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Experiment Overview

Repository ID: FR-FCM-Z7H5 Experiment name: Evaluating the role of individual NK cell ligands in HIV-specific recognition by NK cells MIFlowCyt score: 97.00%
Primary researcher: Ruoxi Pi PI/manager: Catherine Blish Uploaded by: Ruoxi Pi
Experiment dates: 2020-03-16 - 2020-07-31 Dataset uploaded: May 2024 Last updated: Oct 2025
Keywords: [CRISPR] [natural killer (NK) cells] [human immunodeficiency virus (HIV)] Manuscripts:
Organizations: Stanford University, Department of Medicine, Division of Infectious Diseases, Palo Alto, California (USA)
Purpose: In our previous experiments, we performed phenotypic analysis on NK cells that had been co-cultured with HIV-infected or mock-infected CD4+ T cells to identify the NK cell receptors that are predictive of NK cells that are functionally responding to HIV-infected CD4+ T cells. We also analyzed CD4+ T cells for the activating NK cell ligands that are upregulated during HIV infection. We identified 8 candidate receptor-ligand pairs that can potentially contribute to the recognition of HIV by NK cells. In this experiment, we applied CRISPR editing on primary CD4+ T cells to knockout individual genes that encode the candidate ligands, infected the edited CD4+ T cells with HIV, co-cultured autologous NK cells with these CD4+ T cells at effector-to-target ratio 1:1 and evaluated the functional response of NK cells. Reduction in NK cell function compared to the unedited control group would indicate a facilitating role of the knocked-out gene in NK cell recognition during HIV infection.
Conclusion: In this experiment, we identified 6 individual ligands, including B7-H6, LFA-3, MICA, MICB, ULBP1, and ULBP2 to facilitate the HIV-specific response in autologous NK cells.
Comments: The functional response of NK cells was evaluated through the staining of functional markers, including CD107a, IFNgamma, and TNFalpha, and functional NK cells are defined as the ones that are stained positive for any of the three markers. The percentage of functional NK cells were analyzed in the groups where NK cells were co-cultured with HIV-infected or mock-infected CD4+ T cells. The level of HIV-specific response was evaluated by subtracting the percentage of functional NK cells when co-cultured with mock-infected CD4+ T cells from the percentage of functional NK cells when co-cultured with HIV-infected CD4+ T cells. HIV-specific response to edited CD4+ T cells was compared to unedited CD4+ T cells, which indicated the role of each NK cell ligand in HIV recognition. In this experiment, we used HIV strain Q23-17 to infect CD4+ T cells (Poss & Overbaugh, 1999). For donor 1 and 2, expression of each ligand was analyzed in the unedited CD4+ T cells and CD4+ T cells that were knocked out of the respective gene.
Funding: This work was supported by the National Institutes of Health (NIH DP1 DA046089, C.A.B).
