Experiment Overview
| Repository ID: | FR-FCM-ZY9X | Experiment name: | Spontaneous cytokine production from paired blood, tumor and non-involved lung from NSCLC patients | MIFlowCyt score: | 62.00% |
| Primary researcher: | Adeeb Rahman | PI/manager: | Adeeb Rahman | Uploaded by: | Adeeb Rahman |
| Experiment dates: | 2016-02-09 - 2016-06-10 | Dataset uploaded: | Aug 2017 | Last updated: | Aug 2017 |
| Keywords: | [Intracellular Cytokine Staining] [mass cytometry] [CyTOF] [NSCLC] | Manuscripts: | [28475900] | ||
| Organizations: |
Icahn School of Medicine at Mount Sinai , New York, NY (United States of America)
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| Purpose: | To characterize spontaneous cytokine production from immune populations from paired blood, tumor and non-involved lung tissue from NSCLC patients | ||||
| Conclusion: | None | ||||
| Comments: | Single cell suspensions from blood, tumor and lung were incubated for 4-6hrs in Brefeldin A. Samples were stained for viability and surface markers, and matched sets of samples from a single patient were then fixed and barcoded using the Fluidigm Pd barcoding kit and pooled prior to intracellular staining for cytokines. Data were normalized and debarcoded using Fluidigm software. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Individuals | |
|---|---|
| · 301 | lung_cytokine_301_Blood_BFA_live.fcs · lung_cytokine_301_Lung_BFA_live.fcs · lung_cytokine_301_Tumor_BFA_live.fcs |
| · 305 | lung_cytokine_305_Blood_BFA_live.fcs · lung_cytokine_305_Lung_BFA_live.fcs · lung_cytokine_305_Tumor_BFA_live.fcs |
| · 312 | lung_cytokine_312_Blood_BFA_live.fcs · lung_cytokine_312_Lung_BFA_live.fcs · lung_cytokine_312_Tumor_BFA_live.fcs |
| · 315 | lung_cytokine_315_Blood_BFA_live.fcs · lung_cytokine_315_Lung_BFA_live.fcs · lung_cytokine_315_Tumor_BFA_live.fcs |
| · 324 | lung_cytokine_324_Blood_BFA_live.fcs · lung_cytokine_324_Lung_BFA_live.fcs · lung_cytokine_324_Tumor_BFA_live.fcs |
| · 326 | lung_cytokine_326_Blood_BFA_live.fcs · lung_cytokine_326_Lung_BFA_live.fcs · lung_cytokine_326_Tumor_BFA_live.fcs |
| · 327 | lung_cytokine_327_Blood_BFA_live.fcs · lung_cytokine_327_Lung_BFA_live.fcs · lung_cytokine_327_Tumor_BFA_live.fcs |
| · 328 | lung_cytokine_328_Blood_BFA_live.fcs · lung_cytokine_328_Lung_BFA_live.fcs · lung_cytokine_328_Tumor_BFA_live.fcs |
| · 329 | lung_cytokine_329_Blood_BFA_live.fcs · lung_cytokine_329_Lung_BFA_live.fcs · lung_cytokine_329_Tumor_BFA_live.fcs |
| Sample Type | |
|---|---|
| · blood | lung_cytokine_301_Blood_BFA_live.fcs · lung_cytokine_305_Blood_BFA_live.fcs · lung_cytokine_312_Blood_BFA_live.fcs · lung_cytokine_315_Blood_BFA_live.fcs · lung_cytokine_324_Blood_BFA_live.fcs · lung_cytokine_326_Blood_BFA_live.fcs · lung_cytokine_327_Blood_BFA_live.fcs · lung_cytokine_328_Blood_BFA_live.fcs · lung_cytokine_329_Blood_BFA_live.fcs |
| · lung | lung_cytokine_301_Lung_BFA_live.fcs · lung_cytokine_305_Lung_BFA_live.fcs · lung_cytokine_312_Lung_BFA_live.fcs · lung_cytokine_315_Lung_BFA_live.fcs · lung_cytokine_324_Lung_BFA_live.fcs · lung_cytokine_326_Lung_BFA_live.fcs · lung_cytokine_327_Lung_BFA_live.fcs · lung_cytokine_328_Lung_BFA_live.fcs · lung_cytokine_329_Lung_BFA_live.fcs |
| · tumor | lung_cytokine_301_Tumor_BFA_live.fcs · lung_cytokine_305_Tumor_BFA_live.fcs · lung_cytokine_312_Tumor_BFA_live.fcs · lung_cytokine_315_Tumor_BFA_live.fcs · lung_cytokine_324_Tumor_BFA_live.fcs · lung_cytokine_326_Tumor_BFA_live.fcs · lung_cytokine_327_Tumor_BFA_live.fcs · lung_cytokine_328_Tumor_BFA_live.fcs · lung_cytokine_329_Tumor_BFA_live.fcs |
