Experiment Overview
| Repository ID: | FR-FCM-ZY7U | Experiment name: | Canine PMSC characterization | MIFlowCyt score: | 49.25% |
| Primary researcher: | Lee Lankford | PI/manager: | Aijun Wang | Uploaded by: | Lee Lankford |
| Experiment dates: | 2015-10-01 - 2016-02-25 | Dataset uploaded: | Jul 2017 | Last updated: | Jul 2017 |
| Keywords: | [characterization] [Mesenchymal stem cells] [Placenta] | Manuscripts: | [28715613] |
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| Organizations: |
University of California, Davis Medical Center, Surgery, Sacramento, CA (United States)
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| Purpose: | To perform immunophenotypic characterization of canine placenta-derived mesenchymal stromal cells (cPMSCs) | ||||
| Conclusion: | Our conclusions drawn from our flow cytometry immunophenotype analysis of cPMSCs are that they are immunoreactive for commonly positive MSC markers CD90, CD44, and CD29 and are non-reactive for common hematopoietic and endothelial lineage markers CD45, CD34, and CD31 | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | All antibodies had concentration matched isotype control samples, which were used to set negative gates. In the case of CD45, an antibody which was provided by Peter Moore's lab at UC Davis, anti-feline CD4 antibody at matching concentration was used as a negative control. | ||||
