Experiment Overview
| Repository ID: | FR-FCM-Z7H8 | Experiment name: | Co-culture of NK cells and autologous HIV-infected CD4+ T cells in vitro | MIFlowCyt score: | 83.00% |
| Primary researcher: | Ruoxi Pi | PI/manager: | Catherine Blish | Uploaded by: | Ruoxi Pi |
| Experiment dates: | 2017-04-17 - 2017-05-05 | Dataset uploaded: | May 2024 | Last updated: | Oct 2025 |
| Keywords: | [CyTOF] [natural killer (NK) cells] [human immunodeficiency virus (HIV)] | Manuscripts: | |||
| Organizations: |
Stanford University, Department of Medicine, Division of Infectious Diseases, Palo Alto, California (USA)
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| Purpose: | In this experiment, we co-cultured NK cells with autologous HIV-infected CD4+ T cells in vitro at different effector-to-target (E:T) ratios. The purpose of this experiment was to optimize the co-culture system to evaluate NK cell response to autologous HIV-infected CD4+ T cells. | ||||
| Conclusion: | We observed consistent NK cell responses with cytolytic degranulation (by the expression of the degranulation marker CD107a), cytokine production (for the canonical NK cell cytokine IFN-ɣ), and viral suppression mediated by NK cells with a reduction in levels of p24+ cells. Both degranulation and cytokine production scaled negatively with increasing effector:target (E:T) ratio. Viral suppression scaled positively with E:T ratio. | ||||
| Comments: | This in vitro system allows us to probe specific interactions involved in NK cell recognition of HIV and the initiation of downstream HIV-specific responses. | ||||
| Funding: | This work was supported by the National Institutes of Health (NIH DP1 DA046089 and NIH R01 AI161803, C.A.B). | ||||
| Quality control: | None | ||||
Experiment variables
| Conditions | |
|---|---|
| · NK cells were co-cultured with autologous HIV-infected CD4+ T cells at effector-to-target ratio 4:1 | NZ2017-04-21C10Q23 + NK 4_1NZ01.0001.fcs · NZ2017-04-21D10Q23 + NK 4_1NZ02.0001.fcs · NZ2017-04-21E10Q23 + NK 4_1NZ03.0001.fcs · NZ2017-04-21F10Q23 + NK 4_1NZ04.0001.fcs · NZ2017-04-21G10Q23 + NK 4_1NZ05.0001.fcs · NZ2017-04-21H10Q23 + NK 4_1NZ06.0001.fcs · NZ2017-05-01C10Q23 + NK 4_1NZ07.0001.fcs · NZ2017-05-01D10Q23 + NK 4_1NZ08.0001.fcs · NZ2017-05-01E10Q23 + NK 4_1NZ09.0001.fcs · NZ2017-05-01F10Q23 + NK 4_1NZ11.0001.fcs · NZ2017-05-01G10Q23 + NK 4_1NZ13.0001.fcs · NZ2017-05-01H10Q23 + NK 4_1NZ14.0001.fcs |
