Experiment Overview

Repository ID: FR-FCM-ZY7C Experiment name: Flow Cytometric Analysis of variation and dynamics of salivary microbiota MIFlowCyt score: 31.54%
Primary researcher: Florian Schattenberg PI/manager: Florian Schattenberg Uploaded by: Florian Schattenberg
Experiment dates: 2015-09-14 - 2017-01-25 Dataset uploaded: Jun 2017 Last updated: Jul 2017
Keywords: [microbial flow cytometry] [saliva] [mouth microbiome] [cell counting] [microbial diversity]] Manuscripts: [28842259]
Organizations: Helmholtz Center for Environmental Research, Department of Environmental Microbiology, Working Group Flow Cytometry, Leipzig, (Germany)
University of Leipzig, Department of Cariology, Endodontology and Periodontology, Leipzig, (Germany)
Purpose: Microbial flow cytometry is an established fast and economic technique for complex ecosystem studies (Zimmermann et al., European Journal of Immunology, 2016) and enables visualization of rapidly changing community structures by measuring characteristics of single microbial cells (Koch et al., Frontiers in Microbiology,2014; Koch et al., Nature Protocols, 2013). We established a cytometric workflow to routinely analyze salivary microbiomes on the example of ten oral healthy subjects
Conclusion: Flow cytometry enables an illustration of complex bacterial compartments in the salivary microbiome and a monitoring of its shifts. The examination of ten test person’s saliva reveals individual profiles of their microbial communities. Intraindividual stabilities, interindividual diversities and dynamics correlated to extrinsic stress impacts are discernible.
Comments: None
Funding: This work was supported by the Genome Analytics Group at Helmholtz Centre for Infection Research in Braunschweig (GMAK, HZI, Braunschweig, Germany), the Helmholtz Centre for Environmental Research (UFZ), the Central Innovation Programme for SMEs (ZIM) of the federal ministry of economic affairs and energy (BMWi) (INAR-ABOS, 16KN043222), the Federal Ministry of Education and Research (BMBF) (WiPro-Wissensbasierte Prozessintelligenz, 031A616K) and the European Regional Development Funds (EFRE – Europe funds Saxony, 100192205).
Quality control: Fluorescent beads FluoSpheres 1 µm (F8815 (350/440), lot no.: 69A1-1, Molecular Probes Eugene, Oregon, USA), FluoSpheres 2 µm (F-8827 (505/515), lot no.: 1717426, Molecular Probes Eugene, Oregon, USA), and blue fluorescent beads Fluoresbrite BB Carboxylate microspheres 0.5 μm (360/407, lot no.: 552744, PolyScience, Niles, Illinois, USA), 1 μm (360/407, lot no.: 499344, PolyScience, Niles, Illinois, USA) were used to align the instrumental settings
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