Experiment Overview
| Repository ID: | FR-FCM-ZY3J | Experiment name: | 20161114-CyTOF analysis of Skeletal Muscle in Pax7 knock-out pups and controls | MIFlowCyt score: | 68.00% |
| Primary researcher: | Ermelinda Porpiglia | PI/manager: | Helen Blau | Uploaded by: | Ermelinda Porpiglia |
| Experiment dates: | 2016-11-14 - 2016-11-14 | Dataset uploaded: | Mar 2017 | Last updated: | Apr 2017 |
| Keywords: | [Adult Stem Cells] [Mutation] [CyTOF] [Skeletal Muscle] [Progenitor Cells ] | Manuscripts: | |||
| Organizations: |
Stanford University, Microbiology and Immunology, Stanford, CA (USA)
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| Purpose: | Investigate the distribution of stem and progenitor cell populations identified in skeletal muscle in Pax7 knock out neonates and controls. | ||||
| Conclusion: | The relative distribution of stem and progenitor cell populations is altered in Pax7 knock out neonates with a decrease in the fraction of stem cells and an increase in the fraction of progenitor cells. | ||||
| Comments: | CyTOF profile of Mouse Muscle Cell Isolates from Pax7 knockout neonates and controls | ||||
| Funding: | This study was supported by BD Biosciences stem cell grant (Ermelinda Porpiglia); US National Institutes of Health (NIH) grants NS089533, AG020961, and AG009521, California Institute for Regenerative Medicine grant RB5-07469 and the Baxter Foundation (Dr. Helen Blau) | ||||
| Quality control: | Visual inspection of biaxial plots | ||||
Experiment variables
| Conditions | |
|---|---|
| · Neonates | EP11142016-Pax7_Het_neonate_1.fcs · EP11142016-Pax7_Het_neonate_5.fcs · EP11142016-Pax7_Het_neonate_6.fcs · EP11142016-Pax7_Het_neonate_7.fcs · EP11142016-Pax7_Het_neonate_9.fcs · EP11142016-Pax7_knockout_neonate_2.fcs · EP11142016-WT_neonate_3.fcs · EP11142016-WT_neonate_4.fcs · EP11142016-WT_neonate_8.fcs |
| · 3 week-old | EP11142016-WT-3weeks_1.fcs · EP11142016-WT-3weeks_2.fcs · EP11142016-WT-3weeks_3.fcs · EP11142016-WT-3weeks_4.fcs |
| Sample Type | |
|---|---|
| · Wild type | EP11142016-WT-3weeks_1.fcs · EP11142016-WT-3weeks_2.fcs · EP11142016-WT-3weeks_3.fcs · EP11142016-WT-3weeks_4.fcs · EP11142016-WT_neonate_3.fcs · EP11142016-WT_neonate_4.fcs · EP11142016-WT_neonate_8.fcs |
| · Pax7 Het | EP11142016-Pax7_Het_neonate_1.fcs · EP11142016-Pax7_Het_neonate_5.fcs · EP11142016-Pax7_Het_neonate_6.fcs · EP11142016-Pax7_Het_neonate_7.fcs · EP11142016-Pax7_Het_neonate_9.fcs |
| · Pax7 knock out | EP11142016-Pax7_knockout_neonate_2.fcs |
