Experiment Overview
| Repository ID: | FR-FCM-Z7Y7 | Experiment name: | Deciphering bone marrow engraftment after allo-HSCT in humans using single cell analyses. | MIFlowCyt score: | 65.75% |
| Primary researcher: | Jennifer Bordenave | PI/manager: | Jennifer Bordenave | Uploaded by: | Jennifer Bordenave |
| Experiment dates: | 2021-01-15 - 2022-03-04 | Dataset uploaded: | Nov 2023 | Last updated: | Dec 2023 |
| Keywords: | [GvHD] [myeloid dendritic cells] [T lymphocytes] [B lymphocytes] [hematopoietic stem cells (HSCs)] | Manuscripts: | [39207851] [PMC11473149] | ||
| Organizations: |
Institut de Recherche Saint-Louis INSERM U976, Paris, (France)
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| Purpose: | The objective of the B-REC project is to investigate the mechanisms responsible for the delayed recovery of humoral immune responses after allogeneic hematopoietic stem cell transplantation. Specifically, this project will aim to provide an exhaustive characterization of regenerative hematopoiesis and B cell reconstitution in transplanted adult patients. The B-REC project should help to better define the immune status of transplant recipients, orient their therapeutic management and lay the foundations for novel therapeutic interventions. | ||||
| Conclusion: | Our results clearly illustrated that profound alterations in all B-cell and myeloid progenitors that characterize engraftment 3 months after HSCT are affected by many parameters, the main one being previous GVHD (reflected by two patient populations R1 and R2). Analyses of T-cell subsets also revealed alterations in subsets, some of which associated with markers of T-cell exhaustion. | ||||
| Comments: | two panels Panel Bone Marrow Panel PBMC | ||||
| Funding: | Institut National du Cancer | ||||
| Quality control: | provided files were normalized (using EQ beads) | ||||
Experiment variables
| Conditions | |
|---|---|
| · donor PBMC | 20210115_D13_Jennifer_Sang_01_1.fcs · 20210115_D14_Jennifer_Sang_01_1.fcs · 20210216_D15_PBMC_05_1.fcs · 20210216_D16_PBMC_05_1.fcs · 20210326_D18_PBMC_01_1.fcs · 20210419_D20_PBMC_01_1.fcs · 20210420_D21_PBMC_01_1.fcs · 20210421_D24_PBMC_01_1.fcs · 20210520_D32_PBMC_02_0.fcs · 20210623_D38_PBMC_05_1.fcs · 20210624_D39_PBMC_01_1.fcs · 20210624_D43_PBMC_01_1.fcs · 20220215_D53_PBMC_Processed.fcs · 20220218_D52_PBMC_Processed.fcs · 20220218_D60_PBMC_Processed.fcs · D46_PBMC_corrige_modified_Processed_corrected.fcs |
| · recipient 3 months post-transplantation (PBMC) | 20210519_R4_J100_PBMC_03_1.fcs · 20210519_R5_J100_PBMC_03_1.fcs · 20210622_R15_J100_PBMC_04_1.fcs · 20210929_R39_J100_PBMC_01_1.fcs · 20210930_R21_J100_PBMC_01_1.fcs · 20210930_R38_J100_PBMC_01_1.fcs · 20211001_R20_J100_PBMC_01_1.fcs · 20211001_R44_J100_PBMC_01_1.fcs · 20220215_R42_J100_PBMC_Processed.fcs · 20220218_R59_J100_PBMC_Processed.fcs |
