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Experiment Overview

Repository ID: FR-FCM-ZY69 Experiment name: MC38 Checkpoint Blockade MIFlowCyt score: 46.25%
Primary researcher: Spencer Wei PI/manager: Spencer Wei Uploaded by: Spencer Wei
Experiment dates: 2017-05-02 - Dataset uploaded: May 2017 Last updated: Aug 2017
Keywords: [mass cytometry] [CyTOF] [mouse] [tumor] [PD-1] [TIL] [CTLA-4] [checkpoint blockade] Manuscripts:
Organizations: The University of Texas MD Anderson Cancer Center, Immunology, Houston, Texas (USA)
Purpose: To comprehensively profile tumor infiltrating T cells and identify checkpoint blockade responsive T cell subsets by mass cytometry.
Conclusion: None
Comments: Individual tumor infiltrates were analyzed by mass cytometry. TIL samples were barcoded and acquired simultaneously.
Funding: Not disclosed
Quality control: None


Experiment variables

Sample Type
· Bead normalized and debarcoded un-gated data c01_2016-0829-MC38AbTx_norm.fcs · c02_2016-0829-MC38AbTx_norm.fcs · c03_2016-0829-MC38AbTx_norm.fcs · c04_2016-0829-MC38AbTx_norm.fcs · c05_2016-0829-MC38AbTx_norm.fcs · c06_2016-0829-MC38AbTx_norm.fcs · c07_2016-0829-MC38AbTx_norm.fcs · c08_2016-0829-MC38AbTx_norm.fcs · c09_2016-0829-MC38AbTx_norm.fcs · c10_2016-0829-MC38AbTx_norm.fcs · c11_2016-0829-MC38AbTx_norm.fcs · c12_2016-0829-MC38AbTx_norm.fcs · c13_2016-0829-MC38AbTx_norm.fcs · c14_2016-0829-MC38AbTx_norm.fcs · c15_2016-0829-MC38AbTx_norm.fcs · c16_2016-0829-MC38AbTx_norm.fcs · c17_2016-0829-MC38AbTx_norm.fcs · c18_2016-0829-MC38AbTx_norm.fcs · c19_2016-0829-MC38AbTx_norm.fcs · c20_2016-0829-MC38AbTx_norm.fcs
· T cell gated data with PhenoGraph and t-SNE parameters 20160829 MC38 AbTx2 CD3 C312.fcs · 20160829 MC38 AbTx2 CD3 C313.fcs · 20160829 MC38 AbTx2 CD3 C314.fcs · 20160829 MC38 AbTx2 CD3 C315.fcs · 20160829 MC38 AbTx2 CD3 C316.fcs · 20160829 MC38 AbTx2 CD3 C317.fcs · 20160829 MC38 AbTx2 CD3 C318.fcs · 20160829 MC38 AbTx2 CD3 C319.fcs · 20160829 MC38 AbTx2 CD3 C320.fcs · 20160829 MC38 AbTx2 CD3 C321.fcs · 20160829 MC38 AbTx2 CD3 C322.fcs · 20160829 MC38 AbTx2 CD3 C323.fcs · 20160829 MC38 AbTx2 CD3 C324.fcs · 20160829 MC38 AbTx2 CD3 C325.fcs · 20160829 MC38 AbTx2 CD3 C326.fcs · 20160829 MC38 AbTx2 CD3 C327.fcs · 20160829 MC38 AbTx2 CD3 C328.fcs · 20160829 MC38 AbTx2 CD3 C329.fcs · 20160829 MC38 AbTx2 CD3 C330.fcs · 20160829 MC38 AbTx2 CD3 C331.fcs

Conditions
· Control 20160829 MC38 AbTx2 CD3 C312.fcs · 20160829 MC38 AbTx2 CD3 C313.fcs · 20160829 MC38 AbTx2 CD3 C314.fcs · 20160829 MC38 AbTx2 CD3 C315.fcs · c01_2016-0829-MC38AbTx_norm.fcs · c02_2016-0829-MC38AbTx_norm.fcs · c03_2016-0829-MC38AbTx_norm.fcs · c04_2016-0829-MC38AbTx_norm.fcs
· anti-CTLA-4 20160829 MC38 AbTx2 CD3 C316.fcs · 20160829 MC38 AbTx2 CD3 C317.fcs · 20160829 MC38 AbTx2 CD3 C318.fcs · 20160829 MC38 AbTx2 CD3 C319.fcs · 20160829 MC38 AbTx2 CD3 C320.fcs · 20160829 MC38 AbTx2 CD3 C321.fcs · 20160829 MC38 AbTx2 CD3 C322.fcs · 20160829 MC38 AbTx2 CD3 C323.fcs · c05_2016-0829-MC38AbTx_norm.fcs · c06_2016-0829-MC38AbTx_norm.fcs · c07_2016-0829-MC38AbTx_norm.fcs · c08_2016-0829-MC38AbTx_norm.fcs · c09_2016-0829-MC38AbTx_norm.fcs · c10_2016-0829-MC38AbTx_norm.fcs · c11_2016-0829-MC38AbTx_norm.fcs · c12_2016-0829-MC38AbTx_norm.fcs
· anti-PD-1 20160829 MC38 AbTx2 CD3 C324.fcs · 20160829 MC38 AbTx2 CD3 C325.fcs · 20160829 MC38 AbTx2 CD3 C326.fcs · 20160829 MC38 AbTx2 CD3 C327.fcs · 20160829 MC38 AbTx2 CD3 C328.fcs · 20160829 MC38 AbTx2 CD3 C329.fcs · 20160829 MC38 AbTx2 CD3 C330.fcs · 20160829 MC38 AbTx2 CD3 C331.fcs · c13_2016-0829-MC38AbTx_norm.fcs · c14_2016-0829-MC38AbTx_norm.fcs · c15_2016-0829-MC38AbTx_norm.fcs · c16_2016-0829-MC38AbTx_norm.fcs · c17_2016-0829-MC38AbTx_norm.fcs · c18_2016-0829-MC38AbTx_norm.fcs · c19_2016-0829-MC38AbTx_norm.fcs · c20_2016-0829-MC38AbTx_norm.fcs

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