Experiment Overview

Repository ID: FR-FCM-ZY3V Experiment name: Rapid Genome-wide Recruitment of RNA Polymerase II Drives Transcription, Splicing and Translation Events during T Cell Responses MIFlowCyt score: 59.74%
Primary researcher: Johannes Lichti PI/manager: Elke Glasmacher Uploaded by: Johannes Lichti
Experiment dates: 2014-08-04 - 2017-03-15 Dataset uploaded: Mar 2017 Last updated: Mar 2017
Keywords: [APOPTOSIS] [mouse T cells] [IFNg] [CD 4 T cells] [Th0] [Th1] [4sU-labeling] Manuscripts: Cellreportslogo
Organizations: HelmholtzZentrum, Munich, (Germany)
Purpose: We wanted to verify optimal 4sU-labeling conditions and Ifng levels of activated Th0/Th1 cells.
Conclusion: No apoptosis of 4sU-labeled cells for assigned concentrations and time points of activated Th0 cells. Numbers of Ifnγ producing Th1 cells increase until 4h but decrease later on.
Comments: None
Funding: Not disclosed
Quality control: Each experiment was performed at least two times. BH3I-1 treatment was used to induce apoptosis (determined by Annexin V – PE), whereas heat shock (5min at 95°C) was used to induce cell death (determined by 7-AAD – PerCP). Unstained and single stained controls were included.
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