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Experiment Overview

Repository ID: FR-FCM-Z7DB Experiment name: Evaluating the function of NK cells that overexpress NKp30 or NKG2D or CD244 in the co-culture with autologous HIV-infected CD4+ T cells MIFlowCyt score: 97.00%
Primary researcher: Ruoxi Pi PI/manager: Catherine Blish Uploaded by: Ruoxi Pi
Experiment dates: 2024-01-22 - 2024-02-03 Dataset uploaded: Apr 2024 Last updated: Oct 2025
Keywords: [NKG2D] [charge-altering releasable transporters (CART)] [natural killer (NK) cells] [human immunodeficiency virus (HIV)] [NKp30] [CD244 (2B4)] Manuscripts:
Organizations: Stanford University, Department of Medicine, Division of Infectious Diseases, Palo Alto, California (USA)
Purpose: In our previous experiments, we demonstrated that NKp30, or NKG2D, or CD244 can be overexpressed in human primary NK cells through charge-altering releasable transporters (CART)/mRNA transfection and the transfected NK cells can be indicated with the co-expression of GFP. In this experiment, we are comparing the functional response of NK cells (indicated with degranulation marker CD107a, and cytokines, IFNgamma and TNFalpha) that overexpress NKp30, or NKG2D, or CD244 and the ones with natural levels of expression of these receptors, when co-cultured with autologous HIV-infected and mock-infected CD4+ T cells. This experiment will indicate the function of NKp30, NKG2D and CD244 in NK cell recognition of HIV.
Conclusion: In transfected NK cells (GFP+) where NKp30 or NKG2D were overexpressed, the percentage of functional NK cells (positive for any of CD107a, IFNgamma, and TNFalpha) was higher than in untransfected NK cells (GFP-) in the same well of co-culturing with HIV-infected or mock-infected CD4+ T cells. CD244 overexpression resulted in increased percentage of functional NK cells compared to NK cells that had natural level of CD244 expression. When NK cells were co-cultured with resting CD4+ T cells (the ones that were not activated to become susceptible to HIV infection), NKp30, or NKG2D, or CD244 overexpression didn't cause any noticeable change to the percentage of functional NK cells. These data suggest that NKp30 and NKG2D facilitate NK cell recognition of CD4+ T cells when their ligands are upregulated on CD4+ T cells during HIV infection. Increase of ligand expression caused by in vitro activation may also cause elevation of NK cell function. CD244 overexpression resulted in decreased NK cell function when interacting with their ligands on CD4+ T cells.
Comments: Overexpression of NKp30, or NKG2D, or CD244 in NK cells was achieved through charge-altering releasable transporters (CARTs) transfection to deliver the encoding mRNAs. NK cells were co-transfected with GFP mRNA to distinguish the NK cells that have translated the exogenous mRNA from the ones that naturally express these receptors. When transfected with NKG2D mRNA, NK cells were co-transfected with DAP10 mRNA, which is essential for the surface expression of the exogenous NKG2D. Three control groups were included, one with untransfected NK cells, another with NK cells transfected with GFP mRNA alone, and the other with NK cells co-transfected with DAP10+GFP mRNA. The mass ratio of co-transfected mRNA in each group is 1:1, 3:1:3, 1:1, and 1:6 for NKp30+GFP, NKG2D+DAP10+GFP, CD244+GFP, and DAP10+GFP, respectively. NK cell transfection was performed by adding CART/mRNA complex that contains 100 ng of total mRNA into each 500,000 NK cells in a non-tissue culture treated U-bottom 96-well plate. NK cells were cultured overnight after the transfection and co-cultured with autologous HIV infected CD4+ T cells or mock-infected CD4+ T cells at effector-to-target ratio 1:4 for 4-4.5 hours. Analysis of NK cells that were cultured alone indicates the background level of NK cell function. NK cell function elevation due to interaction with CD4+ T cells was evaluated by background subtraction calculated as the percentageo of functional NK cells in the presence of CD4+ T cells minus the percentage of functional NK cells in the absence of CD4+ T cells. The experiment was performed in 2 batches. The first batch contains the fcs files whose names start with 240126, with cells from 3 donors, donor RP47 (donor 3), donor RP48 (donor 4), donor RP50 (donor 6). The second batch contains the fcs files whose names start with 240203, with cells from 3 donors, donor RP44 (donor 2), donor RP49 (donor 5), and donor RP28 (donor 1).
