Experiment Overview
| Repository ID: | FR-FCM-Z8HZ | Experiment name: | TAR-based biosensor for bacterial small RNA | MIFlowCyt score: | 59.25% |
| Primary researcher: | Tyler Simons | PI/manager: | Tyler Simons | Uploaded by: | Tyler Simons |
| Experiment dates: | 2024-10-10 - 2024-10-11 | Dataset uploaded: | Nov 2024 | Last updated: | Dec 2024 |
| Keywords: | [Bacteria] [RNA hybridization] [biosensor] [E.coli BL21(DE3)] | Manuscripts: | |||
| Organizations: |
University of Utah, Salt Lake City, (USA)
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| Purpose: | Application of the S1 bacterial conditional degron to construct a TAR-based sRNA biosensor. The sensor was tested as a standalone RNA and encoded in the 3' UTR of mVenus-S1 protein mRNA. Each RNA was tested in the presence or absence of oxyS sRNA target being overexpressed. | ||||
| Conclusion: | The biosensor is functional, but has high variance between biological replicates. The sRNA sensor performs best when encoded separately but is also functional as a single transcript system. | ||||
| Comments: | None | ||||
| Funding: | National Institutes of Health (NIH) | ||||
| Quality control: | Daily Performance test performed with Thermo Attune™ Performance Tracking Beads (Thermo #4449754). At least four biological replicates were used per construct and experimental condition. | ||||
