike_R008_2022-02-03.0001.mqd_R008_Run3_Spike.fcs · CD4_C2_d28_Spike_R020_2022-02-14.0001.mqd_R020_run4_Spike.fcs · CD4_C3_d28_SEB_R008_2022-02-03.0001.mqd_R008_Run3_SEB.fcs · CD4_C4_d28_SEB_R011_2022-02-03.0001.mqd_R011_Run3_SEB.fcs · CD4_D1_d28_unstim_R009_2022-02-03.0001.mqd_R009_Run3_unstim.fcs · CD4_D1_d28_unstim_R019_2022-02-14.0001.mqd_R019_run4_unstim.fcs · CD4_D2_d28_Spike_R009_2022-02-03.0001.mqd_R009_Run3_Spike.fcs · CD4_D2_d28_Spike_R021_2022-02-14.0001.mqd_R021_run4_Spike.fcs · CD4_D3_d28_SEB_R009_2022-02-03.0001.mqd_R009_Run3_SEB.fcs · CD4_E1_d28_unstim_R010_2022-02-03.0001.mqd_R010_Run3_unstim.fcs · CD4_E1_d28_unstim_R020_2022-02-14.0001.mqd_R020_run4_unstim.fcs · CD4_E2_d28_SEB_R016_2022-02-14.0001.mqd_R016_run4_SEB.fcs · CD4_E2_d28_Spike_R010_2022-02-03.0001.mqd_R010_Run3_Spike.fcs · CD4_E3_d28_SEB_R010_2022-02-03.0001.mqd_R010_Run3_SEB.fcs · CD4_F1_d28_unstim_R012_2022-02-03.0001.mqd_R012_Run3_unstim.fcs · CD4_F1_d28_unstim_R021_2022-02-14.0001.mqd_R021_run4_unstim.fcs · CD4_F2_d27_SEB_R017_2022-02-14.0001.mqd_R017_run4_SEB.fcs · CD4_F2_d28_Spike_R012_2022-02-03.0001.mqd_R012_Run3_Spike.fcs · CD4_F3_d28_SEB_R012_2022-02-03.0001.mqd_R012_Run3_SEB.fcs · CD4_G1_d28_Spike_R016_2022-02-14.0001.mqd_R016_run4_Spike.fcs · CD4_G1_d28_unstim_R013_2022-02-03.0001.mqd_R013_Run3_unstim.fcs · CD4_G2_d28_SEB_R018_2022-02-14.0001.mqd_R018_run4_SEB.fcs · CD4_G2_d28_Spike_R013_2022-02-03.0001.mqd_R013_Run3_Spike.fcs · CD4_G3_d28_SEB_R013_2022-02-03.0001.mqd_R013_Run3_SEB.fcs · CD4_H1_d27_Spike_R017_2022-02-14.0001.mqd_R017_run4_Spike.fcs · CD4_H1_d28_unstim_R015_2022-02-03.0001.mqd_R015_Run3_unstim.fcs · CD4_H2_d28_SEB_R019_2022-02-14.0001.mqd_R019_run4_SEB.fcs · CD4_H2_d28_Spike_R015_2022-02-03.0001.mqd_R015_Run3_Spike.fcs · CD4_H3_d28_SEB_R015_2022-02-03.0001.mqd_R015_Run3_SEB.fcs

Individuals
· R005 CD4_A1_d0_unstim_R005_2022-01-05.0001.mqd_R005_Run1_unstim.fcs · CD4_A1_d28_unstim_R005_2022-02-03.0001.mqd_R005_Run3_unstim.fcs · CD4_A2_d28_Spike_R005_2022-02-03.0001.mqd_R005_Run3_Spike.fcs · CD4_A3_d28_SEB_R005_2022-02-03.0001.mqd_R005_Run3_SEB.fcs · CD4_D2_d0_Spike_R005_2022-01-05.0001.mqd_R005_Run1_Spike.fcs · CD4_G3_d0_SEB_R005_2022-01-05.0001.mqd_R005_Run1_SEB.fcs