Quality control: None


Experiment variables

Conditions
· CD4+ T cells with AAVS1 edited donor 1 HIV AAVS1.fcs · donor 1 mock AAVS1.fcs · donor 2 HIV AAVS1.fcs · donor 2 mock AAVS1.fcs · 87 HIV AAVS1.fcs · 87 mock AAVS1.fcs · 88 HIV AAVS1.fcs · 88 mock AAVS1.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-G7 AAVS1 edited_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-G8 AAVS1 edited_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-G10 AAVS1 edited_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-G11 AAVS1 edited_Unmixed.fcs
· CD4+ T cells were knocked out of B7-H6 donor 1 HIV B7-H6 pool.fcs · donor 1 mock B7-H6 pool.fcs · donor 2 HIV B7-H6 pool.fcs · donor 2 mock B7-H6 pool.fcs · 87 HIV B7-H6 pool.fcs · 87 mock B7-H6 pool.fcs · 88 HIV B7-H6 pool.fcs · 88 mock B7-H6 pool.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A1 B7-H6 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B1 B7-H6 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D1 B7-H6 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E1 B7-H6 knockout_Unmixed.fcs
· CD4+ T cells were knocked out of CD48 donor 1 HIV CD48 pool.fcs · donor 1 mock CD48 pool.fcs · donor 2 HIV CD48 pool.fcs · donor 2 mock CD48 pool.fcs · 87 HIV CD48 pool.fcs · 87 mock CD48 pool.fcs · 88 HIV CD48 pool.fcs · 88 mock CD48 pool.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A2 CD48 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B2 CD48 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D2 CD48 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E2 CD48 knockout_Unmixed.fcs
· CD4+ T cells were knocked out of Nectin-2 donor 1 HIV Nectin-2 pool.fcs · donor 1 mock Nectin-2 pool.fcs · donor 2 HIV Nectin-2 pool.fcs · donor 2 mock Nectin-2 pool.fcs · 87 HIV Nectin-2 pool.fcs · 87 mock Nectin-2 pool.fcs · 88 HIV Nectin-2 pool.fcs · 88 mock Nectin-2 pool.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A3 Nectin-2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B3 Nectin-2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D3 Nectin-2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E3 Nectin-2 knockout_Unmixed.fcs
· CD4+ T cells were knocked out of MICA donor 1 HIV MIC-A pool.fcs · donor 1 mock MIC-A pool.fcs · donor 2 HIV MIC-A pool.fcs · donor 2 mock MIC-A pool.fcs · 87 HIV MICA pool.fcs · 87 mock MICA pool.fcs · 88 HIV MICA pool.fcs · 88 mock MICA pool.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A5 MICA knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B5 MICA knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D5 MICA knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E5 MICA knockout_Unmixed.fcs
· CD4+ T cells were knocked out of MICB donor 1 HIV MIC-B pool.fcs · donor 1 mock MIC-B pool.fcs · donor 2 HIV MIC-B pool.fcs · donor 2 mock MIC-B pool.fcs · 87 HIV MICB pool.fcs · 87 mock MICB pool.fcs · 88 HIV MICB pool.fcs · 88 mock MICB pool.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A6 MICB knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B6 MICB knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D6 MICB knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E6 MICB knockout_Unmixed.fcs
· CD4+ T cells were knocked out of ULBP1 donor 1 HIV ULBP1 pool.fcs · donor 1 mock ULBP1 pool.fcs · donor 2 HIV ULBP1 pool.fcs · donor 2 mock ULBP1 pool.fcs · 87 HIV ULBP1 pool.fcs · 87 mock ULBP1 pool.fcs · 88 HIV ULBP1 pool.fcs · 88 mock ULBP1 pool.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A7 ULBP1 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B7 ULBP1 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D7 ULBP1 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E7 ULBP1 knockout_Unmixed.fcs