| · NK cells were co-cultured with autologous HIV-infected CD4+ T cells at effector-to-target ratio 1:1 | NZ2017-04-21C9Q23 + NK 1_1NZ01.0001.fcs · NZ2017-04-21D9Q23 + NK 1_1NZ02.0001.fcs · NZ2017-04-21E9Q23 + NK 1_1NZ03.0001.fcs · NZ2017-04-21F9Q23 + NK 1_1NZ04.0001.fcs · NZ2017-04-21G9Q23 + NK 1_1NZ05.0001.fcs · NZ2017-04-21H9Q23 + NK 1_1NZ06.0001.fcs · NZ2017-05-01C9Q23 + NK 1_1NZ07.0001.fcs · NZ2017-05-01D9Q23 + NK 1_1NZ08.0001.fcs · NZ2017-05-01E9Q23 + NK 1_1NZ09.0001.fcs · NZ2017-05-01F9Q23 + NK 1_1NZ11.0001.fcs · NZ2017-05-01G9Q23 + NK 1_1NZ13.0001.fcs · NZ2017-05-01H9Q23 + NK 1_1NZ14.0001.fcs |
| · NK cells were co-cultured with autologous HIV-infected CD4+ T cells at effector-to-target ratio 1:4 | NZ2017-04-21C8Q23 + NK 1_4NZ01.0001.fcs · NZ2017-04-21D8Q23 + NK 1_4NZ02.0001.fcs · NZ2017-04-21E8Q23 + NK 1_4NZ03.0001.fcs · NZ2017-04-21F8Q23 + NK 1_4NZ04.0001.fcs · NZ2017-04-21G8Q23 + NK 1_4NZ05.0001.fcs · NZ2017-04-21H8Q23 + NK 1_4NZ06.0001.fcs · NZ2017-05-01C8Q23 + NK 1_4NZ07.0001.fcs · NZ2017-05-01D8Q23 + NK 1_4NZ08.0001.fcs · NZ2017-05-01E8Q23 + NK 1_4NZ09.0001.fcs · NZ2017-05-01F8Q23 + NK 1_4NZ11.0001.fcs · NZ2017-05-01G8Q23 + NK 1_4NZ13.0001.fcs · NZ2017-05-01H8Q23 + NK 1_4NZ14.0001.fcs |
| · NK cells were co-cultured with autologous mock-infected CD4+ T cells at effector-to-target ratio 4:1 | NZ2017-04-21C5UI + NK 4_1NZ01.0001.fcs · NZ2017-04-21D5UI + NK 4_1NZ02.0001.fcs · NZ2017-04-21E5UI + NK 4_1NZ03.0001.fcs · NZ2017-04-21F5UI + NK 4_1NZ04.0001.fcs · NZ2017-04-21G5UI + NK 4_1NZ05.0001.fcs · NZ2017-04-21H5UI + NK 4_1NZ06.0001.fcs · NZ2017-05-01C5UI + NK 4_1NZ07.0001.fcs · NZ2017-05-01D5UI + NK 4_1NZ08.0001.fcs · NZ2017-05-01E5UI + NK 4_1NZ09.0001.fcs · NZ2017-05-01F5UI + NK 4_1NZ11.0001.fcs · NZ2017-05-01G5UI + NK 4_1NZ13.0001.fcs · NZ2017-05-01H5UI + NK 4_1NZ14.0001.fcs |
