Experiment Overview
| Repository ID: | FR-FCM-ZY9N | Experiment name: | CyTOF analysis of paired blood, tumor and non-involved lung from NSCLC patients | MIFlowCyt score: | 60.86% |
| Primary researcher: | Adeeb Rahman | PI/manager: | Adeeb Rahman | Uploaded by: | Adeeb Rahman |
| Experiment dates: | 2015-03-04 - 2016-03-11 | Dataset uploaded: | Aug 2017 | Last updated: | Aug 2017 |
| Keywords: | [mass cytometry] [CyTOF] [NSCLC] [adenocarcinoma] | Manuscripts: | [28475900] | ||
| Organizations: |
Icahn School of Medicine at Mt. Sinai, New York, NY (10029)
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| Purpose: | To perform a detailed characterization of the immune landscape of paired blood, tumor and non-involved lung tissue from NSCLC patients | ||||
| Conclusion: | None | ||||
| Comments: | CD45-barcoded populations from each tissue were deconvolved by Boolean gating. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Single cell suspensions from paired blood, tumor and non-involved lung were barcoded with CD45 antibodies conjugated to distinct isotopes and pooled prior to staining and acquisition to minimize experimental variation between paired samples. | ||||
Experiment variables
| Individuals | |
|---|---|
| · 236 | 236_myeloid_blood.fcs · 236_myeloid_lung.fcs · 236_myeloid_tumor.fcs |
| · 237 | 237_myeloid_blood.fcs · 237_myeloid_lung.fcs · 237_myeloid_tumor.fcs |
| · 245 | 245_myeloid_blood.fcs · 245_myeloid_lung.fcs · 245_myeloid_tumor.fcs |
| · 246 | 246_myeloid_blood.fcs · 246_myeloid_lung.fcs · 246_myeloid_tumor.fcs |
| · 249 | 249_myeloid_blood.fcs · 249_myeloid_lung.fcs · 249_myeloid_tumor.fcs |
| · 250 | 250_myeloid_blood.fcs · 250_myeloid_lung.fcs · 250_myeloid_tumor.fcs |
| · 251 | 251_myeloid_blood.fcs · 251_myeloid_lung.fcs · 251_myeloid_tumor.fcs |
| · 253 | 253_myeloid_blood.fcs · 253_myeloid_lung.fcs · 253_myeloid_tumor.fcs |
| · 255 | 255_myeloid_blood.fcs · 255_myeloid_lung.fcs · 255_myeloid_tumor.fcs |
| · 256 | 256_myeloid_blood.fcs · 256_myeloid_lung.fcs · 256_myeloid_tumor.fcs |
| · 258 | 258_myeloid_blood.fcs · 258_myeloid_lung.fcs · 258_myeloid_tumor.fcs |
| · 259 | 259_myeloid_blood.fcs · 259_myeloid_lung.fcs · 259_myeloid_tumor.fcs |
| · 260 | 260_myeloid_blood.fcs · 260_myeloid_lung.fcs · 260_myeloid_tumor.fcs |
| · 261 | 261_myeloid_blood.fcs · 261_myeloid_lung.fcs · 261_myeloid_tumor.fcs |
| · 262 | 262_myeloid_blood.fcs · 262_myeloid_lung.fcs · 262_myeloid_tumor.fcs |
| · 263 | 263_myeloid_blood.fcs · 263_myeloid_lung.fcs · 263_myeloid_tumor.fcs |
| · 266 | 266_myeloid_blood.fcs · 266_myeloid_lung.fcs · 266_myeloid_tumor.fcs |
| · 267 | 267_myeloid_blood.fcs · 267_myeloid_lung.fcs · 267_myeloid_tumor.fcs |
| · 291 | 291_myeloid_blood.fcs · 291_myeloid_lung.fcs · 291_myeloid_tumor.fcs |
| · 296 | 296_Myeloid_blood.fcs · 296_Myeloid_lung.fcs · 296_Myeloid_tumor.fcs |
| Sample Type | |
|---|---|
| · blood | 236_myeloid_blood.fcs · 237_myeloid_blood.fcs · 245_myeloid_blood.fcs · 246_myeloid_blood.fcs · 249_myeloid_blood.fcs · 250_myeloid_blood.fcs · 251_myeloid_blood.fcs · 253_myeloid_blood.fcs · 255_myeloid_blood.fcs · 256_myeloid_blood.fcs · 258_myeloid_blood.fcs · 259_myeloid_blood.fcs · 260_myeloid_blood.fcs · 261_myeloid_blood.fcs · 262_myeloid_blood.fcs · 263_myeloid_blood.fcs · 266_myeloid_blood.fcs · 267_myeloid_blood.fcs · 291_myeloid_blood.fcs · 296_Myeloid_blood.fcs |
| · lung | 236_myeloid_lung.fcs · 237_myeloid_lung.fcs · 245_myeloid_lung.fcs · 246_myeloid_lung.fcs · 249_myeloid_lung.fcs · 250_myeloid_lung.fcs · 251_myeloid_lung.fcs · 253_myeloid_lung.fcs · 255_myeloid_lung.fcs · 256_myeloid_lung.fcs · 258_myeloid_lung.fcs · 259_myeloid_lung.fcs · 260_myeloid_lung.fcs · 261_myeloid_lung.fcs · 262_myeloid_lung.fcs · 263_myeloid_lung.fcs · 266_myeloid_lung.fcs · 267_myeloid_lung.fcs · 291_myeloid_lung.fcs · 296_Myeloid_lung.fcs |
| · tumor | 236_myeloid_tumor.fcs · 237_myeloid_tumor.fcs · 245_myeloid_tumor.fcs · 246_myeloid_tumor.fcs · 249_myeloid_tumor.fcs · 250_myeloid_tumor.fcs · 251_myeloid_tumor.fcs · 253_myeloid_tumor.fcs · 255_myeloid_tumor.fcs · 256_myeloid_tumor.fcs · 258_myeloid_tumor.fcs · 259_myeloid_tumor.fcs · 260_myeloid_tumor.fcs · 261_myeloid_tumor.fcs · 262_myeloid_tumor.fcs · 263_myeloid_tumor.fcs · 266_myeloid_tumor.fcs · 267_myeloid_tumor.fcs · 291_myeloid_tumor.fcs · 296_Myeloid_tumor.fcs |
