Experiment Overview
| Repository ID: | FR-FCM-ZY2E | Experiment name: | Data aquisition of different input light intensities (quantiFlash) to PMT voltages from 400-1000V to determine laser background, signal-to-noise and dynamic range of FC channels | MIFlowCyt score: | 57.57% |
| Primary researcher: | Toralf Kaiser | PI/manager: | Toralf Kaiser | Uploaded by: | Toralf Kaiser |
| Experiment dates: | 2016-12-01 - 2017-01-31 | Dataset uploaded: | Feb 2017 | Last updated: | Sep 2017 |
| Keywords: | [quantiFlash] [instrument characterization] [dynamic range] [signal-to-noise] | Manuscripts: | [28960720] |
|
|
| Organizations: |
Deutsches Rheuma-Forschungszentrum Berlin, Flow Cytometry Core Facility (FCCF), Berlin, (Germany)
|
||||
| Purpose: | Data aquisition of different input light intensities (quantiFlash) to PMT voltages ranging from 400-1000V (and 50V increment steps) to determine laser background, signal-to-noise and dynamic range of FC channels, i.e. instrument characterization using a stable LED light source | ||||
| Conclusion: | a precision input-light source (quantiFlash) can be used to determine instruments background (by switching the lasers off), and SNR and DNR can be calculated from background to a reference light-input signal | ||||
| Comments: | None | ||||
| Funding: | Kristen Feher's contributions were made possible by European Union project 10158448EFRE. Claudia Giesecke's contributions were made possible by DFG funding through the Berlin-Brandenburg School for Regenerative Therapies GSC 203. | ||||
| Quality control: | we repeated these experiemnts and on different instruments and came to the same conslusions | ||||