| · NK cells were co-cultured with autologous mock-infected CD4+ T cells at effector-to-target ratio 1:1 | NZ2017-04-21C4UI + NK 1_1NZ01.0001.fcs · NZ2017-04-21D4UI + NK 1_1NZ02.0001.fcs · NZ2017-04-21E4UI + NK 1_1NZ03.0001.fcs · NZ2017-04-21F4UI + NK 1_1NZ04.0001.fcs · NZ2017-04-21G4UI + NK 1_1NZ05.0001.fcs · NZ2017-04-21H4UI + NK 1_1NZ06.0001.fcs · NZ2017-05-01C4UI + NK 1_1NZ07.0001.fcs · NZ2017-05-01D4UI + NK 1_1NZ08.0001.fcs · NZ2017-05-01E4UI + NK 1_1NZ09.0001.fcs · NZ2017-05-01F4UI + NK 1_1NZ11.0001.fcs · NZ2017-05-01G4UI + NK 1_1NZ13.0001.fcs · NZ2017-05-01H4UI + NK 1_1NZ14.0001.fcs |
| · NK cells were co-cultured with autologous mock-infected CD4+ T cells at effector-to-target ratio 1:4 | NZ2017-04-21C3UI + NK 1_4NZ01.0001.fcs · NZ2017-04-21D3UI + NK 1_4NZ02.0001.fcs · NZ2017-04-21E3UI + NK 1_4NZ03.0001.fcs · NZ2017-04-21F3UI + NK 1_4NZ04.0001.fcs · NZ2017-04-21G3UI + NK 1_4NZ05.0001.fcs · NZ2017-04-21H3UI + NK 1_4NZ06.0001.fcs · NZ2017-05-01C3UI + NK 1_4NZ07.0001.fcs · NZ2017-05-01D3UI + NK 1_4NZ08.0001.fcs · NZ2017-05-01E3UI + NK 1_4NZ09.0001.fcs · NZ2017-05-01F3UI + NK 1_4NZ11.0001.fcs · NZ2017-05-01G3UI + NK 1_4NZ13.0001.fcs · NZ2017-05-01H3UI + NK 1_4NZ14.0001.fcs |
| · NK cells were cultured alone | NZ2017-04-21C12NKNZ01.0001.fcs · NZ2017-04-21D12NKNZ02.0001.fcs · NZ2017-04-21E12NKNZ03.0001.fcs · NZ2017-04-21F12NKNZ04.0001.fcs · NZ2017-04-21G12NKNZ05.0001.fcs · NZ2017-04-21H12NKNZ06.0001.fcs · NZ2017-05-01C12NKNZ07.0001.fcs · NZ2017-05-01D12NKNZ08.0001.fcs · NZ2017-05-01E12NKNZ09.0001.fcs · NZ2017-05-01F12NKNZ11.0001.fcs · NZ2017-05-01G12NKNZ13.0001.fcs · NZ2017-05-01H12NKNZ14.0001.fcs |
| · HIV-infected CD4+ T cells were cultured alone | NZ2017-04-21C7Q23NZ01.0001.fcs · NZ2017-04-21D7Q23NZ02.0001.fcs · NZ2017-04-21E7Q23NZ03.0001.fcs · NZ2017-04-21F7Q23NZ04.0001.fcs · NZ2017-04-21G7Q23NZ05.0001.fcs · NZ2017-04-21H7Q23NZ06.0001.fcs · NZ2017-05-01C7Q23NZ07.0001.fcs · NZ2017-05-01D7Q23NZ08.0001.fcs · NZ2017-05-01E7Q23NZ09.0001.fcs · NZ2017-05-01F7Q23NZ11.0001.fcs · NZ2017-05-01G7Q23NZ13.0001.fcs · NZ2017-05-01H7Q23NZ14.0001.fcs |
| · Mock-infected CD4+ T cells were cultured alone | NZ2017-04-21C2UINZ01.0001.fcs · NZ2017-04-21D2UINZ02.0001.fcs · NZ2017-04-21E2UINZ03.0001.fcs · NZ2017-04-21F2UINZ04.0001.fcs · NZ2017-04-21G2UINZ05.0001.fcs · NZ2017-04-21H2UINZ06.0001.fcs · NZ2017-05-01C2UINZ07.0001.fcs · NZ2017-05-01D2UINZ08.0001.fcs · NZ2017-05-01E2UINZ09.0001.fcs · NZ2017-05-01F2UINZ11.0001.fcs · NZ2017-05-01G2UINZ13.0001.fcs · NZ2017-05-01H2UINZ14.0001.fcs |
