Experiment Overview
| Repository ID: | FR-FCM-Z99Q | Experiment name: | Stewart et al PLoS Biology | MIFlowCyt score: | 54.19% |
| Primary researcher: | Robert Orchard | PI/manager: | Robert Orchard | Uploaded by: | Robert Orchard |
| Experiment dates: | 2025-03-28 - 2025-03-31 | Dataset uploaded: | Mar 2025 | Last updated: | Apr 2025 |
| Keywords: | [annexin V] [TMEM30a] [duramycin] [cd300lf] | Manuscripts: | [39574648] | ||
| Organizations: |
The University of Texas Southwestern Medical Center, Dallas, Texas (USA)
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| Purpose: | To determine the amount of phosphatidylserine, phostphatidylethanolamine, and CD300lf on the cell surface | ||||
| Conclusion: | TMEM30a is required for the asymetrical distribution of phospholipids in the cell membrane and this does not impact the localization of CD300lf. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Experiments were repeated by multiple members of the laboratory and data is representative of at least three independent experiments | ||||
Experiment variables
| Sample Type | |
|---|---|
| · BV2 Cells | Fig1A_KO.fcs · Fig1A_WT.fcs · Fig1B_Duramycin_KO.fcs · Fig1B_Duramycin_WT.fcs · Fig1F_KO_EmptyVector.fcs · Fig1F_KO_Tmem30Express · Fig1F_WT_EmptyVector.fcs · Fig1F_WT_TMEM30aExpress.fcs · Fig2C_KO_CD300lf.fcs · Fig2C_KO_Vector.fcs · Fig2C_WT_CD300lf.fcs · Fig2C_WT_Vector.fcs · Fig3A_KO_TMEM30a.fcs · Fig3A_KO_TMEM30aK308E.fcs · Fig3A_KO_TMEM30aW260A.fcs · Fig3A_KO_Vector.Fcs · Fig3A_WT_Vector.fcs · Fig3B_KO_TMEM30aK308E_Duramycin.fcs · Fig3B_KO_TMEM30aW260A_Duramycin.fcs · Fig3B_KO_TMEM30a_Duramycin.fcs · Fig3B_KO_Vector_Duramycin.fcs · Fig3B_WT_Vector_Duramycin.fcs · Fig3C_KO_TMEM30aK308E_HA.fcs · Fig3C_KO_TMEM30aW260a_HA.fcs · Fig3C_KO_TMEM30a_HA.fcs · Fig3C_KO_Vector_HA_.fcs · Fig3C_WT_HA.fcs |
