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Experiment Overview

Repository ID: FR-FCM-ZY6D Experiment name: OMIP-040: Henry et al - CytoPartA - 2017 - huEpi MIFlowCyt score: 87.33%
Primary researcher: Gervaise Henry PI/manager: Gervaise Henry Uploaded by: Gervaise Henry
Experiment dates: 2017-01-01 - 2017-05-01 Dataset uploaded: May 2017 Last updated: Aug 2017
Keywords: [Human prostate] [Benign prostatic hyperplasia] [Epithelia] [Tissue phenotyping] Manuscripts: Cytalogo
Organizations: University of Texas Southwestern Medical Center, Department of Urology, Dallas, TX (USA)
University of Texas Southwestern Medical Center, 1.4.1.2. The Moody Foundation Flow Cytometry Facility, Children’s Medical Center Research Institute, Dallas, TX (USA)
Purpose: Quantification and characterization of the major cell populations in primary human prostate tissues via cell surface markers.
Conclusion: Epithelia, stoma and leukocytes can be isolated using CD45 and CD326. Epithelia are CD326Pos/CD45Neg, leukocytes are CD45Pos/CD26Neg, while stroma are double negative for these markers. Stroma can be separated into endothelia and fibromuscular stroma with CD31. Endothelia are CD31Pos while fibromuscular stroma are CD31Neg. Epithelia can also be divided into basal and luminal epithelia using the basal marker CD271 and the luminal marker CD26.
Comments: None
Funding: Not disclosed
Quality control: To standardize voltage settings across samples acquired on different days, CS&T was run on the instrument daily. Also, PMT voltages were adjusted such that the median fluorescent intensity of peak number 4 of Rainbow Calibration Particles (BioLegend, Cat# 422901) was identical for each channel used, regardless of date of acquisition.


Experiment variables

Individuals
· Human Prostate Cells Strategy_D3Tz_010.fcs
· Calibration Beads Voltage Setup_Rainbow6-B203387_001.fcs

Sample Type
· Cells Strategy_D3Tz_010.fcs
· Beads Voltage Setup_Rainbow6-B203387_001.fcs

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