· CD4+ T cells were knocked out of ULBP2 donor 1 HIV ULBP2 pool.fcs · donor 1 mock ULBP2 pool.fcs · donor 2 HIV ULBP2 pool.fcs · donor 2 mock ULBP2 pool.fcs · 87 HIV ULBP2 pool.fcs · 87 mock ULBP2 pool.fcs · 88 HIV ULBP2 pool.fcs · 88 mock ULBP2 pool.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A8 ULBP2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B8 ULBP2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D8 ULBP2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E8 ULBP2 knockout_Unmixed.fcs
· CD4+ T cells were not edited donor 1 HIV NT B7-H6.fcs · donor 1 HIV NT CD48.fcs · donor 1 HIV NT CD58.fcs · donor 1 HIV NT MIC-A.fcs · donor 1 HIV NT MIC-B.fcs · donor 1 HIV NT Nectin-2.fcs · donor 1 HIV NT ULBP1.fcs · donor 1 HIV NT ULBP2.fcs · donor 1 mock NT B7-H6.fcs · donor 1 mock NT CD48.fcs · donor 1 mock NT CD58.fcs · donor 1 mock NT MIC-A.fcs · donor 1 mock NT MIC-B.fcs · donor 1 mock NT Nectin-2.fcs · donor 1 mock NT ULBP1.fcs · donor 1 mock NT ULBP2.fcs · donor 2 HIV NT B7-H6.fcs · donor 2 HIV NT CD48.fcs · donor 2 HIV NT CD58.fcs · donor 2 HIV NT MIC-A.fcs · donor 2 HIV NT MIC-B.fcs · donor 2 HIV NT Nectin-2.fcs · donor 2 HIV NT ULBP1.fcs · donor 2 HIV NT ULBP2.fcs · donor 2 mock NT B7-H6.fcs · donor 2 mock NT CD48.fcs · donor 2 mock NT CD58.fcs · donor 2 mock NT MIC-A.fcs · donor 2 mock NT MIC-B.fcs · donor 2 mock NT Nectin-2.fcs · donor 2 mock NT ULBP1.fcs · donor 2 mock NT ULBP2.fcs · 87 HIV NT.fcs · 87 mock NT.fcs · 88 HIV NT.fcs · 88 mock NT.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-H7 NT_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-H8 NT_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-H10 NT_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-H11 NT_Unmixed.fcs
· CD4+ T cells were knocked out of LFA-3 donor 1 HIV CD58 pool.fcs · donor 1 mock CD58 pool.fcs · donor 2 HIV CD58 pool.fcs · donor 2 mock CD58 pool.fcs · 87 HIV CD58 pool.fcs · 87 mock CD58 pool.fcs · 88 HIV CD58 pool.fcs · 88 mock CD58 pool.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A4 LFA-3 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B4 LFA-3 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D4 LFA-3 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E4 LFA-3 knockout_Unmixed.fcs

Individuals
· Donor 1 donor 1 HIV AAVS1.fcs · donor 1 HIV B7-H6 pool.fcs · donor 1 HIV CD48 pool.fcs · donor 1 HIV CD58 pool.fcs · donor 1 HIV MIC-A pool.fcs · donor 1 HIV MIC-B pool.fcs · donor 1 HIV NT B7-H6.fcs · donor 1 HIV NT CD48.fcs · donor 1 HIV NT CD58.fcs · donor 1 HIV NT MIC-A.fcs · donor 1 HIV NT MIC-B.fcs · donor 1 HIV NT Nectin-2.fcs · donor 1 HIV NT ULBP1.fcs · donor 1 HIV NT ULBP2.fcs · donor 1 HIV Nectin-2 pool.fcs · donor 1 HIV ULBP1 pool.fcs · donor 1 HIV ULBP2 pool.fcs · donor 1 NK alone 2.fcs · donor 1 NK alone.fcs · donor 1 mock AAVS1.fcs · donor 1 mock B7-H6 pool.fcs · donor 1 mock CD48 pool.fcs · donor 1 mock CD58 pool.fcs · donor 1 mock MIC-A pool.fcs · donor 1 mock MIC-B pool.fcs · donor 1 mock NT B7-H6.fcs · donor 1 mock NT CD48.fcs · donor 1 mock NT CD58.fcs · donor 1 mock NT MIC-A.fcs · donor 1 mock NT MIC-B.fcs · donor 1 mock NT Nectin-2.fcs · donor 1 mock NT ULBP1.fcs · donor 1 mock NT ULBP2.fcs · donor 1 mock Nectin-2 pool.fcs · donor 1 mock ULBP1 pool.fcs · donor 1 mock ULBP2 pool.fcs