Funding: This work was supported by the National Institutes of Health (NIH R01 AI161803).
Quality control: None


Experiment variables

Sample Type
· NK cells were co-cultured with autologous HIV-infected CD4+ T cells 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A1 NKp30_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A5 NKG2D_DAP10_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A9 2B4_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C1 GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C5 Untranfected+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C9 DAP10_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E1 NKp30_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E5 NKG2D_DAP10_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E9 2B4_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G1 GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G5 Untranfected+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G9 DAP10_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A1 NKp30_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A5 NKG2D_DAP10_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A9 2B4_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C1 GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C5 Untranfected+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C9 DAP10_GFP+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A1 NKp30_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A5 NKG2D_DAP10_GFP 3_1_3+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A9 2B4_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C1 GFP+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C5 Untransfected+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C9 DAP10_GFP 1_6+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E1 NKp30_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E5 NKG2D_DAP10_GFP 3_1_3+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E9 2B4_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G1 GFP+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G5 Untransfected+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G9 DAP10_GFP 1_6+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A1 NKp30_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A5 NKG2D_DAP10_GFP 3_1_3+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A9 2B4_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C1 GFP+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C5 Untransfected+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C9 DAP10_GFP 1_6+Inf_Unmixed.fcs
· NK cells were co-cultured with autologous mock-infected CD4+ T cells 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A10 2B4_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A2 NKP30_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A6 NKG2D_DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C10 DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C2 GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C6 Untransfected+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E10 2B4_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E2 NKP30_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E6 NKG2D_DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G10 DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G2 GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G6 Untransfected+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A10 2B4_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A2 NKP30_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A6 NKG2D_DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C10 DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C2 GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C6 Untransfected+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A10 2B4_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A2 NKp30_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A6 NKG2D_DAP10_GFP 3_1_3+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C10 DAP10_GFP 1_6+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C2 GFP+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C6 Untransfected+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E10 2B4_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E2 NKp30_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E6 NKG2D_DAP10_GFP 3_1_3+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G10 DAP10_GFP 1_6+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G2 GFP+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G6 Untransfected+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A10 2B4_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A2 NKp30_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A6 NKG2D_DAP10_GFP 3_1_3+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C10 DAP10_GFP 1_6+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C2 GFP+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C6 Untransfected+Uninf_Unmixed.fcs
· NK cells were co-cultured with autologous resting CD4+ T cells (isolated from PBMCs but not activated) 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A11 2B4_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A3 NKp30_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A7 NKG2D-DAP10_GFP 3_1_3+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C11 DAP10_GFP 1_6+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C3 GFP+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C7 Untransfected+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E11 2B4_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E3 NKp30_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E7 NKG2D-DAP10_GFP 3_1_3+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G3 GFP+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G7 Untransfected+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A11 2B4_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A3 NKp30_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A7 NKG2D-DAP10_GFP 3_1_3+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C11 DAP10_GFP 1_6+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C3 GFP+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C7 Untransfected+resting_Unmixed.fcs
· NK cells were cultured alone 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A11 2B4_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A3 NKp30_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A7 NKG2D_DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C11 DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C3 GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C7 Untransfected alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E11 2B4_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E3 NKp30_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E7 NKG2D_DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G11 DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G3 GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G7 Untransfected alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A11 2B4_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A3 NKp30_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A7 NKG2D_DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C11 DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C3 GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C7 Untransfected alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A12 2B4_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A4 NKp30_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A8 NKG2D_DAP10_GFP 3_1_3 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C12 DAP10_GFP 1_6 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C4 GFP NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C8 Untransfected NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E12 2B4_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E4 NKp30_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E8 NKG2D_DAP10_GFP 3_1_3 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G12 DAP10_GFP 1_6 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G4 GFP NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G8 Untransfected NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A12 2B4_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A4 NKp30_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A8 NKG2D_DAP10_GFP 3_1_3 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C12 DAP10_GFP 1_6 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C4 GFP NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C8 Untransfected NK alone_Unmixed.fcs

Conditions
