Experiment Overview
| Repository ID: | FR-FCM-ZY6X | Experiment name: | Figure2. Plots showing specificity of fluorescent proteins co-expressed within 293T cells overlaid with single fluorophore expression data. | MIFlowCyt score: | 65.50% |
| Primary researcher: | Andre Olsson | PI/manager: | Andre Olsson | Uploaded by: | Andre Olsson |
| Experiment dates: | 2017-03-17 - 2017-03-17 | Dataset uploaded: | May 2017 | Last updated: | Mar 2018 |
| Keywords: | [Fluorescent proteins] [Compensation] [mice] [Genetic Reporters] | Manuscripts: | [29533508] |
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| Organizations: |
Cincinnati Children's, CCHMC, Immunobiology, Cinccinati, Ohio (United States)
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| Purpose: | To asses functional and bleed over properties between fluorescent proteins by coexpressing them | ||||
| Conclusion: | Up to four different fluorescent proteins could be combined without compensatory issues. Optimally use of BFP2 with IFP/TagRFP657 and Apple/mCherry/Tomato and Venus/YFP should result in good separation characteristics. Avoid GFP. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Experiments were repeated multiple times. | ||||
