Experiment Overview
| Repository ID: | FR-FCM-Z9YB | Experiment name: | Immune subsets in blood post-COVID19 | MIFlowCyt score: | 82.25% |
| Primary researcher: | Glenda Canderan | PI/manager: | Glenda Canderan | Uploaded by: | Glenda Canderan |
| Experiment dates: | 2025-02-11 - | Dataset uploaded: | Feb 2025 | Last updated: | Mar 2025 |
| Keywords: | [PBMC] [human] [COVID-19] | Manuscripts: | |||
| Organizations: |
University of Virginia, Charlottesville, Virginia (USA)
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| Purpose: | Perform broad immune monitoring post-COVID19 | ||||
| Conclusion: | Several T cells subsets were associated with restrictive lung disease post-COVID19 | ||||
| Comments: | Please refer to the manuscript for the complete flow cytometry panel details and staining protocol. | ||||
| Funding: | National Institutes of Health grant U01 AI100799 National Institutes of Health grant R21 AI138077 National Institutes of Health grant R56 AI178669 University of Virginia School of Medicine GAP award University of Virginia Global Infectious Diseases Institute award University of Virginia Manning COVID-19 Research Fund | ||||
| Quality control: | All spectral flow cytometry data were pre-processed by spectral unmixing with autofluorescence subtraction and spill-over correction (SpectroFlow, Cytek Biosciences, 508 Fremont, CA, USA). Removal of dead cells, debris doublets and atypical events (antibody aggregates) was performed by expert gating. Similarly, expert gating was used to pre-gate live cells. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · Post-1 | COVID-RC_001_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_007_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_008_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_011_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_012_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_014_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_015_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_017_Post-1_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_019_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_021_Post-1_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_023_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_026_Post-1_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_027_Post-1_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_031_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_032_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_034_Post-1_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_037_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_039_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_046_Post-1_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_048_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_049_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_051_Post-1_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_052_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_057_Post-1_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_059_Post-1_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_061_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_062_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_064_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_068_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_069_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_077_Post-1_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_082_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_083_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_086_Post-1_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_088_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_097_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_100_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_101_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_105_Post-1_ImmuneSubsets_Batch-5_Live.fcs |
| · Post-2 | COVID-RC_009_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_017_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_021_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_024_Post-2_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_025_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_027_Post-2_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_028_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_030_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_037_Post-2_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_047_Post-2_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_051_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_057_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_059_Post-2_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_060_Post-2_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_065_Post-2_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_077_Post-2_ImmuneSubsets_Batch-4_Live.fcs |
| · Post-3 | COVID-RC_047_Post-3_ImmuneSubsets_Batch-4_Live.fcs |
| · NA | Healthy_014__ImmuneSubsets_Batch-3_Live.fcs · Healthy_028__ImmuneSubsets_Batch-3_Live.fcs · Healthy_044__ImmuneSubsets_Batch-3_Live.fcs · Healthy_122__ImmuneSubsets_Batch-3_Live.fcs · Healthy_140__ImmuneSubsets_Batch-4_Live.fcs · Healthy_154__ImmuneSubsets_Batch-1_Live.fcs · Healthy_155__ImmuneSubsets_Batch-1_Live.fcs · Healthy_156__ImmuneSubsets_Batch-1_Live.fcs · Healthy_157__ImmuneSubsets_Batch-2_Live.fcs · Healthy_159__ImmuneSubsets_Batch-2_Live.fcs · Healthy_162__ImmuneSubsets_Batch-4_Live.fcs · Healthy_163__ImmuneSubsets_Batch-1_Live.fcs · Healthy_164__ImmuneSubsets_Batch-2_Live.fcs · Healthy_168__ImmuneSubsets_Batch-4_Live.fcs · Healthy_172__ImmuneSubsets_Batch-4_Live.fcs · Healthy_175__ImmuneSubsets_Batch-1_Live.fcs · Healthy_180__ImmuneSubsets_Batch-2_Live.fcs · Healthy_183__ImmuneSubsets_Batch-4_Live.fcs |
| Sample Type | |
|---|---|
| · COVID-RC | COVID-RC_001_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_007_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_008_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_009_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_011_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_012_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_014_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_015_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_017_Post-1_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_017_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_019_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_021_Post-1_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_021_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_023_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_024_Post-2_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_025_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_026_Post-1_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_027_Post-1_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_027_Post-2_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_028_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_030_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_031_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_032_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_034_Post-1_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_037_Post-1_ImmuneSubsets_Batch-1_Live.fcs · COVID-RC_037_Post-2_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_039_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_046_Post-1_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_047_Post-2_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_047_Post-3_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_048_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_049_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_051_Post-1_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_051_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_052_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_057_Post-1_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_057_Post-2_ImmuneSubsets_Batch-2_Live.fcs · COVID-RC_059_Post-1_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_059_Post-2_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_060_Post-2_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_061_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_062_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_064_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_065_Post-2_ImmuneSubsets_Batch-3_Live.fcs · COVID-RC_068_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_069_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_077_Post-1_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_077_Post-2_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_082_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_083_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_086_Post-1_ImmuneSubsets_Batch-4_Live.fcs · COVID-RC_088_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_097_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_100_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_101_Post-1_ImmuneSubsets_Batch-5_Live.fcs · COVID-RC_105_Post-1_ImmuneSubsets_Batch-5_Live.fcs |
| · Healthy | Healthy_014__ImmuneSubsets_Batch-3_Live.fcs · Healthy_028__ImmuneSubsets_Batch-3_Live.fcs · Healthy_044__ImmuneSubsets_Batch-3_Live.fcs · Healthy_122__ImmuneSubsets_Batch-3_Live.fcs · Healthy_140__ImmuneSubsets_Batch-4_Live.fcs · Healthy_154__ImmuneSubsets_Batch-1_Live.fcs · Healthy_155__ImmuneSubsets_Batch-1_Live.fcs · Healthy_156__ImmuneSubsets_Batch-1_Live.fcs · Healthy_157__ImmuneSubsets_Batch-2_Live.fcs · Healthy_159__ImmuneSubsets_Batch-2_Live.fcs · Healthy_162__ImmuneSubsets_Batch-4_Live.fcs · Healthy_163__ImmuneSubsets_Batch-1_Live.fcs · Healthy_164__ImmuneSubsets_Batch-2_Live.fcs · Healthy_168__ImmuneSubsets_Batch-4_Live.fcs · Healthy_172__ImmuneSubsets_Batch-4_Live.fcs · Healthy_175__ImmuneSubsets_Batch-1_Live.fcs · Healthy_180__ImmuneSubsets_Batch-2_Live.fcs · Healthy_183__ImmuneSubsets_Batch-4_Live.fcs |