· NK cells were transfected with CART ONA/mRNA complex that contains NKp30 mRNA and GFP mRNA 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A1 NKp30_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A2 NKP30_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A3 NKp30_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E1 NKp30_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E2 NKP30_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E3 NKp30_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A1 NKp30_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A2 NKP30_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A3 NKp30_GFP alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A1 NKp30_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A2 NKp30_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A3 NKp30_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A4 NKp30_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E1 NKp30_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E2 NKp30_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E3 NKp30_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E4 NKp30_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A1 NKp30_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A2 NKp30_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A3 NKp30_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A4 NKp30_GFP 1_1 NK alone_Unmixed.fcs
· NK cells were transfected with CART ONA/mRNA complex that contains NKG2D mRNA, DAP10 mRNA and GFP mRNA 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A5 NKG2D_DAP10_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A6 NKG2D_DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A7 NKG2D_DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E5 NKG2D_DAP10_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E6 NKG2D_DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E7 NKG2D_DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A5 NKG2D_DAP10_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A6 NKG2D_DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A7 NKG2D_DAP10_GFP alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A5 NKG2D_DAP10_GFP 3_1_3+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A6 NKG2D_DAP10_GFP 3_1_3+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A7 NKG2D-DAP10_GFP 3_1_3+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A8 NKG2D_DAP10_GFP 3_1_3 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E5 NKG2D_DAP10_GFP 3_1_3+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E6 NKG2D_DAP10_GFP 3_1_3+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E7 NKG2D-DAP10_GFP 3_1_3+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E8 NKG2D_DAP10_GFP 3_1_3 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A5 NKG2D_DAP10_GFP 3_1_3+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A6 NKG2D_DAP10_GFP 3_1_3+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A7 NKG2D-DAP10_GFP 3_1_3+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A8 NKG2D_DAP10_GFP 3_1_3 NK alone_Unmixed.fcs
· NK cells were transfected with CART ONA/mRNA complex that contains CD244 mRNA and GFP mRNA 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A10 2B4_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A11 2B4_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A9 2B4_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E10 2B4_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E11 2B4_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E9 2B4_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A10 2B4_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A11 2B4_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A9 2B4_GFP+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A10 2B4_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A11 2B4_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A12 2B4_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A9 2B4_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E10 2B4_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E11 2B4_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E12 2B4_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E9 2B4_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A10 2B4_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A11 2B4_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A12 2B4_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A9 2B4_GFP 1_1+Inf_Unmixed.fcs
· NK cells were transfected with CART ONA/mRNA complex that contains DAP10 mRNA and GFP mRNA 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C10 DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C11 DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C9 DAP10_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G10 DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G11 DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G9 DAP10_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C10 DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C11 DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C9 DAP10_GFP+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C10 DAP10_GFP 1_6+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C11 DAP10_GFP 1_6+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C12 DAP10_GFP 1_6 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C9 DAP10_GFP 1_6+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G10 DAP10_GFP 1_6+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G12 DAP10_GFP 1_6 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G9 DAP10_GFP 1_6+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C10 DAP10_GFP 1_6+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C11 DAP10_GFP 1_6+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C12 DAP10_GFP 1_6 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C9 DAP10_GFP 1_6+Inf_Unmixed.fcs
· NK cells were transfected with CART ONA/mRNA complex that contains GFP mRNA 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C1 GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C2 GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C3 GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G1 GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G2 GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G3 GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C1 GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C2 GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C3 GFP alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C1 GFP+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C2 GFP+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C3 GFP+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C4 GFP NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G1 GFP+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G2 GFP+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G3 GFP+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G4 GFP NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C1 GFP+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C2 GFP+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C3 GFP+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C4 GFP NK alone_Unmixed.fcs
· NK cells were treated with GFP mRNA that was not complexed with CART ONA (untransfected) 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C5 Untranfected+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C6 Untransfected+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C7 Untransfected alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G5 Untranfected+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G6 Untransfected+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G7 Untransfected alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C5 Untranfected+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C6 Untransfected+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C7 Untransfected alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C5 Untransfected+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C6 Untransfected+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C7 Untransfected+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C8 Untransfected NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G5 Untransfected+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G6 Untransfected+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G7 Untransfected+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G8 Untransfected NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C5 Untransfected+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C6 Untransfected+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C7 Untransfected+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C8 Untransfected NK alone_Unmixed.fcs

