Experiment Overview
| Repository ID: | FR-FCM-ZY36 | Experiment name: | Chagas specific T cells TNF | MIFlowCyt score: | 84.48% |
| Primary researcher: | John Gonzalez | PI/manager: | John Gonzalez | Uploaded by: | John Gonzalez |
| Experiment dates: | 2014-01-12 - 2016-12-15 | Dataset uploaded: | Feb 2017 | Last updated: | Feb 2017 |
| Keywords: | [T cell] [cell surface markers] [Chagas disease] | Manuscripts: | |||
| Organizations: |
Universidad de los Andes, Medicine, Bogotá, DC (Colombia)
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| Purpose: | To determine the pool of antigen-specific T cells by the expression of membrane TNF-? and CD154. | ||||
| Conclusion: | Membrane TNF and CD154 (CD40L) are useful markers to assess T.cruzi specific T cell (TCD4+ and TCD8+) in chronic chagasic patients. | ||||
| Comments: | None | ||||
| Funding: | This work was partially supported by the Vicerectoría de Investigaciones, Universidad de los Andes, Colombia. | ||||
| Quality control: | CST Positive controls Antibodies titration | ||||
Experiment variables
| Conditions | |
|---|---|
| · SEB | TNF and CD154_CP1 SEB.fcs · TNF and CD154_CP2 SEB.fcs · TNF and CD154_CP3 SEB.fcs · TNF and CD154_CP4 SEB.fcs · TNF and CD154_CP5 SEB.fcs · TNF and CD154_CP6 SEB.fcs · TNF and CD154_CP7 SEB.fcs · TNF and CD154_CP8 SEB.fcs · TNF and CD154_HC1 SEB.fcs · TNF and CD154_HC2 SEB.fcs · TNF and CD154_HC3 SEB.fcs · TNF and CD154_HC4 SEB.fcs · TNF and CD154_HC5 SEB.fcs · TNF and CD154_HC6 SEB.fcs · TNF and CD154_HC7 SEB.fcs · TNF and CD154_NCC1 SEB.fcs · TNF and CD154_NCC2 SEB.fcs · TNF and CD154_NCC3 SEB.fcs |
| · alone | TNF and CD154_C35 alone.fcs · TNF and CD154_CP1 alone.fcs · TNF and CD154_CP2 alone.fcs · TNF and CD154_CP4 alone.fcs · TNF and CD154_CP5 alone.fcs · TNF and CD154_CP6 alone.fcs · TNF and CD154_CP7 alone.fcs · TNF and CD154_CP8 alone.fcs · TNF and CD154_HC1 alone.fcs · TNF and CD154_HC2 alone.fcs · TNF and CD154_HC3 alone.fcs · TNF and CD154_HC4 alone.fcs · TNF and CD154_HC5 alone.fcs · TNF and CD154_HC6 alone.fcs · TNF and CD154_HC7 alone.fcs · TNF and CD154_NCC1 alone.fcs · TNF and CD154_NCC2 alone.fcs · TNF and CD154_NCC3 alone.fcs |
| Sample Type | |
|---|---|
| · PBMC | TNF and CD154_C35 alone.fcs · TNF and CD154_CP1 SEB.fcs · TNF and CD154_CP1 alone.fcs · TNF and CD154_CP1 antigen.fcs · TNF and CD154_CP2 SEB.fcs · TNF and CD154_CP2 alone.fcs · TNF and CD154_CP2 antigen.fcs · TNF and CD154_CP3 SEB.fcs · TNF and CD154_CP3 antigen.fcs · TNF and CD154_CP4 SEB.fcs · TNF and CD154_CP4 alone.fcs · TNF and CD154_CP4 antigen.fcs · TNF and CD154_CP5 SEB.fcs · TNF and CD154_CP5 alone.fcs · TNF and CD154_CP5 antigen.fcs · TNF and CD154_CP6 SEB.fcs · TNF and CD154_CP6 alone.fcs · TNF and CD154_CP6 antigen.fcs · TNF and CD154_CP7 SEB.fcs · TNF and CD154_CP7 alone.fcs · TNF and CD154_CP7 antigen.fcs · TNF and CD154_CP8 SEB.fcs · TNF and CD154_CP8 alone.fcs · TNF and CD154_CP8 antigen.fcs · TNF and CD154_HC1 alone.fcs · TNF and CD154_HC1 antigen.fcs · TNF and CD154_HC1 SEB.fcs · TNF and CD154_HC2 SEB.fcs · TNF and CD154_HC2 alone.fcs · TNF and CD154_HC2 antigen.fcs · TNF and CD154_HC3 SEB.fcs · TNF and CD154_HC3 alone.fcs · TNF and CD154_HC3 antigen.fcs · TNF and CD154_HC4 SEB.fcs · TNF and CD154_HC4 alone.fcs · TNF and CD154_HC4 antigen.fcs · TNF and CD154_HC5 SEB.fcs · TNF and CD154_HC5 alone.fcs · TNF and CD154_HC5 antigen.fcs · TNF and CD154_HC6 SEB.fcs · TNF and CD154_HC6 alone.fcs · TNF and CD154_HC6 antigen.fcs · TNF and CD154_HC7 SEB.fcs · TNF and CD154_HC7 alone.fcs · TNF and CD154_HC7 antigen.fcs · TNF and CD154_NCC1 SEB.fcs · TNF and CD154_NCC1 alone.fcs · TNF and CD154_NCC1 antigen.fcs · TNF and CD154_NCC2 SEB.fcs · TNF and CD154_NCC2 alone.fcs · TNF and CD154_NCC2 antigen.fcs · TNF and CD154_NCC3 SEB.fcs · TNF and CD154_NCC3 alone.fcs · TNF and CD154_NCC3 antigen.fcs |