· Donor 2 donor 2 HIV AAVS1.fcs · donor 2 HIV B7-H6 pool.fcs · donor 2 HIV CD48 pool.fcs · donor 2 HIV CD58 pool.fcs · donor 2 HIV MIC-A pool.fcs · donor 2 HIV MIC-B pool.fcs · donor 2 HIV NT B7-H6.fcs · donor 2 HIV NT CD48.fcs · donor 2 HIV NT CD58.fcs · donor 2 HIV NT MIC-A.fcs · donor 2 HIV NT MIC-B.fcs · donor 2 HIV NT Nectin-2.fcs · donor 2 HIV NT ULBP1.fcs · donor 2 HIV NT ULBP2.fcs · donor 2 HIV Nectin-2 pool.fcs · donor 2 HIV ULBP1 pool.fcs · donor 2 HIV ULBP2 pool.fcs · donor 2 NK alone 2.fcs · donor 2 NK alone.fcs · donor 2 mock AAVS1.fcs · donor 2 mock B7-H6 pool.fcs · donor 2 mock CD48 pool.fcs · donor 2 mock CD58 pool.fcs · donor 2 mock MIC-A pool.fcs · donor 2 mock MIC-B pool.fcs · donor 2 mock NT B7-H6.fcs · donor 2 mock NT CD48.fcs · donor 2 mock NT CD58.fcs · donor 2 mock NT MIC-A.fcs · donor 2 mock NT MIC-B.fcs · donor 2 mock NT Nectin-2.fcs · donor 2 mock NT ULBP1.fcs · donor 2 mock NT ULBP2.fcs · donor 2 mock Nectin-2 pool.fcs · donor 2 mock ULBP1 pool.fcs · donor 2 mock ULBP2 pool.fcs
· Donor 87 87 HIV AAVS1.fcs · 87 HIV B7-H6 pool.fcs · 87 HIV CD48 pool.fcs · 87 HIV CD58 pool.fcs · 87 HIV MICA pool.fcs · 87 HIV MICB pool.fcs · 87 HIV NT.fcs · 87 HIV Nectin-2 pool.fcs · 87 HIV ULBP1 pool.fcs · 87 HIV ULBP2 pool.fcs · 87 NK alone 1.fcs · 87 NK alone 2.fcs · 87 mock AAVS1.fcs · 87 mock B7-H6 pool.fcs · 87 mock CD48 pool.fcs · 87 mock CD58 pool.fcs · 87 mock MICA pool.fcs · 87 mock MICB pool.fcs · 87 mock NT.fcs · 87 mock Nectin-2 pool.fcs · 87 mock ULBP1 pool.fcs · 87 mock ULBP2 pool.fcs
· Donor 88 88 HIV AAVS1.fcs · 88 HIV B7-H6 pool.fcs · 88 HIV CD48 pool.fcs · 88 HIV CD58 pool.fcs · 88 HIV MICA pool.fcs · 88 HIV MICB pool.fcs · 88 HIV NT.fcs · 88 HIV Nectin-2 pool.fcs · 88 HIV ULBP1 pool.fcs · 88 HIV ULBP2 pool.fcs · 88 NK alone 1.fcs · 88 NK alone 2.fcs · 88 mock AAVS1.fcs · 88 mock B7-H6 pool.fcs · 88 mock CD48 pool.fcs · 88 mock CD58 pool.fcs · 88 mock MICA pool.fcs · 88 mock MICB pool.fcs · 88 mock NT.fcs · 88 mock Nectin-2 pool.fcs · 88 mock ULBP1 pool.fcs · 88 mock ULBP2 pool.fcs
· Donor 5 (RP53) 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK alone-G3 NK alone 1_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK alone-G9 NK alone 3_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK alone-H3 NK alone 2_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK alone-H9 NK alone 4_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A1 B7-H6 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A2 CD48 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A3 Nectin-2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A4 LFA-3 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A5 MICA knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A6 MICB knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A7 ULBP1 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A8 ULBP2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-G7 AAVS1 edited_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-H7 NT_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B1 B7-H6 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B2 CD48 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B3 Nectin-2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B4 LFA-3 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B5 MICA knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B6 MICB knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B7 ULBP1 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B8 ULBP2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-G8 AAVS1 edited_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-H8 NT_Unmixed.fcs
· Donor 6 (RP58) 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK alone-G12 NK alone 3_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK alone-G6 NK alone 1_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK alone-H12 NK alone 4_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK alone-H6 NK alone 2_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D1 B7-H6 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D2 CD48 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D3 Nectin-2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D4 LFA-3 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D5 