Experiment Overview
| Repository ID: | FR-FCM-ZY3Z | Experiment name: | Multicenter Study-Gates Foundation | MIFlowCyt score: | 25.50% |
| Primary researcher: | Mike Leipold | PI/manager: | Holden Maecker | Uploaded by: | Mike Leipold |
| Experiment dates: | 2015-06-01 - | Dataset uploaded: | Feb 2017 | Last updated: | Nov 2017 |
| Keywords: | [flow cytometry] [mass cytometry] [CyTOF] [Standardization] | Manuscripts: | |||
| Organizations: | None | ||||
| Purpose: | The purpose of this experiment was to test the comparability of multiple sites to perform the same CyTOF assay. The central site shipped unstained cells, prestained cells, and concentrated premixed antibody cocktail to each site. The sites performed the experiment, acquired the data on their instruments, and sent the raw data files back to the central site for analysis. | ||||
| Conclusion: | Each site was able to perform the staining within designated parameters. The prestained cells and calibration beads allowed comparison of sensitivity. The premixed cocktail had significant problems with increased background in a few markers; premixed cocktail should NOT be used in future studies. | ||||
| Comments: | The premixed cocktail had significant problems with increased background in a few markers; in the future, premixed cocktail should NOT be used. There were also issues with parameter naming and with file-naming; future studies will hopefully avoid some of these issues through the use of panel template files. | ||||
| Funding: | Bill and Melinda Gates Foundation, GHVAP | ||||
| Quality control: | Inclusion of Fluidigm EQ normalization beads within samples. Use of prestained cells generated by the central site. Splitting the same stained sample in two, and running each half on sequential days. | ||||