· R016 CD4_A1_d0_unstim_R016_2022-01-17.0001.mqd_R016_Run2_unstim.fcs · CD4_A1_d28_unstim_R016_2022-02-14.0001.mqd_R016_run4_unstim.fcs · CD4_E2_d0_SEB_R016_2022-01-17.0001.mqd_R016_Run2_SEB.fcs · CD4_E2_d28_SEB_R016_2022-02-14.0001.mqd_R016_run4_SEB.fcs · CD4_G1_d0_Spike_R016_2022-01-17.0001.mqd_R016_Run2_Spike.fcs · CD4_G1_d28_Spike_R016_2022-02-14.0001.mqd_R016_run4_Spike.fcs
· R018 CD4_A2_d0_Spike_R018_2022-01-17.0001.mqd_R018_Run2_Spike.fcs · CD4_A2_d28_Spike_R018_2022-02-14.0001.mqd_R018_run4_Spike.fcs · CD4_C1_d0_unstim_R018_2022-01-17.0001.mqd_R018_Run2_unstim.fcs · CD4_C1_d28_unstim_R018_2022-02-14.0001.mqd_R018_run4_unstim.fcs · CD4_G2_d0_SEB_R018_2022-01-17.0001.mqd_R018_Run2_SEB.fcs · CD4_G2_d28_SEB_R018_2022-02-14.0001.mqd_R018_run4_SEB.fcs
· R020 CD4_A3_d0_SEB_R020_2022-01-17.0001.mqd_R020_Run2_SEB.fcs · CD4_A3_d28_SEB_R020_2022-02-14.0001.mqd_R020_run4_SEB.fcs · CD4_C2_d0_Spike_R020_2022-01-17.0001.mqd_R020_Run2_Spike.fcs · CD4_C2_d28_Spike_R020_2022-02-14.0001.mqd_R020_run4_Spike.fcs · CD4_E1_d0_unstim_R020_2022-01-17.0001.mqd_R020_Run2_unstim.fcs · CD4_E1_d28_unstim_R020_2022-02-14.0001.mqd_R020_run4_unstim.fcs
· R010 CD4_A3_d0_Spike_R010_2022-01-05.0001.mqd_R010_Run1_Spike.fcs · CD4_D4_d0_SEB_R010_2022-01-05.0001.mqd_R010_Run1_SEB.fcs · CD4_E1_d28_unstim_R010_2022-02-03.0001.mqd_R010_Run3_unstim.fcs · CD4_E2_d28_Spike_R010_2022-02-03.0001.mqd_R010_Run3_Spike.fcs · CD4_E3_d28_SEB_R010_2022-02-03.0001.mqd_R010_Run3_SEB.fcs · CD4_F1_d0_unstim_R010_2022-01-05.0001.mqd_R010_Run1_unstim.fcs
· R015 CD4_A5_d0_SEB_R015_2022-01-05.0001.mqd_R015_Run1_SEB.fcs · CD4_C2_d0_unstim_R015_2022-01-05.0001.mqd_R015_Run1_unstim.fcs · CD4_F3_d0_Spike_R015_2022-01-05.0001.mqd_R015_Run1_Spike.fcs · CD4_H1_d28_unstim_R015_2022-02-03.0001.mqd_R015_Run3_unstim.fcs · CD4_H2_d28_Spike_R015_2022-02-03.0001.mqd_R015_Run3_Spike.fcs · CD4_H3_d28_SEB_R015_2022-02-03.0001.mqd_R015_Run3_SEB.fcs
· R017 CD4_B1_d0_unstim_R017_2022-01-17.0001.mqd_R017_Run2_unstim.fcs · CD4_B1_d27_unstim_R017_2022-02-14.0001.mqd_R017_run4_unstim.fcs · CD4_F2_d0_SEB_R017_2022-01-17.0001.mqd_R017_Run2_SEB.fcs · CD4_F2_d27_SEB_R017_2022-02-14.0001.mqd_R017_run4_SEB.fcs · CD4_H1_d0_Spike_R017_2022-01-17.0001.mqd_R017_Run2_Spike.fcs · CD4_H1_d27_Spike_R017_2022-02-14.0001.mqd_R017_run4_Spike.fcs