| · recipient 6 months post-transplantation (PBMC) | 20210929_R18_J6mois_PBMC_01_1.fcs · 20210929_R20_J6mois_PBMC_01_1.fcs · 20210930_R15_J6mois_PBMC_01_1.fcs · 20210930_R19_J6mois_PBMC_01_1.fcs · 20210930_R4_J6mois_PBMC_01_1.fcs · 20210930_R5_J6mois_PBMC_01_1.fcs · 20220215_R24_J6mois_PBMC_Processed.fcs · 20220215_R32_J6mois_PBMC_Processed.fcs · 20220215_R38_J6mois_PBMC_Processed.fcs · 20220215_R39_J6mois_PBMC_Processed.fcs · 20220218_R43_J6mois_PBMC_Processed.fcs |
| · Donor Bone Marrow | export_20200723_D1_01_1_CD15-.fcs · export_20200723_D2_01_1_CD15-.fcs · export_20200929_D3_01_1_CD15-.fcs · export_20200929_D4_01_1_CD15-.fcs · export_20200929_D5_01_1_CD15-.fcs · export_20200929_D6_01_1_CD15-.fcs · export_20201112_D10_02_1_CD15-.fcs · export_20201112_D7_02_1_CD15-.fcs · export_20201112_D8_02_1_CD15-.fcs · export_20201112_D9_02_1_CD15-.fcs · export_20210115_D11_Jennifer_01_1_CD15-.fcs · export_20210325_D01_MO_01_1_CD15-.fcs |
| · Recipient 3 months post-transplantation (Bone Marrow) | export_20210115_R1_MO_Jennifer_01_1_CD15-.fcs · export_20210118_R3_MO_Jennifer_01_1_CD15-.fcs · export_20210118_R6_MO_Jennifer_01_1_CD15-.fcs · export_20210118_R7_MO_Jennifer_01_1_CD15-.fcs · export_20210127_R12_MO_01_1_CD15-.fcs · export_20210127_R9_MO_01_1_CD15-.fcs · export_20210217_R14_MO_01_0_CD15-.fcs · export_20210325_R27_MO_01_1_CD15-.fcs · export_20210325_R28_MO_01_1_CD15-.fcs · export_20210325_R29_MO_01_1_CD15-.fcs · export_20210329_R23_MO_01_1_CD15-.fcs · export_20210420_R30_MO_01_1_CD15-.fcs · export_20210517_R31_MO_07_1_CD15-.fcs · export_20210517_R33_MO_07_1_CD15-.fcs · export_20210517_R35_MO_07_1_CD15-.fcs · export_20210622_R15_MO_03_1_CD15-.fcs · export_20210622_R36_MO_04_1_CD15-.fcs · export_20210622_R41_MO_04_1_CD15-.fcs · export_20210928_R21_MO_01_1_CD15-.fcs · export_20210928_R47_MO_01_1_CD15-.fcs · export_20210928_R48_MO_01_1_CD15-.fcs · export_20210928_R50_MO_01_1_CD15-.fcs · export_20220214_R42_J100_MO_Processed_CD15-.fcs · export_20220214_R58_MO_Processed_CD15-.fcs · export_20220214_R59_J100_MO_Processed_Concatenated_CD15-.fcs · export_20220304_R54_MO_Processed_CD15-.fcs · export_20220304_R60_MO_Processed_CD15-.fcs |
| · Recipient panel T cells 3 months post-transplantation (Bone Marrow) | export_20210927_R12_MO_01_1_beads.fcs · export_20210927_R14_MO_01_1_beads.fcs · export_20210927_R28_MO_01_1_beads.fcs · export_20210927_R31_MO_conca_beads.fcs · export_20210927_R33_MO_01_1_beads.fcs · export_20210927_R6_MO_01_1_beads.fcs · export_20220218_R30_MO_Processed_beads.fcs · export_20220218_R50_MO_Processed_beads.fcs |
| · Donor Panel T cells | export_20210927_D4_MO_01_1_beads.fcs · export_20210927_D6_MO_conca_beads.fcs · export_20210927_D8_MO_01_1_beads.fcs · export_20220218_D3_MO_Processed_beads.fcs · export_20220218_D5_MO_Processed_beads.fcs · export_20220218_D7_MO_Processed_beads.fcs |
