Experiment Overview
| Repository ID: | FR-FCM-ZY3V | Experiment name: | Rapid Genome-wide Recruitment of RNA Polymerase II Drives Transcription, Splicing and Translation Events during T Cell Responses | MIFlowCyt score: | 59.74% |
| Primary researcher: | Johannes Lichti | PI/manager: | Elke Glasmacher | Uploaded by: | Johannes Lichti |
| Experiment dates: | 2014-08-04 - 2017-03-15 | Dataset uploaded: | Mar 2017 | Last updated: | Mar 2017 |
| Keywords: | [APOPTOSIS] [mouse T cells] [IFNg] [CD 4 T cells] [Th0] [Th1] [4sU-labeling] | Manuscripts: |
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| Organizations: |
HelmholtzZentrum, Munich, (Germany)
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| Purpose: | We wanted to verify optimal 4sU-labeling conditions and Ifng levels of activated Th0/Th1 cells. | ||||
| Conclusion: | No apoptosis of 4sU-labeled cells for assigned concentrations and time points of activated Th0 cells. Numbers of Ifnγ producing Th1 cells increase until 4h but decrease later on. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Each experiment was performed at least two times. BH3I-1 treatment was used to induce apoptosis (determined by Annexin V – PE), whereas heat shock (5min at 95°C) was used to induce cell death (determined by 7-AAD – PerCP). Unstained and single stained controls were included. | ||||