· R019 CD4_B2_d0_Spike_R019_2022-01-17.0001.mqd_R019_Run2_Spike.fcs · CD4_B2_d28_Spike_R019_2022-02-14.0001.mqd_R019_run4_Spike.fcs · CD4_D1_d0_unstim_R019_2022-01-17.0001.mqd_R019_Run2_unstim.fcs · CD4_D1_d28_unstim_R019_2022-02-14.0001.mqd_R019_run4_unstim.fcs · CD4_H2_d0_SEB_R019_2022-01-17.0001.mqd_R019_Run2_SEB.fcs · CD4_H2_d28_SEB_R019_2022-02-14.0001.mqd_R019_run4_SEB.fcs
· R013 CD4_A2_d0_unstim_R013_2022-01-05.0001.mqd_R013_Run1_unstim.fcs · CD4_D3_d0_Spike_R013_2022-01-05.0001.mqd_R013_Run1_Spike.fcs · CD4_G1_d28_unstim_R013_2022-02-03.0001.mqd_R013_Run3_unstim.fcs · CD4_G2_d28_Spike_R013_2022-02-03.0001.mqd_R013_Run3_Spike.fcs · CD4_G3_d28_SEB_R013_2022-02-03.0001.mqd_R013_Run3_SEB.fcs · CD4_G4_d0_SEB_R013_2022-01-05.0001.mqd_R013_Run1_SEB.fcs
· R007 CD4_A4_d0_SEB_R007_2022-01-05.0001.mqd_R007_Run1_SEB.fcs · CD4_C1_d0_unstim_R007_2022-01-05.0001.mqd_R007_Run1_unstim.fcs · CD4_F2_d0_Spike_R007_2022-01-05.0001.mqd_R007_Run1_Spike.fcs
· R006 CD4_B1_d0_unstim_R006_2022-01-05.0001.mqd_R006_Run1_unstim.fcs · CD4_B1_d28_unstim_R006_2022-02-03.0001.mqd_R006_Run3_unstim.fcs · CD4_B2_d28_Spike_R006_2022-02-03.0001.mqd_R006_Run3_Spike.fcs · CD4_B3_d28_SEB_R006_2022-02-03.0001.mqd_R006_Run3_SEB.fcs · CD4_E2_d0_Spike_R006_2022-01-05.0001.mqd_R006_Run1_Spike.fcs · CD4_H3_d0_SEB_R006_2022-01-05.0001.mqd_R006_Run1_SEB.fcs
· R011 CD4_A4_d28_unstim_R011_2022-02-03.0001.mqd_R011_Run3_unstim.fcs · CD4_B3_d0_Spike_R011_2022-01-05.0001.mqd_R011_Run1_Spike.fcs · CD4_B4_d28_Spike_R011_2022-02-03.0001.mqd_R011_Run3_Spike.fcs · CD4_C4_d28_SEB_R011_2022-02-03.0001.mqd_R011_Run3_SEB.fcs · CD4_E4_d0_SEB_R011_2022-01-05.0001.mqd_R011_Run1_SEB.fcs · CD4_G1_d0_unstim_R011_2022-01-05.0001.mqd_R011_Run1_unstim.fcs
· R014 CD4_B2_d0_unstim_R014_2022-01-05.0001.mqd_R014_Run1_unstim.fcs · CD4_E3_d0_Spike_R014_2022-01-05.0001.mqd_R014_Run1_Spike.fcs · CD4_H4_d0_SEB_R014_2022-01-05.0001.mqd_R014_Run1_SEB.fcs
· R021 CD4_B3_d0_SEB_R021_2022-01-17.0001.mqd_R021_Run2_SEB.fcs · CD4_B3_d28_SEB_R021_2022-02-14.0001.mqd_R021_run4_SEB.fcs · CD4_D2_d0_Spike_R021_2022-01-17.0001.mqd_R021_Run2_Spike.fcs · CD4_D2_d28_Spike_R021_2022-02-14.0001.mqd_R021_run4_Spike.fcs · CD4_F1_d0_unstim_R021_2022-01-17.0001.mqd_R021_Run2_unstim.fcs · CD4_F1_d28_unstim_R021_2022-02-14.0001.mqd_R021_run4_unstim.fcs
· R008 CD4_B4_d0_SEB_R008_2022-01-05.0001.mqd_R008_Run1_SEB.fcs · CD4_C1_d28_unstim_R008_2022-02-03.0001.mqd_R008_Run3_unstim.fcs · CD4_C2_d28_Spike_R008_2022-02-03.0001.mqd_R008_Run3_Spike.fcs · CD4_C3_d28_SEB_R008_2022-02-03.0001.mqd_R008_Run3_SEB.fcs · CD4_D1_d0_unstim_R008_2022-01-05.0001.mqd_R008_Run1_unstim.fcs · CD4_G2_d0_Spike_R008_2022-01-05.0001.mqd_R008_Run1_Spike.fcs