MICA knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D6 MICB knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D7 ULBP1 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D8 ULBP2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-G10 AAVS1 edited_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-H10 NT_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E1 B7-H6 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E2 CD48 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E3 Nectin-2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E4 LFA-3 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E5 MICA knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E6 MICB knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E7 ULBP1 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E8 ULBP2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-G11 AAVS1 edited_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-H11 NT_Unmixed.fcs

Sample Type
· NK cells were cocultured with HIV-infected CD4+ T cells donor 1 HIV AAVS1.fcs · donor 1 HIV B7-H6 pool.fcs · donor 1 HIV CD48 pool.fcs · donor 1 HIV CD58 pool.fcs · donor 1 HIV MIC-A pool.fcs · donor 1 HIV MIC-B pool.fcs · donor 1 HIV NT B7-H6.fcs · donor 1 HIV NT CD48.fcs · donor 1 HIV NT CD58.fcs · donor 1 HIV NT MIC-A.fcs · donor 1 HIV NT MIC-B.fcs · donor 1 HIV NT Nectin-2.fcs · donor 1 HIV NT ULBP1.fcs · donor 1 HIV NT ULBP2.fcs · donor 1 HIV Nectin-2 pool.fcs · donor 1 HIV ULBP1 pool.fcs · donor 1 HIV ULBP2 pool.fcs · donor 2 HIV AAVS1.fcs · donor 2 HIV B7-H6 pool.fcs · donor 2 HIV CD48 pool.fcs · donor 2 HIV CD58 pool.fcs · donor 2 HIV MIC-A pool.fcs · donor 2 HIV MIC-B pool.fcs · donor 2 HIV NT B7-H6.fcs · donor 2 HIV NT CD48.fcs · donor 2 HIV NT CD58.fcs · donor 2 HIV NT MIC-A.fcs · donor 2 HIV NT MIC-B.fcs · donor 2 HIV NT Nectin-2.fcs · donor 2 HIV NT ULBP1.fcs · donor 2 HIV NT ULBP2.fcs · donor 2 HIV Nectin-2 pool.fcs · donor 2 HIV ULBP1 pool.fcs · donor 2 HIV ULBP2 pool.fcs · 87 HIV AAVS1.fcs · 87 HIV B7-H6 pool.fcs · 87 HIV CD48 pool.fcs · 87 HIV CD58 pool.fcs · 87 HIV MICA pool.fcs · 87 HIV MICB pool.fcs · 87 HIV NT.fcs · 87 HIV Nectin-2 pool.fcs · 87 HIV ULBP1 pool.fcs · 87 HIV ULBP2 pool.fcs · 88 HIV AAVS1.fcs · 88 HIV B7-H6 pool.fcs · 88 HIV CD48 pool.fcs · 88 HIV CD58 pool.fcs · 88 HIV MICA pool.fcs · 88 HIV MICB pool.fcs · 88 HIV NT.fcs · 88 HIV Nectin-2 pool.fcs · 88 HIV ULBP1 pool.fcs · 88 HIV ULBP2 pool.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A1 B7-H6 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A2 CD48 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A3 Nectin-2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A4 LFA-3 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A5 MICA knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A6 MICB knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A7 ULBP1 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-A8 ULBP2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-G7 AAVS1 edited_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+infected T cells-H7 NT_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D1 B7-H6 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D2 CD48 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D3 Nectin-2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D4 LFA-3 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D5 MICA knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D6 MICB knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D7 ULBP1 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-D8 ULBP2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-G10 AAVS1 edited_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+infected T cells-H10 NT_Unmixed.fcs
