Experiment Overview
| Repository ID: | FR-FCM-Z8F2 | Experiment name: | Malaria naive in vitro stim | MIFlowCyt score: | 56.00% |
| Primary researcher: | Jessica Loughland | PI/manager: | Jessica Loughland | Uploaded by: | Jessica Loughland |
| Experiment dates: | 2020-01-06 - 2025-12-21 | Dataset uploaded: | Nov 2024 | Last updated: | Nov 2024 |
| Keywords: | [monocytes] [malaria] [innate] [gd T cells] [age] | Manuscripts: | |||
| Organizations: |
QIMR-Berghofer, QIMR-Berghofer, Kelvin Grove, QLD (Australia)
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| Purpose: | Here, we aimed to assess the cytokine production and activation of innate cells in malaria-naive children and adults. We stimulated PBMCs from malaria-naive children and adults with Plasmodium-infected red blodd cells in vitro. | ||||
| Conclusion: | There are differences between how innate cells from children and adults respond to a first parasite exposure | ||||
| Comments: | None | ||||
| Funding: | This work was supported by the National Health and Medical Research Council of Australia (program grant 1132975 to C.R.E. and N.M.A); Senior Research Fellowships C.R.E. (1154265) and NMA (1135820), Career Development Award 1141278, Project Grant 1125656, and Ideas Grant 1181932 to M.J.B.; Program Grant 290208, Emerging Leadership 2 Fellowships to BEB and MJG); the CSL Centenary Fellowship to M.J.B, and the Snow Medical Foundation Fellowship 2022/SF167 to M.J.B. The Burnet Institute is supported by the NHMRC for Independent Research Institutes Infrastructure Support Scheme and the Victorian State Government Operational Infrastructure Support. | ||||
| Quality control: | We used uninfected red blood cells as a control in our in vitro experiments | ||||
Experiment variables
| Sample Type | |
|---|---|
| · T cell activation assay | Experiment_005-Tfh_pRBC-004_Unmixed.fcs · Experiment_005-Tfh_pRBC-005_Unmixed.fcs · Experiment_005-Tfh_pRBC-007_Unmixed.fcs · Experiment_005-Tfh_pRBC-008_Unmixed.fcs · Experiment_005-Tfh_pRBC-011_Unmixed.fcs · Experiment_005-Tfh_pRBC-012_Unmixed.fcs · Experiment_005-Tfh_pRBC-018_Unmixed.fcs · Experiment_005-Tfh_pRBC-028_Unmixed.fcs · Experiment_005-Tfh_pRBC-029_Unmixed.fcs · Experiment_005-Tfh_pRBC-030_Unmixed.fcs · Experiment_005-Tfh_pRBC-032_Unmixed.fcs · Experiment_005-Tfh_pRBC-043_Unmixed.fcs · Experiment_005-Tfh_pRBC-044_Unmixed.fcs · Experiment_005-Tfh_uRBC-004_Unmixed.fcs · Experiment_005-Tfh_uRBC-005_Unmixed.fcs · Experiment_005-Tfh_uRBC-007_Unmixed.fcs · Experiment_005-Tfh_uRBC-008_Unmixed.fcs · Experiment_005-Tfh_uRBC-011_Unmixed.fcs · Experiment_005-Tfh_uRBC-012_Unmixed.fcs · Experiment_005-Tfh_uRBC-018_Unmixed.fcs · Experiment_005-Tfh_uRBC-028_Unmixed.fcs · Experiment_005-Tfh_uRBC-029_Unmixed.fcs · Experiment_005-Tfh_uRBC-030_Unmixed.fcs · Experiment_005-Tfh_uRBC-032_Unmixed.fcs · Experiment_005-Tfh_uRBC-043_Unmixed.fcs · Experiment_005-Tfh_uRBC-044_Unmixed.fcs |