Individuals
· Donor RP28 (donor 1) 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A1 NKp30_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A10 2B4_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A11 2B4_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A12 2B4_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A2 NKp30_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A3 NKp30_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A4 NKp30_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A5 NKG2D_DAP10_GFP 3_1_3+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A6 NKG2D_DAP10_GFP 3_1_3+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A7 NKG2D-DAP10_GFP 3_1_3+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A8 NKG2D_DAP10_GFP 3_1_3 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-A9 2B4_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C1 GFP+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C10 DAP10_GFP 1_6+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C11 DAP10_GFP 1_6+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C12 DAP10_GFP 1_6 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C2 GFP+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C3 GFP+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C4 GFP NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C5 Untransfected+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C6 Untransfected+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C7 Untransfected+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C8 Untransfected NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 2-RP28-C9 DAP10_GFP 1_6+Inf_Unmixed.fcs
· Donor RP44 (donor 2) 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A1 NKp30_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A10 2B4_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A11 2B4_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A12 2B4_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A2 NKp30_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A3 NKp30_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A4 NKp30_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A5 NKG2D_DAP10_GFP 3_1_3+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A6 NKG2D_DAP10_GFP 3_1_3+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A7 NKG2D-DAP10_GFP 3_1_3+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A8 NKG2D_DAP10_GFP 3_1_3 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-A9 2B4_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C1 GFP+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C10 DAP10_GFP 1_6+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C11 DAP10_GFP 1_6+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C12 DAP10_GFP 1_6 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C2 GFP+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C3 GFP+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C4 GFP NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C5 Untransfected+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C6 Untransfected+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C7 Untransfected+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C8 Untransfected NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP44-C9 DAP10_GFP 1_6+Inf_Unmixed.fcs
· Donor RP47 (donor 3) 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A1 NKp30_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A10 2B4_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A11 2B4_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A2 NKP30_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A3 NKp30_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A5 NKG2D_DAP10_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A6 NKG2D_DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A7 NKG2D_DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-A9 2B4_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C1 GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C10 DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C11 DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C2 GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C3 GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C5 Untranfected+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C6 Untransfected+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C7 Untransfected alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP47-C9 DAP10_GFP+Inf_Unmixed.fcs
· Donor RP48 (donor 4) 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E1 NKp30_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E10 2B4_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E11 2B4_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E2 NKP30_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E3 NKp30_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E5 NKG2D_DAP10_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E6 NKG2D_DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E7 NKG2D_DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-E9 2B4_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G1 GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G10 DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G11 DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G2 GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G3 GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G5 Untranfected+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G6 Untransfected+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G7 Untransfected alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 1-RP48-G9 DAP10_GFP+Inf_Unmixed.fcs
· Donor RP49 (donor 5) 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E1 NKp30_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E10 2B4_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E11 2B4_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E12 2B4_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E2 NKp30_GFP 1_1+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E3 NKp30_GFP 1_1+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E4 NKp30_GFP 1_1 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E5 NKG2D_DAP10_GFP 3_1_3+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E6 NKG2D_DAP10_GFP 3_1_3+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E7 NKG2D-DAP10_GFP 3_1_3+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E8 NKG2D_DAP10_GFP 3_1_3 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-E9 2B4_GFP 1_1+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G1 GFP+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G10 DAP10_GFP 1_6+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G12 DAP10_GFP 1_6 NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G2 GFP+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G3 GFP+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G4 GFP NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G5 Untransfected+Inf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G6 Untransfected+Uninf_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G7 Untransfected+resting_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G8 Untransfected NK alone_Unmixed.fcs · 240203 NK cells CD4 T cells co-culture 2nd batch-Plate 1-RP49-G9 DAP10_GFP 1_6+Inf_Unmixed.fcs
· Donor RP50 (donor 6) 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A1 NKp30_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A10 2B4_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A11 2B4_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A2 NKP30_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A3 NKp30_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A5 NKG2D_DAP10_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A6 NKG2D_DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A7 NKG2D_DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-A9 2B4_GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C1 GFP+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C10 DAP10_GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C11 DAP10_GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C2 GFP+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C3 GFP alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C5 Untranfected+Inf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C6 Untransfected+Uninf_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C7 Untransfected alone_Unmixed.fcs · 240126 Co-culture of NK cells and CD4 T cells 1st-Plate 2-RP50-C9 DAP10_GFP+Inf_Unmixed.fcs

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