Experiment Overview
| Repository ID: | FR-FCM-ZY4P | Experiment name: | CyTOF immuno-profiling of regenerating lacrimal glands | MIFlowCyt score: | 100.00% |
| Primary researcher: | Driss Zoukhri | PI/manager: | Driss Zoukhri | Uploaded by: | Dillon Hawley |
| Experiment dates: | 2014-06-01 - 2014-09-01 | Dataset uploaded: | Apr 2017 | Last updated: | May 2017 |
| Keywords: | [Innate Immune Response] [inflammation] [lacrimal gland] | Manuscripts: |
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| Organizations: |
Tufts Dental School, Department of Comprehensive Care, Boston, MA (United States)
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| Purpose: | CyTOF immuno-profiling was used to identify cells involved the immune response using an experimental model of lacrimal gland inflammation (IL-1alpha). | ||||
| Conclusion: | Natural Killer cell populations showed minor increases during the regenerative phases, while, B and T cell populations showed no major changes compared to uninjured LGs. Neutrophils and monocytes showed large increased in 1 and 2 days post injury identifying the innate immune system as the key mediator of the inflammatory response in acute LG injury. | ||||
| Comments: | None | ||||
| Funding: | See linked paper. | ||||
| Quality control: | Samples were composed of three biological replicates and normalized to internal beads control. | ||||
Experiment variables
| Doses | |
|---|---|
| · Un-injured | Day 0_Control.fcs |
| · 1ug IL-1alpha | Day 1.fcs · Day 2.fcs · Day 3.fcs · Day 4.fcs · Day 5.fcs · Day 6.fcs · Day 7.fcs |
| Timepoints | |
|---|---|
| · 1 day | Day 1.fcs |
| · 2 day | Day 2.fcs |
| · 3 day | Day 3.fcs |
| · 4 day | Day 4.fcs |
| · 5 day | Day 5.fcs |
| · 6 day | Day 6.fcs |
| · 7 day | Day 7.fcs |
