Experiment Overview
| Repository ID: | FR-FCM-ZY3K | Experiment name: | 20160824-CyTOF Analysis of Acute Injury Time Course in Skeletal Muscle | MIFlowCyt score: | 65.75% |
| Primary researcher: | Ermelinda Porpiglia | PI/manager: | Helen Blau | Uploaded by: | Ermelinda Porpiglia |
| Experiment dates: | 2016-08-24 - 2016-08-24 | Dataset uploaded: | Mar 2017 | Last updated: | Apr 2017 |
| Keywords: | [Adult Stem Cells] [CyTOF] [Skeletal Muscle] [Progenitor Cells ] [Injure] | Manuscripts: | |||
| Organizations: |
Stanford University, Microbiology and Immunology, Stanford, CA (USA)
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| Purpose: | To capture the progression of myogenesis in vivo we employed CyTOF to analyze the cellular and molecular dynamics during the course of an Acute Injury Time Course. | ||||
| Conclusion: | Unsupervised X-shift analysis of the myogenic compartment during the time-course of recovery from acute injury was instrumental in visualizing the spatial relationships between muscle stem cells and the newly identified progenitor cells, and in uncovering a dynamic myogenic trajectory that delineated cell-state transitions. | ||||
| Comments: | Comments: CyTOF profile of Mouse Muscle Cell Isolates in a Notexin Injury Time Course Experiment Sample Source include Tibialis Anterior (TA) and Gastrocnemius (GA) Muscles | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Visual Inspection of biaxial plots | ||||
Experiment variables
| Conditions | |
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| · Day 0 | EP20160824-InjuryTimeCourse-Day0_1.fcs · EP20160824-InjuryTimeCourse-Day0_2.fcs |
| · Day 3 | EP20160824-InjuryTimeCourse-Day3_1.fcs · EP20160824-InjuryTimeCourse-Day3_2.fcs |
| · Day 6 | EP20160824-InjuryTimeCourse-Day6_1.fcs · EP20160824-InjuryTimeCourse-Day6_2.fcs |
