Experiment Overview
| Repository ID: | FR-FCM-ZY7E | Experiment name: | Fitness of 14 TDH3 promoter variants in YPD | MIFlowCyt score: | 49.25% |
| Primary researcher: | Fabien Duveau | PI/manager: | Patricia Wittkopp | Uploaded by: | Fabien Duveau |
| Experiment dates: | 2014-04-19 - 2016-04-10 | Dataset uploaded: | Jun 2017 | Last updated: | Aug 2017 |
| Keywords: | [Gene expression] [fitness optimum] [robustness. ] | Manuscripts: | |||
| Organizations: |
University of Michigan, Ann Arbor, MI (USA)
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| Purpose: | Determining the fitness consequences of changes in the expression level of the yeast gene TDH3. | ||||
| Conclusion: | The relationship between TDH3 expression level and fitness in YPD is non-linear and presents a plateau of maximal fitness located around the wild type level of expression. | ||||
| Comments: | Fitness was quantified by competition between each tested strain expressing YFP against a common competitor expressing GFP. The relative frequency of YFP and GFP cells present in each population was recorded at four time points. | ||||
| Funding: | European Molecular Biology Organization postdoctoral fellowship (EMBO ALTF 1114-2012), National Science Foundation (MCB-1021398) and National Institutes of Health (R01GM108826 and 1R35GM118073) grants. | ||||
| Quality control: | The FCS data were collected from seven plates of 96 samples scored at four different time points and in 3 separate experiments including one or three plates each. Each plate contains 24 replicates of the control YFP strain with a wild type copy of TDH3 competed against the GFP competitor. These controls can be used to correct for variation existing between plates. | ||||