· NK cells were cocultured with mock-infected CD4+ T cells donor 1 mock AAVS1.fcs · donor 1 mock B7-H6 pool.fcs · donor 1 mock CD48 pool.fcs · donor 1 mock CD58 pool.fcs · donor 1 mock MIC-A pool.fcs · donor 1 mock MIC-B pool.fcs · donor 1 mock NT B7-H6.fcs · donor 1 mock NT CD48.fcs · donor 1 mock NT CD58.fcs · donor 1 mock NT MIC-A.fcs · donor 1 mock NT MIC-B.fcs · donor 1 mock NT Nectin-2.fcs · donor 1 mock NT ULBP1.fcs · donor 1 mock NT ULBP2.fcs · donor 1 mock Nectin-2 pool.fcs · donor 1 mock ULBP1 pool.fcs · donor 1 mock ULBP2 pool.fcs · donor 2 mock AAVS1.fcs · donor 2 mock B7-H6 pool.fcs · donor 2 mock CD48 pool.fcs · donor 2 mock CD58 pool.fcs · donor 2 mock MIC-A pool.fcs · donor 2 mock MIC-B pool.fcs · donor 2 mock NT B7-H6.fcs · donor 2 mock NT CD48.fcs · donor 2 mock NT CD58.fcs · donor 2 mock NT MIC-A.fcs · donor 2 mock NT MIC-B.fcs · donor 2 mock NT Nectin-2.fcs · donor 2 mock NT ULBP1.fcs · donor 2 mock NT ULBP2.fcs · donor 2 mock Nectin-2 pool.fcs · donor 2 mock ULBP1 pool.fcs · donor 2 mock ULBP2 pool.fcs · 87 mock AAVS1.fcs · 87 mock B7-H6 pool.fcs · 87 mock CD48 pool.fcs · 87 mock CD58 pool.fcs · 87 mock MICA pool.fcs · 87 mock MICB pool.fcs · 87 mock NT.fcs · 87 mock Nectin-2 pool.fcs · 87 mock ULBP1 pool.fcs · 87 mock ULBP2 pool.fcs · 88 mock AAVS1.fcs · 88 mock B7-H6 pool.fcs · 88 mock CD48 pool.fcs · 88 mock CD58 pool.fcs · 88 mock MICA pool.fcs · 88 mock MICB pool.fcs · 88 mock NT.fcs · 88 mock Nectin-2 pool.fcs · 88 mock ULBP1 pool.fcs · 88 mock ULBP2 pool.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B1 B7-H6 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B2 CD48 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B3 Nectin-2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B4 LFA-3 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B5 MICA knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B6 MICB knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B7 ULBP1 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-B8 ULBP2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-G8 AAVS1 edited_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK+mock-infected T cells-H8 NT_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E1 B7-H6 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E2 CD48 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E3 Nectin-2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E4 LFA-3 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E5 MICA knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E6 MICB knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E7 ULBP1 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-E8 ULBP2 knockout_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-G11 AAVS1 edited_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK+mock-infected T cells-H11 NT_Unmixed.fcs
· NK cells were cultured alone donor 1 NK alone 2.fcs · donor 1 NK alone.fcs · donor 2 NK alone 2.fcs · donor 2 NK alone.fcs · 87 NK alone 1.fcs · 87 NK alone 2.fcs · 88 NK alone 1.fcs · 88 NK alone 2.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK alone-G3 NK alone 1_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK alone-G9 NK alone 3_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK alone-H3 NK alone 2_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP53 NK alone-H9 NK alone 4_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK alone-G12 NK alone 3_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK alone-G6 NK alone 1_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK alone-H12 NK alone 4_Unmixed.fcs · 250307 CRISPR T cells coculture RP53 RP58-Co-culture samples-RP58 NK alone-H6 NK alone 2_Unmixed.fcs

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