| · PBMC phenotyping | Age_Phenotyping_29.8-Age_001_Unmixed.fcs · Age_Phenotyping_29.8-Age_002_Unmixed.fcs · Age_Phenotyping_29.8-Age_003_Unmixed.fcs · Age_Phenotyping_29.8-Age_005_Unmixed.fcs · Age_Phenotyping_29.8-Age_007_Unmixed.fcs · Age_Phenotyping_29.8-Age_008_Unmixed.fcs · Age_Phenotyping_29.8-Age_011_Unmixed.fcs · Age_Phenotyping_29.8-Age_012_Unmixed.fcs · Age_Phenotyping_29.8-Age_018_Unmixed.fcs · Age_Phenotyping_29.8-Age_023_Unmixed.fcs · Age_Phenotyping_29.8-Age_024_Unmixed.fcs · Age_Phenotyping_29.8-Age_025_Unmixed.fcs · Age_Phenotyping_29.8-Age_026_Unmixed.fcs · Age_Phenotyping_29.8-Age_30_Unmixed.fcs · Age_Phenotyping_29.8-Age_31_Unmixed.fcs · Age_Phenotyping_29.8-Age_32_Unmixed.fcs · Age_Phenotyping_29.8-Age_33_Unmixed.fcs · Age_Phenotyping_29.8-Age_34_Unmixed.fcs · Age_Phenotyping_29.8-Age_35_Unmixed.fcs · Age_Phenotyping_29.8-Age_39_Unmixed.fcs · Age_Phenotyping_29.8-Age_41_Unmixed.fcs · Age_Phenotyping_29.8-Age_42_Unmixed.fcs · Age_Phenotyping_29.8-Age_43_Unmixed.fcs · Age_Phenotyping_29.8-Age_44_Unmixed.fcs |
| · Innate cell cytokine assay | Experiment_005-pRBC-Innate_018_Unmixed.fcs · Experiment_005-pRBC-Innate_023_Unmixed.fcs · Experiment_005-pRBC-Innate_024_Unmixed.fcs · Experiment_005-pRBC-Innate_025_Unmixed.fcs · Experiment_005-pRBC-Innate_026_Unmixed.fcs · Experiment_005-pRBC-Innate_028_Unmixed.fcs · Experiment_005-pRBC-Innate_029_Unmixed.fcs · Experiment_005-pRBC-Innate_030_Unmixed.fcs · Experiment_005-pRBC-Innate_031_Unmixed.fcs · Experiment_005-pRBC-Innate_032_Unmixed.fcs · Experiment_005-pRBC-Innate_033_Unmixed.fcs · Experiment_005-pRBC-Innate_034_Unmixed.fcs · Experiment_005-pRBC-Innate_035_Unmixed.fcs · Experiment_005-pRBC-Innate_039_Unmixed.fcs · Experiment_005-uRBC-Innate_001_Unmixed.fcs · Experiment_005-uRBC-Innate_003_Unmixed.fcs · Experiment_005-uRBC-Innate_005_Unmixed.fcs · Experiment_005-pRBC-Innate_001_Unmixed.fcs · Experiment_005-pRBC-Innate_003_Unmixed.fcs · Experiment_005-pRBC-Innate_005_Unmixed.fcs · Experiment_005-pRBC-Innate_007_Unmixed.fcs · Experiment_005-pRBC-Innate_008_Unmixed.fcs · Experiment_005-pRBC-Innate_011_Unmixed.fcs · Experiment_005-uRBC-Innate_007_Unmixed.fcs · Experiment_005-uRBC-Innate_008_Unmixed.fcs · Experiment_005-uRBC-Innate_011_Unmixed.fcs · Experiment_005-uRBC-Innate_018_Unmixed.fcs · Experiment_005-uRBC-Innate_023_Unmixed.fcs · Experiment_005-uRBC-Innate_024_Unmixed.fcs · Experiment_005-uRBC-Innate_025_Unmixed.fcs · Experiment_005-uRBC-Innate_026_Unmixed.fcs · Experiment_005-uRBC-Innate_028_Unmixed.fcs · Experiment_005-uRBC-Innate_029_Unmixed.fcs · Experiment_005-uRBC-Innate_030_Unmixed.fcs · Experiment_005-uRBC-Innate_031_Unmixed.fcs · Experiment_005-uRBC-Innate_032_Unmixed.fcs · Experiment_005-uRBC-Innate_033_Unmixed.fcs · Experiment_005-uRBC-Innate_034_Unmixed.fcs · Experiment_005-uRBC-Innate_035_Unmixed.fcs · Experiment_005-uRBC-Innate_039_Unmixed.fcs · Experiment_005-uRBC-Innate_041_Unmixed.fcs · Experiment_005-uRBC-Innate_042_Unmixed.fcs · Experiment_005-uRBC-Innate_043_Unmixed.fcs · Experiment_005-uRBC-Innate_044_Unmixed.fcs · Experiment_005-pRBC-Innate_041_Unmixed.fcs · Experiment_005-pRBC-Innate_042_Unmixed.fcs · Experiment_005-pRBC-Innate_043_Unmixed.fcs · Experiment_005-pRBC-Innate_044_Unmixed.fcs |
| Conditions | |
|---|---|
| · 24 hr pRBC or uRBC stim | Experiment_005-pRBC-Innate_018_Unmixed.fcs · Experiment_005-pRBC-Innate_023_Unmixed.fcs · Experiment_005-pRBC-Innate_024_Unmixed.fcs · Experiment_005-pRBC-Innate_025_Unmixed.fcs · Experiment_005-pRBC-Innate_026_Unmixed.fcs · Experiment_005-pRBC-Innate_028_Unmixed.fcs · Experiment_005-pRBC-Innate_029_Unmixed.fcs · Experiment_005-pRBC-Innate_030_Unmixed.fcs · Experiment_005-pRBC-Innate_031_Unmixed.fcs · Experiment_005-pRBC-Innate_032_Unmixed.fcs · Experiment_005-pRBC-Innate_033_Unmixed.fcs · Experiment_005-pRBC-Innate_034_Unmixed.fcs · Experiment_005-pRBC-Innate_035_Unmixed.fcs · Experiment_005-pRBC-Innate_039_Unmixed.fcs · Experiment_005-uRBC-Innate_001_Unmixed.fcs · Experiment_005-uRBC-Innate_003_Unmixed.fcs · Experiment_005-uRBC-Innate_005_Unmixed.fcs · Experiment_005-pRBC-Innate_001_Unmixed.fcs · Experiment_005-pRBC-Innate_003_Unmixed.fcs · Experiment_005-pRBC-Innate_005_Unmixed.fcs · Experiment_005-pRBC-Innate_007_Unmixed.fcs · Experiment_005-pRBC-Innate_008_Unmixed.fcs · Experiment_005-pRBC-Innate_011_Unmixed.fcs · Experiment_005-uRBC-Innate_007_Unmixed.fcs · Experiment_005-uRBC-Innate_008_Unmixed.fcs · Experiment_005-uRBC-Innate_011_Unmixed.fcs · Experiment_005-uRBC-Innate_018_Unmixed.fcs · Experiment_005-uRBC-Innate_023_Unmixed.fcs · Experiment_005-uRBC-Innate_024_Unmixed.fcs · Experiment_005-uRBC-Innate_025_Unmixed.fcs · Experiment_005-uRBC-Innate_026_Unmixed.fcs · Experiment_005-uRBC-Innate_028_Unmixed.fcs · Experiment_005-uRBC-Innate_029_Unmixed.fcs · Experiment_005-uRBC-Innate_030_Unmixed.fcs · Experiment_005-uRBC-Innate_031_Unmixed.fcs · Experiment_005-uRBC-Innate_032_Unmixed.fcs · Experiment_005-uRBC-Innate_033_Unmixed.fcs · Experiment_005-uRBC-Innate_034_Unmixed.fcs · Experiment_005-uRBC-Innate_035_Unmixed.fcs · Experiment_005-uRBC-Innate_039_Unmixed.fcs · Experiment_005-uRBC-Innate_041_Unmixed.fcs · Experiment_005-uRBC-Innate_042_Unmixed.fcs · Experiment_005-uRBC-Innate_043_Unmixed.fcs · Experiment_005-uRBC-Innate_044_Unmixed.fcs · Experiment_005-pRBC-Innate_041_Unmixed.fcs · Experiment_005-pRBC-Innate_042_Unmixed.fcs · Experiment_005-pRBC-Innate_043_Unmixed.fcs · Experiment_005-pRBC-Innate_044_Unmixed.fcs |
| · 5 day pRBC or uRBC PBMC stim | Experiment_005-Tfh_pRBC-004_Unmixed.fcs · Experiment_005-Tfh_pRBC-005_Unmixed.fcs · Experiment_005-Tfh_pRBC-007_Unmixed.fcs · Experiment_005-Tfh_pRBC-008_Unmixed.fcs · Experiment_005-Tfh_pRBC-011_Unmixed.fcs · Experiment_005-Tfh_pRBC-012_Unmixed.fcs · Experiment_005-Tfh_pRBC-018_Unmixed.fcs · Experiment_005-Tfh_pRBC-028_Unmixed.fcs · Experiment_005-Tfh_pRBC-029_Unmixed.fcs · Experiment_005-Tfh_pRBC-030_Unmixed.fcs · Experiment_005-Tfh_pRBC-032_Unmixed.fcs · Experiment_005-Tfh_pRBC-043_Unmixed.fcs · Experiment_005-Tfh_pRBC-044_Unmixed.fcs · Experiment_005-Tfh_uRBC-004_Unmixed.fcs · Experiment_005-Tfh_uRBC-005_Unmixed.fcs · Experiment_005-Tfh_uRBC-007_Unmixed.fcs · Experiment_005-Tfh_uRBC-008_Unmixed.fcs · Experiment_005-Tfh_uRBC-011_Unmixed.fcs · Experiment_005-Tfh_uRBC-012_Unmixed.fcs · Experiment_005-Tfh_uRBC-018_Unmixed.fcs · Experiment_005-Tfh_uRBC-028_Unmixed.fcs · Experiment_005-Tfh_uRBC-029_Unmixed.fcs · Experiment_005-Tfh_uRBC-030_Unmixed.fcs · Experiment_005-Tfh_uRBC-032_Unmixed.fcs · Experiment_005-Tfh_uRBC-043_Unmixed.fcs · Experiment_005-Tfh_uRBC-044_Unmixed.fcs |
| · ex vivo | Age_Phenotyping_29.8-Age_001_Unmixed.fcs · Age_Phenotyping_29.8-Age_002_Unmixed.fcs · Age_Phenotyping_29.8-Age_003_Unmixed.fcs · Age_Phenotyping_29.8-Age_005_Unmixed.fcs · Age_Phenotyping_29.8-Age_007_Unmixed.fcs · Age_Phenotyping_29.8-Age_008_Unmixed.fcs · Age_Phenotyping_29.8-Age_011_Unmixed.fcs · Age_Phenotyping_29.8-Age_012_Unmixed.fcs · Age_Phenotyping_29.8-Age_018_Unmixed.fcs · Age_Phenotyping_29.8-Age_023_Unmixed.fcs · Age_Phenotyping_29.8-Age_024_Unmixed.fcs · Age_Phenotyping_29.8-Age_025_Unmixed.fcs · Age_Phenotyping_29.8-Age_026_Unmixed.fcs · Age_Phenotyping_29.8-Age_30_Unmixed.fcs · Age_Phenotyping_29.8-Age_31_Unmixed.fcs · Age_Phenotyping_29.8-Age_32_Unmixed.fcs · Age_Phenotyping_29.8-Age_33_Unmixed.fcs · Age_Phenotyping_29.8-Age_34_Unmixed.fcs · Age_Phenotyping_29.8-Age_35_Unmixed.fcs · Age_Phenotyping_29.8-Age_39_Unmixed.fcs · Age_Phenotyping_29.8-Age_41_Unmixed.fcs · Age_Phenotyping_29.8-Age_42_Unmixed.fcs · Age_Phenotyping_29.8-Age_43_Unmixed.fcs · Age_Phenotyping_29.8-Age_44_Unmixed.fcs |
