Experiment Overview
| Repository ID: | FR-FCM-Z873 | Experiment name: | Effect of hydrogen peroxide on the salivary microbiome | MIFlowCyt score: | 36.00% |
| Primary researcher: | Fabian Mermans | PI/manager: | Nico Boon | Uploaded by: | Fabian Mermans |
| Experiment dates: | 2022-08-01 - 2023-11-30 | Dataset uploaded: | Aug 2024 | Last updated: | Aug 2024 |
| Keywords: | [FACS] [cell sorting] [PMA] [saliva] [oral microbiome] [H2O2] [PMAxx] [oral microbiota] | Manuscripts: | |||
| Organizations: |
Ghent University, Center for Microbial Ecology and Technology, Ghent, (Belgium)
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| Purpose: | To assess the effect of hydrogen peroxide on the salivary microbiome | ||||
| Conclusion: | Hydrogen peroxide causes a shift in microbiome composition | ||||
| Comments: | None | ||||
| Funding: | This work was supported by Research Foundation – Flanders (FWO, Belgium) (grant number G0B2719N) | ||||
| Quality control: | Daily QC of flow cytometer - Biological triplicates - Axenic control samples - Medium blancs - Dilutant blancs - Singlet analysis - Stability fluorescent signal over time | ||||
Experiment variables
| Conditions | |
|---|---|
| · Control | 20230619_Fabian_H2O2_PMA_Controls_SGPI_1_PBS_H2O2_newPBS.fcs · 20230619_Fabian_H2O2_PMA_Controls_SGPI_1_PBS_Raw.fcs · 20230621_Fabian_H2O2_PMA_Controls_SGPI_1000_FilteredSaliva.fcs · 20230621_Fabian_H2O2_PMA_Controls_SGPI_100_FilteredSaliva.fcs · 20230621_Fabian_H2O2_PMA_Controls_SGPI_1_PBS.fcs |
| · Untreated | 20230619_Fabian_H2O2_PMA_Raw_SGPI_1010_D1.fcs · 20230619_Fabian_H2O2_PMA_Raw_SGPI_1010_D2.fcs · 20230619_Fabian_H2O2_PMA_Raw_SGPI_1010_D3.fcs · 20230619_Fabian_H2O2_PMA_Raw_SGPI_1010_D4.fcs · 20230619_Fabian_H2O2_PMA_Raw_SGPI_1010_D5.fcs · 20230621_Fabian_H2O2_PMA_Raw_SGPI_1000_D10.fcs · 20230621_Fabian_H2O2_PMA_Raw_SGPI_1000_D6.fcs · 20230621_Fabian_H2O2_PMA_Raw_SGPI_1000_D7.fcs · 20230621_Fabian_H2O2_PMA_Raw_SGPI_1000_D8.fcs · 20230621_Fabian_H2O2_PMA_Raw_SGPI_1000_D9.fcs |
| · H2O2-treated | 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D1_R1.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D1_R2.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D1_R3.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D2_R1.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D2_R2.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D2_R3.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D3_R1.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D3_R2.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D3_R3.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D4_R1.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D4_R2.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D4_R3.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D5_R1.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D5_R2.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D5_R3.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D10_R1.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D10_R2.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D10_R3.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D6_R1.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D6_R2.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D6_R3.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D7_R1.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D7_R2.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D7_R3.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D8_R1.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D8_R2.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D8_R3.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D9_R1.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D9_R2.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D9_R3.fcs |
| Individuals | |
|---|---|
| · Control | 20230619_Fabian_H2O2_PMA_Controls_SGPI_1_PBS_H2O2_newPBS.fcs · 20230619_Fabian_H2O2_PMA_Controls_SGPI_1_PBS_Raw.fcs · 20230621_Fabian_H2O2_PMA_Controls_SGPI_1000_FilteredSaliva.fcs · 20230621_Fabian_H2O2_PMA_Controls_SGPI_100_FilteredSaliva.fcs · 20230621_Fabian_H2O2_PMA_Controls_SGPI_1_PBS.fcs |
| · D1 | 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D1_R1.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D1_R2.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D1_R3.fcs · 20230619_Fabian_H2O2_PMA_Raw_SGPI_1010_D1.fcs |
| · D2 | 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D2_R1.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D2_R2.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D2_R3.fcs · 20230619_Fabian_H2O2_PMA_Raw_SGPI_1010_D2.fcs |
| · D3 | 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D3_R1.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D3_R2.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D3_R3.fcs · 20230619_Fabian_H2O2_PMA_Raw_SGPI_1010_D3.fcs |
| · D4 | 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D4_R1.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D4_R2.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D4_R3.fcs · 20230619_Fabian_H2O2_PMA_Raw_SGPI_1010_D4.fcs |
| · D5 | 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D5_R1.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D5_R2.fcs · 20230619_Fabian_H2O2_PMA_H2O2_SGPI_1000_D5_R3.fcs · 20230619_Fabian_H2O2_PMA_Raw_SGPI_1010_D5.fcs |
| · D6 | 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D6_R1.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D6_R2.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D6_R3.fcs · 20230621_Fabian_H2O2_PMA_Raw_SGPI_1000_D6.fcs |
| · D7 | 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D7_R1.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D7_R2.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D7_R3.fcs · 20230621_Fabian_H2O2_PMA_Raw_SGPI_1000_D7.fcs |
| · D8 | 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D8_R1.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D8_R2.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D8_R3.fcs · 20230621_Fabian_H2O2_PMA_Raw_SGPI_1000_D8.fcs |
| · D9 | 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D9_R1.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D9_R2.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D9_R3.fcs · 20230621_Fabian_H2O2_PMA_Raw_SGPI_1000_D9.fcs |
| · D10 | 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D10_R1.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D10_R2.fcs · 20230621_Fabian_H2O2_PMA_PEROX_SGPI_1000_D10_R3.fcs · 20230621_Fabian_H2O2_PMA_Raw_SGPI_1000_D10.fcs |
