Experiment Overview
| Repository ID: | FR-FCM-Z7HM | Experiment name: | ZTP Random Loop | MIFlowCyt score: | 23.25% |
| Primary researcher: | David Bushhouse | PI/manager: | Julius Lucks | Uploaded by: | David Bushhouse |
| Experiment dates: | 2023-02-19 - 2023-08-06 | Dataset uploaded: | May 2024 | Last updated: | Oct 2024 |
| Keywords: | [Synthetic Biology] [riboswitches] [transcription] | Manuscripts: | |||
| Organizations: |
Northwestern University, Evanston, IL (USA)
|
||||
| Purpose: | Characterization of the tradeoff between sensitivity and dynamic range mediated by the sequence of the Cbe pfl ZTP riboswitch terminator loop. A library of riboswitch GFP reporter plasmids with randomized loop regions was characterized by FACS-seq in the presence of different concentrations of AICAR, allowing the construction of dose-response curves for hundreds of loop variants. These results include 2 replicate experiments in which the library fluorescence distribution was characterized in different concentrations AICAR. Sequencing data for this FACS-seq assay is available at SRA accession SUB14309952. | ||||
| Conclusion: | None | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