· R012 CD4_C3_d0_Spike_R012_2022-01-05.0001.mqd_R012_Run1_Spike.fcs · CD4_F1_d28_unstim_R012_2022-02-03.0001.mqd_R012_Run3_unstim.fcs · CD4_F2_d28_Spike_R012_2022-02-03.0001.mqd_R012_Run3_Spike.fcs · CD4_F3_d28_SEB_R012_2022-02-03.0001.mqd_R012_Run3_SEB.fcs · CD4_F4_d0_SEB_R012_2022-01-05.0001.mqd_R012_Run1_SEB.fcs · CD4_H1_d0_unstim_R012_2022-01-05.0001.mqd_R012_Run1_unstim.fcs
· R009 CD4_C4_d0_SEB_R009_2022-01-05.0001.mqd_R009_Run1_SEB.fcs · CD4_D1_d28_unstim_R009_2022-02-03.0001.mqd_R009_Run3_unstim.fcs · CD4_D2_d28_Spike_R009_2022-02-03.0001.mqd_R009_Run3_Spike.fcs · CD4_D3_d28_SEB_R009_2022-02-03.0001.mqd_R009_Run3_SEB.fcs · CD4_E1_d0_unstim_R009_2022-01-05.0001.mqd_R009_Run1_unstim.fcs · CD4_H2_d0_Spike_R009_2022-01-05.0001.mqd_R009_Run1_Spike.fcs

Conditions
· unstim CD4_A1_d0_unstim_R005_2022-01-05.0001.mqd_R005_Run1_unstim.fcs · CD4_A1_d0_unstim_R016_2022-01-17.0001.mqd_R016_Run2_unstim.fcs · CD4_A1_d28_unstim_R005_2022-02-03.0001.mqd_R005_Run3_unstim.fcs · CD4_A1_d28_unstim_R016_2022-02-14.0001.mqd_R016_run4_unstim.fcs · CD4_A2_d0_unstim_R013_2022-01-05.0001.mqd_R013_Run1_unstim.fcs · CD4_A4_d28_unstim_R011_2022-02-03.0001.mqd_R011_Run3_unstim.fcs · CD4_B1_d0_unstim_R006_2022-01-05.0001.mqd_R006_Run1_unstim.fcs · CD4_B1_d0_unstim_R017_2022-01-17.0001.mqd_R017_Run2_unstim.fcs · CD4_B1_d27_unstim_R017_2022-02-14.0001.mqd_R017_run4_unstim.fcs · CD4_B1_d28_unstim_R006_2022-02-03.0001.mqd_R006_Run3_unstim.fcs · CD4_B2_d0_unstim_R014_2022-01-05.0001.mqd_R014_Run1_unstim.fcs · CD4_C1_d0_unstim_R007_2022-01-05.0001.mqd_R007_Run1_unstim.fcs · CD4_C1_d0_unstim_R018_2022-01-17.0001.mqd_R018_Run2_unstim.fcs · CD4_C1_d28_unstim_R008_2022-02-03.0001.mqd_R008_Run3_unstim.fcs · CD4_C1_d28_unstim_R018_2022-02-14.0001.mqd_R018_run4_unstim.fcs · CD4_C2_d0_unstim_R015_2022-01-05.0001.mqd_R015_Run1_unstim.fcs · CD4_D1_d0_unstim_R008_2022-01-05.0001.mqd_R008_Run1_unstim.fcs · CD4_D1_d0_unstim_R019_2022-01-17.0001.mqd_R019_Run2_unstim.fcs · CD4_D1_d28_unstim_R009_2022-02-03.0001.mqd_R009_Run3_unstim.fcs · CD4_D1_d28_unstim_R019_2022-02-14.0001.mqd_R019_run4_unstim.fcs · CD4_E1_d0_unstim_R009_2022-01-05.0001.mqd_R009_Run1_unstim.fcs · CD4_E1_d0_unstim_R020_2022-01-17.0001.mqd_R020_Run2_unstim.fcs · CD4_E1_d28_unstim_R010_2022-02-03.0001.mqd_R010_Run3_unstim.fcs · CD4_E1_d28_unstim_R020_2022-02-14.0001.mqd_R020_run4_unstim.fcs · CD4_F1_d0_unstim_R010_2022-01-05.0001.mqd_R010_Run1_unstim.fcs · CD4_F1_d0_unstim_R021_2022-01-17.0001.mqd_R021_Run2_unstim.fcs · CD4_F1_d28_unstim_R012_2022-02-03.0001.mqd_R012_Run3_unstim.fcs · CD4_F1_d28_unstim_R021_2022-02-14.0001.mqd_R021_run4_unstim.fcs · CD4_G1_d0_unstim_R011_2022-01-05.0001.mqd_R011_Run1_unstim.fcs · CD4_G1_d28_unstim_R013_2022-02-03.0001.mqd_R013_Run3_unstim.fcs · CD4_H1_d0_unstim_R012_2022-01-05.0001.mqd_R012_Run1_unstim.fcs · CD4_H1_d28_unstim_R015_2022-02-03.0001.mqd_R015_Run3_unstim.fcs
· SEB CD4_A3_d0_SEB_R020_2022-01-17.0001.mqd_R020_Run2_SEB.fcs · CD4_A3_d28_SEB_R005_2022-02-03.0001.mqd_R005_Run3_SEB.fcs · CD4_A3_d28_SEB_R020_2022-02-14.0001.mqd_R020_run4_SEB.fcs · CD4_A4_d0_SEB_R007_2022-01-05.0001.mqd_R007_Run1_SEB.fcs · CD4_A5_d0_SEB_R015_2022-01-05.0001.mqd_R015_Run1_SEB.fcs · CD4_B3_d0_SEB_R021_2022-01-17.0001.mqd_R021_Run2_SEB.fcs · CD4_B3_d28_SEB_R006_2022-02-03.0001.mqd_R006_Run3_SEB.fcs · CD4_B3_d28_SEB_R021_2022-02-14.0001.mqd_R021_run4_SEB.fcs · CD4_B4_d0_SEB_R008_2022-01-05.0001.mqd_R008_Run1_SEB.fcs · CD4_C3_d28_SEB_R008_2022-02-03.0001.mqd_R008_Run3_SEB.fcs · CD4_C4_d0_SEB_R009_2022-01-05.0001.mqd_R009_Run1_SEB.fcs · CD4_C4_d28_SEB_R011_2022-02-03.0001.mqd_R011_Run3_SEB.fcs · CD4_D3_d28_SEB_R009_2022-02-03.0001.mqd_R009_Run3_SEB.fcs · CD4_D4_d0_SEB_R010_2022-01-05.0001.mqd_R010_Run1_SEB.fcs · CD4_E2_d0_SEB_R016_2022-01-17.0001.mqd_R016_Run2_SEB.fcs · CD4_E2_d28_SEB_R016_2022-02-14.0001.mqd_R016_run4_SEB.fcs · CD4_E3_d28_SEB_R010_2022-02-03.0001.mqd_R010_Run3_SEB.fcs · CD4_E4_d0_SEB_R011_2022-01-05.0001.mqd_R011_Run1_SEB.fcs · CD4_F2_d0_SEB_R017_2022-01-17.0001.mqd_R017_Run2_SEB.fcs · CD4_F2_d27_SEB_R017_2022-02-14.0001.mqd_R017_run4_SEB.fcs · CD4_F3_d28_SEB_R012_2022-02-03.0001.mqd_R012_Run3_SEB.fcs · CD4_F4_d0_SEB_R012_2022-01-05.0001.mqd_R012_Run1_SEB.fcs · CD4_G2_d0_SEB_R018_2022-01-17.0001.mqd_R018_Run2_SEB.fcs · CD4_G2_d28_SEB_R018_2022-02-14.0001.mqd_R018_run4_SEB.fcs · CD4_G3_d0_SEB_R005_2022-01-05.0001.mqd_R005_Run1_SEB.fcs · CD4_G3_d28_SEB_R013_2022-02-03.0001.mqd_R013_Run3_SEB.fcs · CD4_G4_d0_SEB_R013_2022-01-05.0001.mqd_R013_Run1_SEB.fcs · CD4_H2_d0_SEB_R019_2022-01-17.0001.mqd_R019_Run2_SEB.fcs · CD4_H2_d28_SEB_R019_2022-02-14.0001.mqd_R019_run4_SEB.fcs · CD4_H3_d0_SEB_R006_2022-01-05.0001.mqd_R006_Run1_SEB.fcs · CD4_H3_d28_SEB_R015_2022-02-03.0001.mqd_R015_Run3_SEB.fcs · CD4_H4_d0_SEB_R014_2022-01-05.0001.mqd_R014_Run1_SEB.fcs
