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Experiment Overview

Repository ID: FR-FCM-ZYAJ Experiment name: HIMC PBMC controls-CyTOF surface phenotyping MIFlowCyt score: 35.00%
Primary researcher: Mike Leipold PI/manager: Mike Leipold Uploaded by: Mike Leipold
Experiment dates: 2016-08-11 - 2016-08-15 Dataset uploaded: Aug 2017 Last updated: Oct 2017
Keywords: [healthy controls] [human PBMCs] [CyTOF] Manuscripts:
Organizations: None
Purpose: PBMCs were isolated by Ficoll from LRS chambers obtained from the Stanford Blood center, then cryopreserved in FBS+10% DMSO in the vapor phase of liquid nitrogen until use. PBMC aliquots were thawed, washed, stained with the HIMC surface phenotyping 33 marker CyTOF panel, EQ normalization beads were added, and then were run on a Helios mass cytometer using the default settings. The resulting Raw data files were normalized separately in two ways: MATLAB (Finck et al) and Fluidigm-ver2.
Conclusion: None
Comments: None
Funding: Not disclosed
Quality control: None
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