| Individuals | |
|---|---|
| · Donor NZ01 | NZ2017-04-21C10Q23 + NK 4_1NZ01.0001.fcs · NZ2017-04-21C12NKNZ01.0001.fcs · NZ2017-04-21C2UINZ01.0001.fcs · NZ2017-04-21C3UI + NK 1_4NZ01.0001.fcs · NZ2017-04-21C4UI + NK 1_1NZ01.0001.fcs · NZ2017-04-21C5UI + NK 4_1NZ01.0001.fcs · NZ2017-04-21C7Q23NZ01.0001.fcs · NZ2017-04-21C8Q23 + NK 1_4NZ01.0001.fcs · NZ2017-04-21C9Q23 + NK 1_1NZ01.0001.fcs |
| · Donor NZ02 | NZ2017-04-21D10Q23 + NK 4_1NZ02.0001.fcs · NZ2017-04-21D12NKNZ02.0001.fcs · NZ2017-04-21D2UINZ02.0001.fcs · NZ2017-04-21D3UI + NK 1_4NZ02.0001.fcs · NZ2017-04-21D4UI + NK 1_1NZ02.0001.fcs · NZ2017-04-21D5UI + NK 4_1NZ02.0001.fcs · NZ2017-04-21D7Q23NZ02.0001.fcs · NZ2017-04-21D8Q23 + NK 1_4NZ02.0001.fcs · NZ2017-04-21D9Q23 + NK 1_1NZ02.0001.fcs |
| · Donor NZ03 | NZ2017-04-21E10Q23 + NK 4_1NZ03.0001.fcs · NZ2017-04-21E12NKNZ03.0001.fcs · NZ2017-04-21E2UINZ03.0001.fcs · NZ2017-04-21E3UI + NK 1_4NZ03.0001.fcs · NZ2017-04-21E4UI + NK 1_1NZ03.0001.fcs · NZ2017-04-21E5UI + NK 4_1NZ03.0001.fcs · NZ2017-04-21E7Q23NZ03.0001.fcs · NZ2017-04-21E8Q23 + NK 1_4NZ03.0001.fcs · NZ2017-04-21E9Q23 + NK 1_1NZ03.0001.fcs |
| · Donor NZ04 | NZ2017-04-21F10Q23 + NK 4_1NZ04.0001.fcs · NZ2017-04-21F12NKNZ04.0001.fcs · NZ2017-04-21F2UINZ04.0001.fcs · NZ2017-04-21F3UI + NK 1_4NZ04.0001.fcs · NZ2017-04-21F4UI + NK 1_1NZ04.0001.fcs · NZ2017-04-21F5UI + NK 4_1NZ04.0001.fcs · NZ2017-04-21F7Q23NZ04.0001.fcs · NZ2017-04-21F8Q23 + NK 1_4NZ04.0001.fcs · NZ2017-04-21F9Q23 + NK 1_1NZ04.0001.fcs |
| · Donor NZ05 | NZ2017-04-21G10Q23 + NK 4_1NZ05.0001.fcs · NZ2017-04-21G12NKNZ05.0001.fcs · NZ2017-04-21G2UINZ05.0001.fcs · NZ2017-04-21G3UI + NK 1_4NZ05.0001.fcs · NZ2017-04-21G4UI + NK 1_1NZ05.0001.fcs · NZ2017-04-21G5UI + NK 4_1NZ05.0001.fcs · NZ2017-04-21G7Q23NZ05.0001.fcs · NZ2017-04-21G8Q23 + NK 1_4NZ05.0001.fcs · NZ2017-04-21G9Q23 + NK 1_1NZ05.0001.fcs |
| · Donor NZ06 | NZ2017-04-21H10Q23 + NK 4_1NZ06.0001.fcs · NZ2017-04-21H12NKNZ06.0001.fcs · NZ2017-04-21H2UINZ06.0001.fcs · NZ2017-04-21H3UI + NK 1_4NZ06.0001.fcs · NZ2017-04-21H4UI + NK 1_1NZ06.0001.fcs · NZ2017-04-21H5UI + NK 4_1NZ06.0001.fcs · NZ2017-04-21H7Q23NZ06.0001.fcs · NZ2017-04-21H8Q23 + NK 1_4NZ06.0001.fcs · NZ2017-04-21H9Q23 + NK 1_1NZ06.0001.fcs |