· Spike CD4_A2_d0_Spike_R018_2022-01-17.0001.mqd_R018_Run2_Spike.fcs · CD4_A2_d28_Spike_R005_2022-02-03.0001.mqd_R005_Run3_Spike.fcs · CD4_A2_d28_Spike_R018_2022-02-14.0001.mqd_R018_run4_Spike.fcs · CD4_A3_d0_Spike_R010_2022-01-05.0001.mqd_R010_Run1_Spike.fcs · CD4_B2_d0_Spike_R019_2022-01-17.0001.mqd_R019_Run2_Spike.fcs · CD4_B2_d28_Spike_R006_2022-02-03.0001.mqd_R006_Run3_Spike.fcs · CD4_B2_d28_Spike_R019_2022-02-14.0001.mqd_R019_run4_Spike.fcs · CD4_B3_d0_Spike_R011_2022-01-05.0001.mqd_R011_Run1_Spike.fcs · CD4_B4_d28_Spike_R011_2022-02-03.0001.mqd_R011_Run3_Spike.fcs · CD4_C2_d0_Spike_R020_2022-01-17.0001.mqd_R020_Run2_Spike.fcs · CD4_C2_d28_Spike_R008_2022-02-03.0001.mqd_R008_Run3_Spike.fcs · CD4_C2_d28_Spike_R020_2022-02-14.0001.mqd_R020_run4_Spike.fcs · CD4_C3_d0_Spike_R012_2022-01-05.0001.mqd_R012_Run1_Spike.fcs · CD4_D2_d0_Spike_R005_2022-01-05.0001.mqd_R005_Run1_Spike.fcs · CD4_D2_d0_Spike_R021_2022-01-17.0001.mqd_R021_Run2_Spike.fcs · CD4_D2_d28_Spike_R009_2022-02-03.0001.mqd_R009_Run3_Spike.fcs · CD4_D2_d28_Spike_R021_2022-02-14.0001.mqd_R021_run4_Spike.fcs · CD4_D3_d0_Spike_R013_2022-01-05.0001.mqd_R013_Run1_Spike.fcs · CD4_E2_d0_Spike_R006_2022-01-05.0001.mqd_R006_Run1_Spike.fcs · CD4_E2_d28_Spike_R010_2022-02-03.0001.mqd_R010_Run3_Spike.fcs · CD4_E3_d0_Spike_R014_2022-01-05.0001.mqd_R014_Run1_Spike.fcs · CD4_F2_d0_Spike_R007_2022-01-05.0001.mqd_R007_Run1_Spike.fcs · CD4_F2_d28_Spike_R012_2022-02-03.0001.mqd_R012_Run3_Spike.fcs · CD4_F3_d0_Spike_R015_2022-01-05.0001.mqd_R015_Run1_Spike.fcs · CD4_G1_d0_Spike_R016_2022-01-17.0001.mqd_R016_Run2_Spike.fcs · CD4_G1_d28_Spike_R016_2022-02-14.0001.mqd_R016_run4_Spike.fcs · CD4_G2_d0_Spike_R008_2022-01-05.0001.mqd_R008_Run1_Spike.fcs · CD4_G2_d28_Spike_R013_2022-02-03.0001.mqd_R013_Run3_Spike.fcs · CD4_H1_d0_Spike_R017_2022-01-17.0001.mqd_R017_Run2_Spike.fcs · CD4_H1_d27_Spike_R017_2022-02-14.0001.mqd_R017_run4_Spike.fcs · CD4_H2_d0_Spike_R009_2022-01-05.0001.mqd_R009_Run1_Spike.fcs · CD4_H2_d28_Spike_R015_2022-02-03.0001.mqd_R015_Run3_Spike.fcs

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