| · Donor NZ07 | NZ2017-05-01C10Q23 + NK 4_1NZ07.0001.fcs · NZ2017-05-01C12NKNZ07.0001.fcs · NZ2017-05-01C2UINZ07.0001.fcs · NZ2017-05-01C3UI + NK 1_4NZ07.0001.fcs · NZ2017-05-01C4UI + NK 1_1NZ07.0001.fcs · NZ2017-05-01C5UI + NK 4_1NZ07.0001.fcs · NZ2017-05-01C7Q23NZ07.0001.fcs · NZ2017-05-01C8Q23 + NK 1_4NZ07.0001.fcs · NZ2017-05-01C9Q23 + NK 1_1NZ07.0001.fcs |
| · Donor NZ08 | NZ2017-05-01D10Q23 + NK 4_1NZ08.0001.fcs · NZ2017-05-01D12NKNZ08.0001.fcs · NZ2017-05-01D2UINZ08.0001.fcs · NZ2017-05-01D3UI + NK 1_4NZ08.0001.fcs · NZ2017-05-01D4UI + NK 1_1NZ08.0001.fcs · NZ2017-05-01D5UI + NK 4_1NZ08.0001.fcs · NZ2017-05-01D7Q23NZ08.0001.fcs · NZ2017-05-01D8Q23 + NK 1_4NZ08.0001.fcs · NZ2017-05-01D9Q23 + NK 1_1NZ08.0001.fcs |
| · Donor NZ09 | NZ2017-05-01E10Q23 + NK 4_1NZ09.0001.fcs · NZ2017-05-01E12NKNZ09.0001.fcs · NZ2017-05-01E2UINZ09.0001.fcs · NZ2017-05-01E3UI + NK 1_4NZ09.0001.fcs · NZ2017-05-01E4UI + NK 1_1NZ09.0001.fcs · NZ2017-05-01E5UI + NK 4_1NZ09.0001.fcs · NZ2017-05-01E7Q23NZ09.0001.fcs · NZ2017-05-01E8Q23 + NK 1_4NZ09.0001.fcs · NZ2017-05-01E9Q23 + NK 1_1NZ09.0001.fcs |
| · Donor NZ11 | NZ2017-05-01F10Q23 + NK 4_1NZ11.0001.fcs · NZ2017-05-01F12NKNZ11.0001.fcs · NZ2017-05-01F2UINZ11.0001.fcs · NZ2017-05-01F3UI + NK 1_4NZ11.0001.fcs · NZ2017-05-01F4UI + NK 1_1NZ11.0001.fcs · NZ2017-05-01F5UI + NK 4_1NZ11.0001.fcs · NZ2017-05-01F7Q23NZ11.0001.fcs · NZ2017-05-01F8Q23 + NK 1_4NZ11.0001.fcs · NZ2017-05-01F9Q23 + NK 1_1NZ11.0001.fcs |
| · Donor NZ13 | NZ2017-05-01G10Q23 + NK 4_1NZ13.0001.fcs · NZ2017-05-01G12NKNZ13.0001.fcs · NZ2017-05-01G2UINZ13.0001.fcs · NZ2017-05-01G3UI + NK 1_4NZ13.0001.fcs · NZ2017-05-01G4UI + NK 1_1NZ13.0001.fcs · NZ2017-05-01G5UI + NK 4_1NZ13.0001.fcs · NZ2017-05-01G7Q23NZ13.0001.fcs · NZ2017-05-01G8Q23 + NK 1_4NZ13.0001.fcs · NZ2017-05-01G9Q23 + NK 1_1NZ13.0001.fcs |
| · Donor NZ14 | NZ2017-05-01H10Q23 + NK 4_1NZ14.0001.fcs · NZ2017-05-01H12NKNZ14.0001.fcs · NZ2017-05-01H2UINZ14.0001.fcs · NZ2017-05-01H3UI + NK 1_4NZ14.0001.fcs · NZ2017-05-01H4UI + NK 1_1NZ14.0001.fcs · NZ2017-05-01H5UI + NK 4_1NZ14.0001.fcs · NZ2017-05-01H7Q23NZ14.0001.fcs · NZ2017-05-01H8Q23 + NK 1_4NZ14.0001.fcs · NZ2017-05-01H9Q23 + NK 1_1NZ14.0001.fcs |
